Artigo

Methylmercury intoxication and histochemical demonstration of NADPH-diaphorase activity in the striate cortex of adult cats

The effects of methylmercury (MeHg) on histochemical demonstration of the NADPH-diaphorase (NADPH-d) activity in the striate cortex were studied in 4 adult cats. Two animals were used as control. The contaminated animals received 50 ml milk containing 0.42 µg MeHg and 100 g fish containing 0.03 µg M...

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Autor principal: OLIVEIRA, Ricardo Bezerra de
Outros Autores: LEAL, Walace Gomes, NASCIMENTO, José Luiz Martins do, DINIZ, Cristovam Wanderley Picanço
Grau: Artigo
Idioma: eng
Publicado em: 2012
Assuntos:
NADPH desidrogenase
Córtex visual
Neurotoxidade
Mercúrio
Acesso em linha: http://repositorio.ufpa.br/jspui/handle/2011/2581
id ir-2011-2581
recordtype dspace
spelling ir-2011-25812017-10-16T15:33:08Z Methylmercury intoxication and histochemical demonstration of NADPH-diaphorase activity in the striate cortex of adult cats OLIVEIRA, Ricardo Bezerra de LEAL, Walace Gomes NASCIMENTO, José Luiz Martins do DINIZ, Cristovam Wanderley Picanço NADPH desidrogenase Córtex visual Neurotoxidade Mercúrio The effects of methylmercury (MeHg) on histochemical demonstration of the NADPH-diaphorase (NADPH-d) activity in the striate cortex were studied in 4 adult cats. Two animals were used as control. The contaminated animals received 50 ml milk containing 0.42 µg MeHg and 100 g fish containing 0.03 µg MeHg daily for 2 months. The level of MeHg in area 17 of intoxicated animals was 3.2 µg/g wet weight brain tissue. Two cats were perfused 24 h after the last dose (group 1) and the other animals were perfused 6 months later (group 2). After microtomy, sections were processed for NADPHd histochemistry procedures using the malic enzyme method. Dendritic branch counts were performed from camera lucida drawings for control and intoxicated animals (N = 80). Average, standard deviation and Student t-test were calculated for each data group. The concentrations of mercury (Hg) in milk, fish and brain tissue were measured by acid digestion of samples, followed by reduction of total Hg in the digested sample to metallic Hg using stannous chloride followed by atomic fluorescence analysis. Only group 2 revealed a reduction of the neuropil enzyme activity and morphometric analysis showed a reduction in dendritic field area and in the number of distal dendrite branches of the NADPHd neurons in the white matter (P<0.05). These results suggest that NADPHd neurons in the white matter are more vulnerable to the long-term effects of MeHg than NADPHd neurons in the gray matter. 2012-03-29T20:21:58Z 2012-03-29T20:21:58Z 1998-09 Artigo de Periódico OLIVEIRA, R.B. et al. Methylmercury intoxication and histochemical demonstration of NADPH-diaphorase activity in the striate cortex of adult cats. Brazilian Journal of Medical and Biological Research, Ribeirão Preto, v. 31, n. 9, p. 1157-1161, set. 1998. Disponível em: <http://www.scielo.br/pdf/bjmbr/v31n9/3215c.pdf>. Acesso em: 28 mar. 2012. <http://dx.doi.org/10.1590/S0100-879X1998000900009>. 1414-431X http://repositorio.ufpa.br/jspui/handle/2011/2581 eng Acesso Aberto application/pdf
institution Repositório Institucional - Universidade Federal do Pará
collection RI-UFPA
language eng
topic NADPH desidrogenase
Córtex visual
Neurotoxidade
Mercúrio
spellingShingle NADPH desidrogenase
Córtex visual
Neurotoxidade
Mercúrio
OLIVEIRA, Ricardo Bezerra de
Methylmercury intoxication and histochemical demonstration of NADPH-diaphorase activity in the striate cortex of adult cats
topic_facet NADPH desidrogenase
Córtex visual
Neurotoxidade
Mercúrio
description The effects of methylmercury (MeHg) on histochemical demonstration of the NADPH-diaphorase (NADPH-d) activity in the striate cortex were studied in 4 adult cats. Two animals were used as control. The contaminated animals received 50 ml milk containing 0.42 µg MeHg and 100 g fish containing 0.03 µg MeHg daily for 2 months. The level of MeHg in area 17 of intoxicated animals was 3.2 µg/g wet weight brain tissue. Two cats were perfused 24 h after the last dose (group 1) and the other animals were perfused 6 months later (group 2). After microtomy, sections were processed for NADPHd histochemistry procedures using the malic enzyme method. Dendritic branch counts were performed from camera lucida drawings for control and intoxicated animals (N = 80). Average, standard deviation and Student t-test were calculated for each data group. The concentrations of mercury (Hg) in milk, fish and brain tissue were measured by acid digestion of samples, followed by reduction of total Hg in the digested sample to metallic Hg using stannous chloride followed by atomic fluorescence analysis. Only group 2 revealed a reduction of the neuropil enzyme activity and morphometric analysis showed a reduction in dendritic field area and in the number of distal dendrite branches of the NADPHd neurons in the white matter (P<0.05). These results suggest that NADPHd neurons in the white matter are more vulnerable to the long-term effects of MeHg than NADPHd neurons in the gray matter.
format Artigo
author OLIVEIRA, Ricardo Bezerra de
author2 LEAL, Walace Gomes
NASCIMENTO, José Luiz Martins do
DINIZ, Cristovam Wanderley Picanço
author2Str LEAL, Walace Gomes
NASCIMENTO, José Luiz Martins do
DINIZ, Cristovam Wanderley Picanço
title Methylmercury intoxication and histochemical demonstration of NADPH-diaphorase activity in the striate cortex of adult cats
title_short Methylmercury intoxication and histochemical demonstration of NADPH-diaphorase activity in the striate cortex of adult cats
title_full Methylmercury intoxication and histochemical demonstration of NADPH-diaphorase activity in the striate cortex of adult cats
title_fullStr Methylmercury intoxication and histochemical demonstration of NADPH-diaphorase activity in the striate cortex of adult cats
title_full_unstemmed Methylmercury intoxication and histochemical demonstration of NADPH-diaphorase activity in the striate cortex of adult cats
title_sort methylmercury intoxication and histochemical demonstration of nadph-diaphorase activity in the striate cortex of adult cats
publishDate 2012
url http://repositorio.ufpa.br/jspui/handle/2011/2581
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score 11.755432

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