Development and characterization of a packaging cell line for pseudo-infectious yellow fever virus particle generation
Introduction: Pseudo-infectious yellow fever viral particles (YFV-PIVs) have been used to study vaccines and viral packaging. Here, we report the development of a packaging cell line, which expresses the YFV prM/E proteins. Methods: HEK293 cells were transfected with YFV prM/E and C (84 nt) genes to...
| Autor principal: | QUEIROZ, Sabrina Ribeiro de Almeida |
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| Outros Autores: | SILVA JÚNIOR, José Valter Joaquim, SILVA, Andrea Nazare Monteiro Rangel da, OLIVEIRA, Amanda Gomes de, SANTOS, Jefferson José da Silva, GIL, Laura Helena Vega Gonzales |
| Grau: | Artigo |
| Idioma: | eng |
| Publicado em: |
Universidade Federal do Pará
2018
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| Acesso em linha: |
http://repositorio.ufpa.br/jspui/handle/2011/10032 http://dx.doi.org/10.1590/0037-8682-0220-2017 |
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ir-2011-10032 |
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ir-2011-100322018-06-15T05:34:16Z Development and characterization of a packaging cell line for pseudo-infectious yellow fever virus particle generation QUEIROZ, Sabrina Ribeiro de Almeida SILVA JÚNIOR, José Valter Joaquim SILVA, Andrea Nazare Monteiro Rangel da OLIVEIRA, Amanda Gomes de SANTOS, Jefferson José da Silva GIL, Laura Helena Vega Gonzales Vírus da febre amarela Infecção viral Partículas pseudo-infecciosas Flavivírus CNPQ::CIENCIAS BIOLOGICAS::MICROBIOLOGIA::BIOLOGIA E FISIOLOGIA DOS MICROORGANISMOS::VIROLOGIA Introduction: Pseudo-infectious yellow fever viral particles (YFV-PIVs) have been used to study vaccines and viral packaging. Here, we report the development of a packaging cell line, which expresses the YFV prM/E proteins. Methods: HEK293 cells were transfected with YFV prM/E and C (84 nt) genes to generate HEK293-YFV-PrM/E-opt. The cells were evaluated for their ability to express the heterologous proteins and to package the replicon repYFV-17D-LucIRES, generating YFV-PIVs. Results: The expression of prM/E proteins was confirmed, and the cell line trans-packaged the replicon for recovery of a reporter for the YFV-PIVs. Conclusions: HEK293-YFV-prM/E-opt trans-packaging capacity demonstrates its possible biotechnology application. 2018-06-14T21:02:08Z 2018-06-14T21:02:08Z 2018-02 Artigo de Periódico QUEIROZ, Sabrina Ribeiro de Almeida et al. Development and characterization of a packaging cell line for pseudo-infectious yellow fever virus particle generation. Revista da Sociedade Brasileira de Medicina Tropical, Uberaba, v. 51, n. 1, p. 66-70, jan./fev. 2018. Disponível em: <http://repositorio.ufpa.br/jspui/handle/2011/10032>. Acesso em:. 0037-8682 1678-9849 http://repositorio.ufpa.br/jspui/handle/2011/10032 http://dx.doi.org/10.1590/0037-8682-0220-2017 eng Revista da Sociedade Brasileira de Medicina Tropical Acesso Aberto application/pdf Universidade Federal do Pará Brasil UFPA http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822018000100066&lng=pt&nrm=iso |
| institution |
Repositório Institucional - Universidade Federal do Pará |
| collection |
RI-UFPA |
| language |
eng |
| topic |
Vírus da febre amarela Infecção viral Partículas pseudo-infecciosas Flavivírus CNPQ::CIENCIAS BIOLOGICAS::MICROBIOLOGIA::BIOLOGIA E FISIOLOGIA DOS MICROORGANISMOS::VIROLOGIA |
| spellingShingle |
Vírus da febre amarela Infecção viral Partículas pseudo-infecciosas Flavivírus CNPQ::CIENCIAS BIOLOGICAS::MICROBIOLOGIA::BIOLOGIA E FISIOLOGIA DOS MICROORGANISMOS::VIROLOGIA QUEIROZ, Sabrina Ribeiro de Almeida Development and characterization of a packaging cell line for pseudo-infectious yellow fever virus particle generation |
| topic_facet |
Vírus da febre amarela Infecção viral Partículas pseudo-infecciosas Flavivírus CNPQ::CIENCIAS BIOLOGICAS::MICROBIOLOGIA::BIOLOGIA E FISIOLOGIA DOS MICROORGANISMOS::VIROLOGIA |
| description |
Introduction: Pseudo-infectious yellow fever viral particles (YFV-PIVs) have been used to study vaccines and viral packaging. Here, we report the development of a packaging cell line, which expresses the YFV prM/E proteins. Methods: HEK293 cells were transfected with YFV prM/E and C (84 nt) genes to generate HEK293-YFV-PrM/E-opt. The cells were evaluated for their ability to express the heterologous proteins and to package the replicon repYFV-17D-LucIRES, generating YFV-PIVs. Results: The expression of prM/E proteins was confirmed, and the cell line trans-packaged the replicon for recovery of a reporter for the YFV-PIVs. Conclusions: HEK293-YFV-prM/E-opt trans-packaging capacity demonstrates its possible biotechnology application. |
| format |
Artigo |
| author |
QUEIROZ, Sabrina Ribeiro de Almeida |
| author2 |
SILVA JÚNIOR, José Valter Joaquim SILVA, Andrea Nazare Monteiro Rangel da OLIVEIRA, Amanda Gomes de SANTOS, Jefferson José da Silva GIL, Laura Helena Vega Gonzales |
| author2Str |
SILVA JÚNIOR, José Valter Joaquim SILVA, Andrea Nazare Monteiro Rangel da OLIVEIRA, Amanda Gomes de SANTOS, Jefferson José da Silva GIL, Laura Helena Vega Gonzales |
| title |
Development and characterization of a packaging cell line for pseudo-infectious yellow fever virus particle generation |
| title_short |
Development and characterization of a packaging cell line for pseudo-infectious yellow fever virus particle generation |
| title_full |
Development and characterization of a packaging cell line for pseudo-infectious yellow fever virus particle generation |
| title_fullStr |
Development and characterization of a packaging cell line for pseudo-infectious yellow fever virus particle generation |
| title_full_unstemmed |
Development and characterization of a packaging cell line for pseudo-infectious yellow fever virus particle generation |
| title_sort |
development and characterization of a packaging cell line for pseudo-infectious yellow fever virus particle generation |
| publisher |
Universidade Federal do Pará |
| publishDate |
2018 |
| url |
http://repositorio.ufpa.br/jspui/handle/2011/10032 http://dx.doi.org/10.1590/0037-8682-0220-2017 |
| _version_ |
1832602267797159936 |
| score |
11.755432 |