Eleutherine plicata Herb. is an Iridaceae, popularly known as marupazinho,
widely used by people to treat diarrhea. With the bulb of the plant is about a cup of
tea, which is used to treat infestations caused by amoeba. The plant material used
in this study was collected in Belém do Pará and their identification by comparison
of botany was deposited in exsiccates Paraense Emilio Goeldi Museum under nº
10543. The ethanol extract was prepared by percolation from the bulb previously
dried and crushed. After drying the ethanol extract was suspended in a solution
hydroalcoholic (1:1) and subjected to partition with solvents of increasing polarity.
With the ethanol extract and the fractions were performed eighteen tests to detect
classes of secondary metabolites. The ethanol extract and the fractions hexanic
and chloroform, showed a positive result for naphthoquinones, anthraquinones
and steroids and triperpenoids. The analysis by thin-layer chromatography
fractions of ethanol extract and hexane and chloroform, showed areas susceptible
to methanolic solution of KOH 10%, indicating the presence of quinones in these
samples. The evaluation of the antimicrobial activity of ethanol extract and
fractions hexanic and chloroform with strains of C. albicans, S. aureus, E. coli and
P. aeruginosa, showed that the chloroform fraction is the most active, presenting
the largest halos of inhibition of microbial growth, possibly containing a higher
concentration of active constituents. The chemical constituents isoeleutherol and
isoeleutherine were isolated from chloroform fraction, and were characterized
chemically using RMN 1H e 13C, compared with the literature. The ethanol extract,
isoeleutherol and isoeleutherine were submitted to evaluation of their antioxidant
activies, and showed weak activity when compared with BHT.
|
