The copaiba oleoresin has several proven medicinal properties, among them the antimicrobial
activity. The aim of this work was to evaluate the chemical composition of Copaifera
reticulata Ducke oleoresin, cytotoxicity and antimicrobial activity against Staphylococcus
aureus strains; Methicillin-resistant S. aureus and S. simulans in the seasonal (dry and rainy)
periods of oleoresin, as well as obtain a semi-solid herbal formulation containing Copaifera
reticulata Ducke oleoresin. For this, Gas Chromatography with Mass Spectrometry (GC-MS)
was performed to evaluate the chemical composition of the oleoresin, cytotoxic activity in
vitro using VERO cells. Microbiological activity by well diffusion and plate microdilution
and the formulation was obtained by melting the murumuru butter at 45 ° C and then with the
addition of surfactant, water and oil to form an emulsified system. The results showed that it
was possible to identify only three samples, being described as Sample 1 of dry and rainy
period (1S and 1C) Sample 2 of dry and rainy period (2S and 2C) and Sample 3 of dry and
rainy period (3S and 3C) botanically, in the chemical analysis β-caryophyllene was identified
as the major component in all samples used, samples 2 C, 2 S, 3 C and 3 S showed
cytotoxicity only at 200 µg / mL, and the sample 1 C and 1 S showed no cytotoxicity in any
of the concentrations used, in the bacteria used it found antibacterial action indicating that
there was action of oleoresin. The determination of the Minimum Inhibitory Concentration
(MIC) showed that the oleoresin collected in the rainy season showed strong inhibition for S.
aureus methicillin resistant bacteria and S. aueures, while in the dry period showed a weak
inhibition for all strains tested. The formulation incorporated with oleoresin was stable, gel like, light yellow in color and woody odor, with the appearance of anisotropy and
birefringence, with streak-shaped texture, characteristic of hexagonal phase that has been used
in some studies for sustained release of drugs. The results obtained in the present study
further reinforce the importance of copaiba oleoresin from the Amazon region as a
phytotherapic with antibacterial potential, thus being a promising source for the synthesis of
new drugs with antimicrobial activity.
|
