Genomic Organization of Repetitive DNAs and Differentiation of an XX/XY Sex Chromosome System in the Amazonian Puffer Fish, Colomesus asellus (Tetraodontiformes)
The genus Colomesus is the sole representative of the family Tetraodontidae in the Amazon region. Here, Colomesus asellus was analyzed using conventional and molecular cytogenetic protocols. Its diploid chromosome number is 2n = 46 with 12 meta-, 10 submeta-, 16 subtelo-, and 8 acrocentric chromosom...
| Autor principal: | Viana, Patrik Ferreira |
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| Outros Autores: | Ezaz, Tariq Tariq, Marajó, Leandro, Ferreira, Milena, Zuanon, Jansen, Cioffi, Marcelo de Bello, Bertollo, Luiz Antônio Carlos, Gross, Maria Claudia, Feldberg, Eliana |
| Grau: | Artigo |
| Idioma: | English |
| Publicado em: |
Cytogenetic and Genome Research
2020
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| Acesso em linha: |
https://repositorio.inpa.gov.br/handle/1/16988 |
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oai:repositorio:1-16988 |
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oai:repositorio:1-16988 Genomic Organization of Repetitive DNAs and Differentiation of an XX/XY Sex Chromosome System in the Amazonian Puffer Fish, Colomesus asellus (Tetraodontiformes) Viana, Patrik Ferreira Ezaz, Tariq Tariq Marajó, Leandro Ferreira, Milena Zuanon, Jansen Cioffi, Marcelo de Bello Bertollo, Luiz Antônio Carlos Gross, Maria Claudia Feldberg, Eliana Dna 18s Repetitive Dna Tropomyosin Cell Nucleus Antigen Nucleolar Organizer Region Associated Proteins Ribosome Dna Rna 18s Tropomyosin Acrocentric Chromosome C Banding Chromosome Nor Chromosome Number Cytogenetics Diploidy Female In Situ Hybridization, Fluorescence Heterochromatin Karyotype Male Nonhuman Priority Journal Puffer Fish Sex Chromosome Telomere X Chromosome Y Chromosome Animals Brasil Chromosome Banding Pattern Gene Amplification Genetics Nucleotide Repeat Sex Chromosome Sex Determination Processes Tetraodontiformes Ultrastructure Animal Antigens, Nuclear Brasil Chromosome Banding Ribosomal Dna Female Gene Amplification In Situ Hybridization, Fluorescence Male Repetitive Sequences, Nucleic Acid Rna, Ribosomal, 18s Sex Chromosomes Sex Determination Processes Telomere Tetraodontiformes Tropomyosin The genus Colomesus is the sole representative of the family Tetraodontidae in the Amazon region. Here, Colomesus asellus was analyzed using conventional and molecular cytogenetic protocols. Its diploid chromosome number is 2n = 46 with 12 meta-, 10 submeta-, 16 subtelo-, and 8 acrocentric chromosomes and a fundamental number of FN = 84. An XX/XY sex chromosome system was identified. Mapping of 18S rDNA correlated with the nucleolus organizer regions (Ag-NORs) in the short arms of the 2 X chromosomes in females and in the Y chromosome in males. C-banding revealed heterochromatin in the centromeric regions of all chromosomes, except for pair 3. Prominent sex chromosome-specific heterochromatin amplification was observed, covering the short arms of the Y chromosome almost entirely. FISH with telomeric and tropomyosin (tpm1) sequences, respectively, revealed terminal signals in all chromosomes. The analysis of extended DNA fibers confirmed the colocalization and the interspersed pattern of the telomeric and tpm1 sequences. Thus, this study highlights the remarkable evolutionary dynamism presented by the Amazonian puffer fish regarding the differentiation of a heteromorphic XY sex chromosome system and a particular sex-specific amplification of rDNA sites. This is the first record of such an association in the Tetraodontidae family. © 2018 S. Karger AG, Basel. 2020-06-15T21:37:52Z 2020-06-15T21:37:52Z 2018 Artigo https://repositorio.inpa.gov.br/handle/1/16988 10.1159/000484423 en Volume 153, Número 2, Pags. 96-104 Restrito Cytogenetic and Genome Research |
| institution |
Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional |
| collection |
INPA-RI |
| language |
English |
| topic |
Dna 18s Repetitive Dna Tropomyosin Cell Nucleus Antigen Nucleolar Organizer Region Associated Proteins Ribosome Dna Rna 18s Tropomyosin Acrocentric Chromosome C Banding Chromosome Nor Chromosome Number Cytogenetics Diploidy Female In Situ Hybridization, Fluorescence Heterochromatin Karyotype Male Nonhuman Priority Journal Puffer Fish Sex Chromosome Telomere X Chromosome Y Chromosome Animals Brasil Chromosome Banding Pattern Gene Amplification Genetics Nucleotide Repeat Sex Chromosome Sex Determination Processes Tetraodontiformes Ultrastructure Animal Antigens, Nuclear Brasil Chromosome Banding Ribosomal Dna Female Gene Amplification In Situ Hybridization, Fluorescence Male Repetitive Sequences, Nucleic Acid Rna, Ribosomal, 18s Sex Chromosomes Sex Determination Processes Telomere Tetraodontiformes Tropomyosin |
| spellingShingle |
Dna 18s Repetitive Dna Tropomyosin Cell Nucleus Antigen Nucleolar Organizer Region Associated Proteins Ribosome Dna Rna 18s Tropomyosin Acrocentric Chromosome C Banding Chromosome Nor Chromosome Number Cytogenetics Diploidy Female In Situ Hybridization, Fluorescence Heterochromatin Karyotype Male Nonhuman Priority Journal Puffer Fish Sex Chromosome Telomere X Chromosome Y Chromosome Animals Brasil Chromosome Banding Pattern Gene Amplification Genetics Nucleotide Repeat Sex Chromosome Sex Determination Processes Tetraodontiformes Ultrastructure Animal Antigens, Nuclear Brasil Chromosome Banding Ribosomal Dna Female Gene Amplification In Situ Hybridization, Fluorescence Male Repetitive Sequences, Nucleic Acid Rna, Ribosomal, 18s Sex Chromosomes Sex Determination Processes Telomere Tetraodontiformes Tropomyosin Viana, Patrik Ferreira Genomic Organization of Repetitive DNAs and Differentiation of an XX/XY Sex Chromosome System in the Amazonian Puffer Fish, Colomesus asellus (Tetraodontiformes) |
| topic_facet |
Dna 18s Repetitive Dna Tropomyosin Cell Nucleus Antigen Nucleolar Organizer Region Associated Proteins Ribosome Dna Rna 18s Tropomyosin Acrocentric Chromosome C Banding Chromosome Nor Chromosome Number Cytogenetics Diploidy Female In Situ Hybridization, Fluorescence Heterochromatin Karyotype Male Nonhuman Priority Journal Puffer Fish Sex Chromosome Telomere X Chromosome Y Chromosome Animals Brasil Chromosome Banding Pattern Gene Amplification Genetics Nucleotide Repeat Sex Chromosome Sex Determination Processes Tetraodontiformes Ultrastructure Animal Antigens, Nuclear Brasil Chromosome Banding Ribosomal Dna Female Gene Amplification In Situ Hybridization, Fluorescence Male Repetitive Sequences, Nucleic Acid Rna, Ribosomal, 18s Sex Chromosomes Sex Determination Processes Telomere Tetraodontiformes Tropomyosin |
| description |
The genus Colomesus is the sole representative of the family Tetraodontidae in the Amazon region. Here, Colomesus asellus was analyzed using conventional and molecular cytogenetic protocols. Its diploid chromosome number is 2n = 46 with 12 meta-, 10 submeta-, 16 subtelo-, and 8 acrocentric chromosomes and a fundamental number of FN = 84. An XX/XY sex chromosome system was identified. Mapping of 18S rDNA correlated with the nucleolus organizer regions (Ag-NORs) in the short arms of the 2 X chromosomes in females and in the Y chromosome in males. C-banding revealed heterochromatin in the centromeric regions of all chromosomes, except for pair 3. Prominent sex chromosome-specific heterochromatin amplification was observed, covering the short arms of the Y chromosome almost entirely. FISH with telomeric and tropomyosin (tpm1) sequences, respectively, revealed terminal signals in all chromosomes. The analysis of extended DNA fibers confirmed the colocalization and the interspersed pattern of the telomeric and tpm1 sequences. Thus, this study highlights the remarkable evolutionary dynamism presented by the Amazonian puffer fish regarding the differentiation of a heteromorphic XY sex chromosome system and a particular sex-specific amplification of rDNA sites. This is the first record of such an association in the Tetraodontidae family. © 2018 S. Karger AG, Basel. |
| format |
Artigo |
| author |
Viana, Patrik Ferreira |
| author2 |
Ezaz, Tariq Tariq Marajó, Leandro Ferreira, Milena Zuanon, Jansen Cioffi, Marcelo de Bello Bertollo, Luiz Antônio Carlos Gross, Maria Claudia Feldberg, Eliana |
| author2Str |
Ezaz, Tariq Tariq Marajó, Leandro Ferreira, Milena Zuanon, Jansen Cioffi, Marcelo de Bello Bertollo, Luiz Antônio Carlos Gross, Maria Claudia Feldberg, Eliana |
| title |
Genomic Organization of Repetitive DNAs and Differentiation of an XX/XY Sex Chromosome System in the Amazonian Puffer Fish, Colomesus asellus (Tetraodontiformes) |
| title_short |
Genomic Organization of Repetitive DNAs and Differentiation of an XX/XY Sex Chromosome System in the Amazonian Puffer Fish, Colomesus asellus (Tetraodontiformes) |
| title_full |
Genomic Organization of Repetitive DNAs and Differentiation of an XX/XY Sex Chromosome System in the Amazonian Puffer Fish, Colomesus asellus (Tetraodontiformes) |
| title_fullStr |
Genomic Organization of Repetitive DNAs and Differentiation of an XX/XY Sex Chromosome System in the Amazonian Puffer Fish, Colomesus asellus (Tetraodontiformes) |
| title_full_unstemmed |
Genomic Organization of Repetitive DNAs and Differentiation of an XX/XY Sex Chromosome System in the Amazonian Puffer Fish, Colomesus asellus (Tetraodontiformes) |
| title_sort |
genomic organization of repetitive dnas and differentiation of an xx/xy sex chromosome system in the amazonian puffer fish, colomesus asellus (tetraodontiformes) |
| publisher |
Cytogenetic and Genome Research |
| publishDate |
2020 |
| url |
https://repositorio.inpa.gov.br/handle/1/16988 |
| _version_ |
1787143554322137088 |
| score |
11.674752 |