Estudo in vitro do potencial antagônico de isolados de Trichoderma spp. ao fitopatógeno Fusarium oxysporum
The phytopathogen Fusarium oxysporum, belonging to the genus that causes the disease popularly known as fusariosis or Fusarium wilt, affects several agricultural crops worldwide. Its high degree of pathogenicity is associated with its ability to colonize the vascular system of plants, as well as...
| Autor principal: | Alencar, Kamila Lourrane Carvalho |
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| Grau: | Monografia |
| Idioma: | pt_BR |
| Publicado em: |
Universidade Federal do Tocantins
2023
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| Acesso em linha: |
http://hdl.handle.net/11612/6238 |
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ir-11612-6238 |
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ir-11612-62382023-12-22T06:03:54Z Estudo in vitro do potencial antagônico de isolados de Trichoderma spp. ao fitopatógeno Fusarium oxysporum Alencar, Kamila Lourrane Carvalho Lopes, Fabyano Alvares Cardoso Controle biológico Ensaio enzimático Micoparasitismo Murcha de fusário Biological control Enzyme assay Mycoparasitism Fusarium wilt CNPQ::CIENCIAS BIOLOGICAS The phytopathogen Fusarium oxysporum, belonging to the genus that causes the disease popularly known as fusariosis or Fusarium wilt, affects several agricultural crops worldwide. Its high degree of pathogenicity is associated with its ability to colonize the vascular system of plants, as well as the production of resistance structures in the soil, the chlamydospores. Considering the need for methods that contribute to agricultural development without causing damage to the environment and the population, biological control agents (BCAs) are now seen as an excellent artifice. Fungi of the genus Trichoderma can be considered cunning BCAs because they have numerous mechanisms with high antagonistic potential that impair spore germination, hyphal growth and chlamydospore development of various phytopathogens. The present work sought to evaluate the efficiency of Trichoderma spp. in the biocontrol of the phytopathogen F. oxysporum through pairing tests, enzymatic analysis, and production of secondary metabolites. All isolates had results considered satisfactory, according to the Bell scale, in the pairing test. However, in the volatile metabolites test, none of the isolates achieved an inhibitory effect. The analysis of non-volatile metabolites showed that the isolate of T. asperelloides TR-356 can inhibit the mycelial growth of F. oxysporum without the need for direct contact between the hyphae. The enzymatic tests indicated that the isolates of Trichoderma spp., when in the presence of macerated mycelia of the phytopathogen F. oxysporum, showed hydrolytic activities of important compounds present in the cell wall of the host, with emphasis in this action on the isolates T. asperelloides TR-356 and Trichoderma spp. 2. With the results obtained, it was possible to conclude that the isolates T. asperelloides TR- 356 and Trichoderma spp. 2 were the most effective in antagonizing the phytopathogen F. oxysporum, making them potential BCAs. O fitopatógeno Fusarium oxysporum, pertencente ao gênero causador da doença conhecida popularmente como fusariose ou murcha do fusário, acomete diversas culturas agrícolas em todo o mundo. Seu alto grau de patogenicidade está associado à sua capacidade de colonização do sistema vascular das plantas, como também, pela produção de estruturas de resistências no solo, os clamidósporos. Considerando a necessidade de métodos que contribuam no desenvolvimento agrícola sem promover danos ao ambiente e a população, os agentes de controle biológico (ACBs) são hoje vistos como um excelente artifício. Fungos do gênero Trichoderma podem ser considerados ACBs sagazes por apresentarem inúmeros mecanismos com alto potencial antagônico que prejudicam a germinação de esporos, o crescimento das hifas e o desenvolvimento de clamidósporo de diversos fitopatógenos. O presente trabalho buscou avaliar a eficiência de isolados de Trichoderma spp. no biocontrole do fitopatógeno F. oxysporum por meio de testes de pareamento, análise enzimática e produção de metabólitos secundários. Todos os isolados tiveram resultados considerados satisfatórios, segundo a escala de Bell, no teste de pareamento. Entretanto, no teste de metabólitos voláteis nenhum isolado alcançou efeito inibitório. Já a análise dos metabólitos não voláteis mostrou que o isolado de T. asperelloides TR-356 pode inibir o crescimento micelial do F. oxysporum sem a necessidade do contato direto entre as hifas. Os testes enzimáticos indicaram que os isolados de Trichoderma spp., quando em presença de micélios macerados do fitopatógeno F. oxysporum apresentaram atividades hidrolíticas de importantes compostos presentes na parede celular do hospedeiro, tendo destaque nessa ação os isolados T. asperelloides TR-356 e Trichoderma spp. 2. Com os resultados obtidos, foi possível concluir que os isolados T. asperelloides TR-356 e Trichoderma spp. 2 foram os mais eficazes no antagonismo ao fitopatógeno F. oxysporum, tornando-os potenciais ACBs. 2023-12-21T12:56:05Z 2023-12-21T12:56:05Z 2023-12-21 Monografia ALENCAR, Kamila Lourrane Carvalho. Estudo in vitro do potencial antagônico de isolados de Trichoderma spp. ao fitopatógeno Fusarium oxysporum. 2022. 48 f. TCC (Graduação) - Curso de Ciências Biológicas, Universidade Federal do Tocantins, Porto Nacional, 2023. http://hdl.handle.net/11612/6238 pt_BR ACESSO LIVRE application/pdf Universidade Federal do Tocantins Porto Nacional CURSO::PORTO NACIONAL::PRESENCIAL::BACHARELADO::CIÊNCIAS BIOLÓGICAS Porto Nacional Graduação |
| institution |
Repositório Institucional - Universidade Federal do Tocantins - UFT |
| collection |
RepositorioUFT |
| language |
pt_BR |
| topic |
Controle biológico Ensaio enzimático Micoparasitismo Murcha de fusário Biological control Enzyme assay Mycoparasitism Fusarium wilt CNPQ::CIENCIAS BIOLOGICAS |
| spellingShingle |
Controle biológico Ensaio enzimático Micoparasitismo Murcha de fusário Biological control Enzyme assay Mycoparasitism Fusarium wilt CNPQ::CIENCIAS BIOLOGICAS Alencar, Kamila Lourrane Carvalho Estudo in vitro do potencial antagônico de isolados de Trichoderma spp. ao fitopatógeno Fusarium oxysporum |
| topic_facet |
Controle biológico Ensaio enzimático Micoparasitismo Murcha de fusário Biological control Enzyme assay Mycoparasitism Fusarium wilt CNPQ::CIENCIAS BIOLOGICAS |
| description |
The phytopathogen Fusarium oxysporum, belonging to the genus that causes the disease
popularly known as fusariosis or Fusarium wilt, affects several agricultural crops worldwide.
Its high degree of pathogenicity is associated with its ability to colonize the vascular system of
plants, as well as the production of resistance structures in the soil, the chlamydospores.
Considering the need for methods that contribute to agricultural development without causing
damage to the environment and the population, biological control agents (BCAs) are now seen
as an excellent artifice. Fungi of the genus Trichoderma can be considered cunning BCAs
because they have numerous mechanisms with high antagonistic potential that impair spore
germination, hyphal growth and chlamydospore development of various phytopathogens. The
present work sought to evaluate the efficiency of Trichoderma spp. in the biocontrol of the
phytopathogen F. oxysporum through pairing tests, enzymatic analysis, and production of
secondary metabolites. All isolates had results considered satisfactory, according to the Bell
scale, in the pairing test. However, in the volatile metabolites test, none of the isolates achieved
an inhibitory effect. The analysis of non-volatile metabolites showed that the isolate of T.
asperelloides TR-356 can inhibit the mycelial growth of F. oxysporum without the need for
direct contact between the hyphae. The enzymatic tests indicated that the isolates of
Trichoderma spp., when in the presence of macerated mycelia of the phytopathogen F.
oxysporum, showed hydrolytic activities of important compounds present in the cell wall of the
host, with emphasis in this action on the isolates T. asperelloides TR-356 and Trichoderma spp.
2. With the results obtained, it was possible to conclude that the isolates T. asperelloides TR-
356 and Trichoderma spp. 2 were the most effective in antagonizing the phytopathogen F.
oxysporum, making them potential BCAs. |
| author_additional |
Lopes, Fabyano Alvares Cardoso |
| author_additionalStr |
Lopes, Fabyano Alvares Cardoso |
| format |
Monografia |
| author |
Alencar, Kamila Lourrane Carvalho |
| title |
Estudo in vitro do potencial antagônico de isolados de Trichoderma spp. ao fitopatógeno Fusarium oxysporum |
| title_short |
Estudo in vitro do potencial antagônico de isolados de Trichoderma spp. ao fitopatógeno Fusarium oxysporum |
| title_full |
Estudo in vitro do potencial antagônico de isolados de Trichoderma spp. ao fitopatógeno Fusarium oxysporum |
| title_fullStr |
Estudo in vitro do potencial antagônico de isolados de Trichoderma spp. ao fitopatógeno Fusarium oxysporum |
| title_full_unstemmed |
Estudo in vitro do potencial antagônico de isolados de Trichoderma spp. ao fitopatógeno Fusarium oxysporum |
| title_sort |
estudo in vitro do potencial antagônico de isolados de trichoderma spp. ao fitopatógeno fusarium oxysporum |
| publisher |
Universidade Federal do Tocantins |
| publishDate |
2023 |
| url |
http://hdl.handle.net/11612/6238 |
| _version_ |
1787134556559638528 |
| score |
11.675088 |