The study of secondary metabolites produced by fungi has aroused the interest of
researchers who want to find new sources of compounds that may contribute to the development
of industry, agriculture or health. The present study evaluated the chemical profile by TLC and
HPLC of crude extracts of the acetonitrile and hexane fractions obtained from the cultivation
of the endophytic fungus Coriolopsis rigida, isolated from Cochlospermum regium, in rice solid
medium, which showed a variety of compounds. Using the same techniques, the chemical
profile of the crude ACN extract obtained from cultivation in rice was compared with the
chemical profile of the crude extract of the ethyl acetate obtained from cultivation in Czapek
liquid medium. From analyzes it was observed that the extract obtained from the fungus grown
in rice signaled a greater diversity of compounds than the extract obtained from the fungus
grown in Czapek. The crude ACN extract was subjected to fractionation using chromatographic
techniques resulting in two isolated substances, tyrosol (1) and 1-N-methyl-p-phenol
acetamide-2-O-glycoside-propan-1-one (2), which had their structures elucidated through
NMR analyses. The allelopathy and toxicity assay of the crude extract ACN indicated the
inhibition of the germination of the lettuce seeds and it was configured as non-toxic for the
microcrustacean Artemia sp. The volatile organic compounds of the endophilic fungus
cultivated in solid rice medium, obtained by hydrodistillation, were analyzed by GC-MS,
resulting in 16 identified compounds and having as majority the 5-methyl-2-
furanocarboxaldehyde, semi-quantified with 29.24% and 620.05 μg. Volatile compounds
produced by the fungus grown in PDA medium using solid phase microextraction were also
analyzed by GC-MS, 5 compounds were identified and the majority compound was semi-
quantified phenylethyl alcohol with 49.17% and 935.43 ng. The allelochemical assay of the
volatile organic compounds of the fungus was evaluated on the germination of lettuce seeds,
showing low inhibition, reducing the average root lengths by 18% and 8% in the average length
of the seedlings. The fungus C. rigida presents itself as an important object of research since
its metabolic production provides a variety of substances to be investigated.
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