Enzimas lignocelulolíticas de basidiomicetos cultivados em biomassas vegetais oriundas da agroindústria do dendê e obtenção de açúcares fermentescíveis
Three vegetable residues from the palm oil industry were used as substrate for cultivation of different white rot fungi strains (basidiomycetes). The growth and production of lignocellulolytic enzymes were evaluated by solid and submerged fermentation. The enzymatic extracts obtained from ferment...
| Autor principal: | Peláez, Rubén Darío Romero |
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| Grau: | Dissertação |
| Idioma: | pt_BR |
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Universidade Federal do Tocantins
2017
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| Acesso em linha: |
http://hdl.handle.net/11612/501 |
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ir-11612-501 |
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ir-11612-5012019-05-25T06:12:23Z Enzimas lignocelulolíticas de basidiomicetos cultivados em biomassas vegetais oriundas da agroindústria do dendê e obtenção de açúcares fermentescíveis Peláez, Rubén Darío Romero Siqueira, Félix Gonçalves de Mendonça, Simone Pré-tratamento biológico Cocultivos Lacases Planejamento experimental simplex lattice Biological pretreatment Cocultures Laccases Simplex lattice experimental design CNPQ::ENGENHARIAS Three vegetable residues from the palm oil industry were used as substrate for cultivation of different white rot fungi strains (basidiomycetes). The growth and production of lignocellulolytic enzymes were evaluated by solid and submerged fermentation. The enzymatic extracts obtained from fermentations were used in enzymatic hydrolysis process of pretreated sugarcane bagasse and oil palm empty bunch. Fifty-four macrofungal strains were cultivated in three formulations of oil palm biomass on Petri dishes, where 5 strains with faster and dense growth were chosen. These five macrofungal strains were cultivated in solid state fermentation and evaluated by the enzymatic activities of the extracts obtained. In these extracts, activities of laccase, peroxidase, manganese peroxidase and protease were found to be predominant. Three of the five strains were selected with predominance in oxidative activities on solid state fermentation cultures to be evaluated in submerged fermentation (monocultures) using synthetic medium supplemented with oil palm biomass. The enzymatic activities of the three basidiomycetes srtains were compared with five fungal strains frequently used in the literature. There were significant differences in function of the oxidative activities (laccase and peroxidases) and hydrolytic activities (PFase and β-glucosidases) among the tested strains. These differences were used to establish subgroups which were evaluated through the interaction in plates and cocultures in submerged fermentation. The enzymatic activities of the extracts obtained from monocultures and cocultures presented differences, with predominantly positive interactions between the three basidiomycetes and T. reesei ATCC® 60787. Monocultures and cocultures were compared as a function of the glucose release after the enzymatic hydrolysis of sugarcane bagasse pretreated by autohydrolysis. The enzymatic hydrolysis of the coculture extracts obtained higher percentages (maximum value 44.7%) when compared to monocultures. An analysis of simplex lattice mixtures was made using the monoculture extracts as components to obtain an enzymatic cocktail in order to optimize the glucose release of the pretreated sugarcane bagasse. The results showed that extracts with higher hydrolytic activities are correlated with the higher glucose release and extracts with oxidative enzymes can improve the sugar yield, thus having a mixture or cocktail characterized by hydrolytic and oxidative enzymes with the potential to obtain sugars. Finally, this cocktail was used in the hydrolysis of untreated, physically, biologically and biologically-physically (combined) pretreated oil palm empty bunch, obtaining a maximum yield of 11.8 gL-1 of glucose in the biomass of palm oil when pretreated biological-physically, which corresponded between 40-60% of the yield of the commercial enzymes Cellic® Ctec3 and Cellic® Ctec2. Três resíduos vegetais da agroindústria do dendê foram usados como substrato para o cultivo de distintas linhagens de fungos da podridão-branca (basidiomicetos). Foram avaliados o crescimento e produção de enzimas lignocelulolíticas por fermentação em estado sólido e submerso. Os extratos enzimáticos obtidos das fermentações foram utilizados no processo de hidrólise enzimática de bagaço de cana-de-açúcar e cacho vazio do dendê previamente pré-tratados. Um total de 54 linhagens de macrofungos foram cultivadas em três formulações de biomassa de dendê em placas Petri, onde foram escolhidas 5 linhagens com crescimento mais rápido e denso. Estas cinco linhagens de macrofungos foram cultivadas em fermentação em estado sólido e avaliados pelas atividades enzimáticas dos extratos obtidos. Nestes extratos, houve predominância das atividades de lacase, peroxidase, manganês peroxidase e protease. Das cinco linhagens foram selecionadas três linhagens com predominância nas atividades oxidativas nos cultivos em fermentação em estado sólido, para serem avaliadas em fermentação submersa (monocultivos) usando meio sintético suplementado com biomassa vegetal do dendê. As atividades enzimáticas das três espécies de basidiomicetos foram comparadas com cinco linhagens fúngicas usadas frequentemente na literatura. Houve diferenças significativas em função das atividades oxidativas (lacase e peroxidases) e hidrolíticas (FPase e β-glicosidases) entre as linhagens testadas. Estas diferenças foram usadas para estabelecer subgrupos, os quais foram avaliados através da interação em placa e cocultivos em fermentação submersa. As atividades enzimáticas dos extratos obtidos de monocultivos e cocultivos apresentaram diferenças com interações predominantemente positivas entre os três basidiomicetos e T. reesei ATCC® 60787. Os monocultivos e cocultivos foram comparados em função da liberação de glicose após da hidrólise enzimática de bagaço de cana-de-açúcar pré-tratado por autohidrólise. As hidrólises enzimáticas aplicando os extratos dos cocultivos obtiveram rendimento 44,7% superior quando comparadas com os monocultivos. Foi feita uma análise de misturas simplex lattice, usando como componentes os extratos dos monocultivos para a obtenção de um coquetel enzimático a fim de otimizar a liberação de glicose do bagaço de cana pré-tratado. Os resultados demostraram que os extratos com maiores atividades hidrolíticas estão correlacionadas com a maior liberação de glicose, e que os extratos com enzimas oxidativas podem melhorar o rendimento, tendo assim uma mistura ou coquetel caracterizado com enzimas hidrolíticas e oxidativas com potencial para obtenção de açúcares. Finalmente, este coquetel foi utilizado na hidrólise de cacho vazio de dendê pré-tratado fisicamente, biologicamente e biológica-físicamente (combinado) obtendo um rendimento máximo de 11,8 g.L-1 de glicose a partir de biomassa vegetal de dendê quando pré-tratada biológicafisicamente, o que correspondeu entre 40 - 60% do rendimento do rendimento obtido quando empregadas as enzimas comerciais Cellic® Ctec3 e Cellic® Ctec2. 2017-09-14T13:22:58Z 2017-09-14T13:22:58Z 2017-05-31 Dissertação PELÁEZ, Rubén Darío Romero.Enzimas lignocelulolíticas de basidiomicetos cultivados em biomassas vegetais oriundas da agroindústria do dendê e obtenção de açúcares fermentescíveis.2017.145f. Dissertação (Mestrado em Biotecnologia) – Universidade Federal do Tocantins, Programa de Pós-Graduação em Biotecnologia, Gurupi, 2017. http://hdl.handle.net/11612/501 pt_BR Open Access application/pdf application/pdf Universidade Federal do Tocantins BR Programa de Pós-Graduação em Biotecnologia - PPGB Gurupi |
| institution |
Repositório Institucional - Universidade Federal do Tocantins - UFT |
| collection |
RepositorioUFT |
| language |
pt_BR |
| topic |
Pré-tratamento biológico Cocultivos Lacases Planejamento experimental simplex lattice Biological pretreatment Cocultures Laccases Simplex lattice experimental design CNPQ::ENGENHARIAS |
| spellingShingle |
Pré-tratamento biológico Cocultivos Lacases Planejamento experimental simplex lattice Biological pretreatment Cocultures Laccases Simplex lattice experimental design CNPQ::ENGENHARIAS Peláez, Rubén Darío Romero Enzimas lignocelulolíticas de basidiomicetos cultivados em biomassas vegetais oriundas da agroindústria do dendê e obtenção de açúcares fermentescíveis |
| topic_facet |
Pré-tratamento biológico Cocultivos Lacases Planejamento experimental simplex lattice Biological pretreatment Cocultures Laccases Simplex lattice experimental design CNPQ::ENGENHARIAS |
| description |
Three vegetable residues from the palm oil industry were used as substrate for
cultivation of different white rot fungi strains (basidiomycetes). The growth and
production of lignocellulolytic enzymes were evaluated by solid and submerged
fermentation. The enzymatic extracts obtained from fermentations were used in
enzymatic hydrolysis process of pretreated sugarcane bagasse and oil palm empty
bunch. Fifty-four macrofungal strains were cultivated in three formulations of oil palm
biomass on Petri dishes, where 5 strains with faster and dense growth were chosen.
These five macrofungal strains were cultivated in solid state fermentation and
evaluated by the enzymatic activities of the extracts obtained. In these extracts,
activities of laccase, peroxidase, manganese peroxidase and protease were found to
be predominant. Three of the five strains were selected with predominance in oxidative
activities on solid state fermentation cultures to be evaluated in submerged
fermentation (monocultures) using synthetic medium supplemented with oil palm
biomass. The enzymatic activities of the three basidiomycetes srtains were compared
with five fungal strains frequently used in the literature. There were significant
differences in function of the oxidative activities (laccase and peroxidases) and
hydrolytic activities (PFase and β-glucosidases) among the tested strains. These
differences were used to establish subgroups which were evaluated through the
interaction in plates and cocultures in submerged fermentation. The enzymatic
activities of the extracts obtained from monocultures and cocultures presented
differences, with predominantly positive interactions between the three basidiomycetes
and T. reesei ATCC® 60787. Monocultures and cocultures were compared as a
function of the glucose release after the enzymatic hydrolysis of sugarcane bagasse
pretreated by autohydrolysis. The enzymatic hydrolysis of the coculture extracts
obtained higher percentages (maximum value 44.7%) when compared to
monocultures. An analysis of simplex lattice mixtures was made using the monoculture
extracts as components to obtain an enzymatic cocktail in order to optimize the glucose
release of the pretreated sugarcane bagasse. The results showed that extracts with
higher hydrolytic activities are correlated with the higher glucose release and extracts
with oxidative enzymes can improve the sugar yield, thus having a mixture or cocktail
characterized by hydrolytic and oxidative enzymes with the potential to obtain sugars. Finally, this cocktail was used in the hydrolysis of untreated, physically, biologically and
biologically-physically (combined) pretreated oil palm empty bunch, obtaining a
maximum yield of 11.8 gL-1 of glucose in the biomass of palm oil when pretreated
biological-physically, which corresponded between 40-60% of the yield of the
commercial enzymes Cellic® Ctec3 and Cellic® Ctec2. |
| author_additional |
Siqueira, Félix Gonçalves de |
| author_additionalStr |
Siqueira, Félix Gonçalves de |
| format |
Dissertação |
| author |
Peláez, Rubén Darío Romero |
| title |
Enzimas lignocelulolíticas de basidiomicetos cultivados em biomassas vegetais oriundas da agroindústria do dendê e obtenção de açúcares fermentescíveis |
| title_short |
Enzimas lignocelulolíticas de basidiomicetos cultivados em biomassas vegetais oriundas da agroindústria do dendê e obtenção de açúcares fermentescíveis |
| title_full |
Enzimas lignocelulolíticas de basidiomicetos cultivados em biomassas vegetais oriundas da agroindústria do dendê e obtenção de açúcares fermentescíveis |
| title_fullStr |
Enzimas lignocelulolíticas de basidiomicetos cultivados em biomassas vegetais oriundas da agroindústria do dendê e obtenção de açúcares fermentescíveis |
| title_full_unstemmed |
Enzimas lignocelulolíticas de basidiomicetos cultivados em biomassas vegetais oriundas da agroindústria do dendê e obtenção de açúcares fermentescíveis |
| title_sort |
enzimas lignocelulolíticas de basidiomicetos cultivados em biomassas vegetais oriundas da agroindústria do dendê e obtenção de açúcares fermentescíveis |
| publisher |
Universidade Federal do Tocantins |
| publishDate |
2017 |
| url |
http://hdl.handle.net/11612/501 |
| _version_ |
1787134776228970496 |
| score |
11.755432 |