The production of biodiesel has been accompanied by the increase of by-products, among which
crude glycerin, which has been the subject of numerous researches in which its use as an energy
source for feed of production animals is highlighted. The present study aimed to establish the
values of zootechnical performance, body composition, hematological parameters, biochemical
parameters and histopathological analysis of juveniles of "Painted-of-Amazonia" after feeding
with rations containing increasing levels of crude glycerin. The experiment was carried out at
theNeotropical Fish Morphophysiology and Biochemistry Laboratory of the Federal University
of Tocantins, 150 juveniles of Amazonian Paints, with initial weight of 6.83 ± 1.11 (g) and
length of 10, 06 ± 0.57 cm distributed in a completely randomized design (DIC), with 3
replicates (10 animals in each replicate). The animals were fed five diets with increasing levels
of glycerol (0%, 5%, 7.5%, 10% and 12.5%) and fed for a period of 90 days (30 days of
adaptation and 60 experimental days). At the end of this period, the animals were used to
measure their performance (length and final weight, survival, specific growth
rate, hepatosomatic index, condition factor, weight gain, conversion and feed efficiency). de insensitized in the ice to perform the analysis of fish body composition, according to the
standard methodology described by INCT (2012). Hematological parameters were determined:
hematocrit, hemoglobin, erythrocyte count, and hematocrit indices: VCM (mean corpuscular
volume), HCM (mean corpuscular hemoglobin) and CHCM (mean corpuscular hemoglobin
concentration) and for biochemical analysis: total protein concentration, total cholesterol,
triglycerides, Aspartate Amino Transferase (AST) and Enzyme Alanine Amino Transferase
(ALT), hepatic, muscle glycogen and blood glucose. Histopathological analyzes of the liver
and kidney of the animals were also performed. The specific growth rate showed moderate
linear behavior and muscle glycogen at the level of 12.5% presented a lower concentration in
relation to the control diet (0.0%). As for histology, crude glycerin did not cause significant
hepatic and renal changes in the studied species, since the alterations found in the two tissues
were considered lesions that did not compromise the functioning of the organ or reversible
lesions. Finally, the juveniles of Painted Amazonian are able to metabolize the crude glycerin
up to the level of 10.0%. Since, until this level, no significant changes were observed in the
zootechnical, body composition, hematological, biochemical and histological parameters of the
animal.
|
