The aquatic environment is one the most impacted environment due to human pressure. The agriculture practice, strongly dependent of agrotoxic compounds, has shown as important source of environmental contamination. The use of fishes as sentinel species is a common procedure for investigation of genotoxic potential of aquatic pollutants. In the present study we analyze the mutagenic and genotoxic potential of the herbicides Imazetapir and Glifosato on blood cells of tambaqui, Colossoma macropomum. Juvenile tambaqui were exposed to three sub lethal concentrations of Glifosato: C1 (1/50 CL50 = 1,86 mg.L-1), C2 (1/100 CL50 = 0,93 mg.L-1) e C3 (1/1000 CL50 = 0,093 mg.L-1). The toxic effects of these molecules had been availed based on acute toxicity test (CL50-96hs) and micronucleus and comet assays.
The CL50-96hs of Imazetapir was observed at concentration of 185 mg.L-1, while Glifosato was 92,90 mg.L-1. The treatment C1 showed increasing of micronucleated cells after seven days exposure and DNA damage after five to seven days from the experiment starting. Both herbicides were classified as little toxic but presents potential risk to aquatic life.
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