O transportador XCG- medeia a captação de glutamato independente de sódio em cultura primária de células gliais da cóclea de camundongos neonatos
The cochlea is a sensory organ of the auditory system whose excitatory synapses are mediated by L-Glutamate. Since glutamate has physiological and pathological repercussions on the cochlea, the crucial role of glutamate transport mechanisms capable of regulating the extracellular concentration...
| Autor principal: | MARTINS, Luana Carvalho |
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| Grau: | Dissertação |
| Idioma: | por |
| Publicado em: |
Universidade Federal do Pará
2023
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| Assuntos: | |
| Acesso em linha: |
http://repositorio.ufpa.br:8080/jspui/handle/2011/15427 |
| Resumo: |
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The cochlea is a sensory organ of the auditory system whose excitatory
synapses are mediated by L-Glutamate. Since glutamate has physiological and
pathological repercussions on the cochlea, the crucial role of glutamate
transport mechanisms capable of regulating the extracellular concentration of
this neurotransmitter in order to maintain auditory function is highlighted. Within
this context, in this study we sought to investigate the activity and expression of
glutamate transport systems in an in vitro model of primary cultures of cochlear
glial cells obtained from newborn mice of the Balb/C lineage. For this, we
determined the sodium dependent and independent glutamate transport by
means of glutamate uptake and release assays whose extracellular
concentrations were quantified using High Performance Liquid Chromatography
coupled to a fluorescence detector. Finally, the cells were subjected to
immunofluorescence assay for XCG sodium-independent glutamate transporter
labeling. In our results, we demonstrate that cochlear glial cells have a
glutamate transport system mediated by the XCG- transporter. Such data
suggest a possible role of this transporter in the control of extracellular
concentrations of glutamate and regulation of the redox state, which may help
to preserve auditory function. |