The biodegradation of lignocellulosic materials has been the object of many
studies being applied in technological processes. The technology to convert
lignocellulosic biomass into fermentable sugars presents great sustainable
potential for the production of second generation ethanol. Obtaining ethanol
from biomass involves two steps, first consisting of the hydrolysis of
polysaccharides, generating mono and disaccharides, and later involves the
fermentation of monkeys and disaccharides in ethanol. Cellulose hydrolysis
generates glucose and cellobiose (a glucose dimer). On the other hand, the
hydrolysis of lignins and hemicellulose generates sugars and by-products
(mainly HMF, furfural and acetic acid), which often inhibit microbial
fermentation. This work aimed to optimize the enzymatic hydrolysis of the
potato branch to obtain second generation ethanol. For the development of the
experiment, a rotational central composite planning was performed, varying
temperature (30 ° C, 40 ° C and 50 ° C), time (24, 48 and 72 hours) and
enzymatic loading (30, 120 and 210FPU). Response variables were the
concentrations of monomers (glucose, xylose and arabinose) and inhibitors
(acetic acid, hydroxymethylfurfural and furfural) in the hydrolyzate. The optimal
operating conditions for the experiment were defined as temperature of 30 ° C,
process time of 72 hours, enzymatic loading of 30FPU, under these conditions
we would have 11.97 g / L glucose, 2.29 g / L xylose. The effects of the factors
caused low concentrations of furfural and HMF inhibitors without affecting the
yield of the process.
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