Bacterial resistance is a global health problem, where Escherichia coli (E. coli) is
often involved in multidrug resistance to clinically important antibiotics for treatment in
humans, and this bacterial species is often used as an indicator of resistance in
bacterial products. animal origin, as this is one of the main routes of spread of
antibiotic resistance to humans according to the World Health Organization (WHO).
In addition, cattle are known to be asymptomatic reservoirs and potential
disseminators of O157: H7, a serotype of worldwide importance for triggering severe
renal and neurological complications in humans. Similarly, E. coli enteropathogens
serogroup also stands out as being a major cause of childhood diarrhea worldwide,
however, there is little epidemiological data on its frequency in cattle intended for
human consumption. Thus, the aim of the present study was to determine the
susceptibility to antibiotics routinely used in health care by means of disc-diffusion
microbial susceptibility testing, as well as to identify O157: H7, EPEC (A, B and C)
and EIEC seropathotypes. (A and B) and search for the eae and rfb O157: H7 gene
in 259 fecal isolated E. coli samples and ruminal content of 323 cattle slaughtered
under State Inspection in Araguaína-To. In total, 259 isolates of E. coli were
obtained, 186 isolates of feces and 73 isolates of ruminal content. Regarding
serology, 149 of the isolates were positive against O157: H7 and EPEC antisera, 22
for O157: H7 and 127 for EPEC (A, B and C), there was a higher positive rate for
serogroup B. 110 negative samples were obtained from the antisera. And as for gene
research, 7 samples out of 259 were positive for the eae gene and none for the rfb
O157: H7 gene. Overall, sulfonamide was the antibiotic with the highest resistance,
followed by florfenicol, gentamicin, enrofloxacin and amoxicillin + clavulanic acid,
doxycycline and cefepime. By analyzing the different biological materials
investigated, stool isolates were resistant to four antibiotics and those of rumen
content to eight, and when comparing the multidrug resistance profile stool isolates
were resistant to ciprofloxacin and those of rumen content to chloramphenicol,
enrofloxacin, cefepime and doxycycline. O157: H7 antiserum positive isolates were
multidrug resistant to 9 antibiotics, and EPEC antisera positive isolates (A, B and C)
were multidrug resistant to 2 antibiotics. The isolates positive for the eae gene
showed multidrug resistance to 6 antibiotics. In our study, negative samples against
the antisera tested showed multidrug resistance profile to 5 antibiotics. The multidrug
resistance profile found in this study suggests the need for greater caution in the use
of antibiotics for cattle in the region studied, given the high resistance rates obtained
and the concomitant use of some clinically important antibiotics in humans, and the
technique of Direct agglutination used suggest cattle as a potential reservoir of the
EPEC category.
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