Diversity of Chromobacterium violaceum isolates from aquatic environments of state of Pará, Brazilian Amazon
The present study intended to characterize the phenotypic and genetic diversity of Brazilian isolates of Chromobacterium violaceum from aquatic environments within the Amazon region. Nineteen isolates showed morphological properties of C. violaceum and the majority grew at 44°C. Low temperatures, in...
| Autor principal: | DALL'AGNOL, Leonardo Teixeira |
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| Outros Autores: | MARTINS, Rosimar Neris, VALLINOTO, Antonio Carlos Rosário, RIBEIRO, Karla Tereza Silva |
| Grau: | Artigo |
| Idioma: | eng |
| Publicado em: |
2013
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| Acesso em linha: |
http://repositorio.ufpa.br/jspui/handle/2011/3611 |
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ir-2011-3611 |
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ir-2011-36112019-05-23T11:11:45Z Diversity of Chromobacterium violaceum isolates from aquatic environments of state of Pará, Brazilian Amazon DALL'AGNOL, Leonardo Teixeira MARTINS, Rosimar Neris VALLINOTO, Antonio Carlos Rosário RIBEIRO, Karla Tereza Silva Ambiente aquático Chromobacterium violaceum Variação genética Amazônia brasileira The present study intended to characterize the phenotypic and genetic diversity of Brazilian isolates of Chromobacterium violaceum from aquatic environments within the Amazon region. Nineteen isolates showed morphological properties of C. violaceum and the majority grew at 44°C. Low temperatures, in contrast, showed to be inhibitory to their growth, as eleven isolates did not grow at 10ºC and nine did not produce pigmentation, clearly indicating an inhibition of their metabolism. The largest variation among isolates was observed in the citrate test (Simmons), in which 12 isolates were positive, and in the oxidation/fermentation of sucrose, with six positives isolates. Chloramphenicol, gentamicin and sulfonamides efficiently inhibited bacterial growth. Amplified products of the recA gene were digested with HindII or PstI, which produced three or four restriction fragments patterns, respectively. The combined analysis arranged the isolates into six genospecies. The higher diversity observed in Belém (genotypes C, D, E and F) may be a consequence of intense human occupation, pollution of the aquatic environment or due to the higher diversity of the environments sampled in that region. In conclusion, a high level of genetic and phenotypic diversity was observed, and four new genospecies were described. 2013-04-02T12:58:21Z 2013-04-02T12:58:21Z 2008-11 Artigo de Periódico DALL'AGNOL, L. T., et al. Diversity of Chromobacterium violaceum isolates from aquatic environments of state of Pará, Brazilian Amazon. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 103, n. 7, p. 678-682, nov. 2008. Disponível em: <http://www.scielo.br/pdf/mioc/v103n7/09.pdf>. Acesso em: 01 abr. 2013. <http://dx.doi.org/10.1590/S0074-02762008000700009>. 0074-0276 http://repositorio.ufpa.br/jspui/handle/2011/3611 eng Acesso Aberto application/pdf |
| institution |
Repositório Institucional - Universidade Federal do Pará |
| collection |
RI-UFPA |
| language |
eng |
| topic |
Ambiente aquático Chromobacterium violaceum Variação genética Amazônia brasileira |
| spellingShingle |
Ambiente aquático Chromobacterium violaceum Variação genética Amazônia brasileira DALL'AGNOL, Leonardo Teixeira Diversity of Chromobacterium violaceum isolates from aquatic environments of state of Pará, Brazilian Amazon |
| topic_facet |
Ambiente aquático Chromobacterium violaceum Variação genética Amazônia brasileira |
| description |
The present study intended to characterize the phenotypic and genetic diversity of Brazilian isolates of Chromobacterium violaceum from aquatic environments within the Amazon region. Nineteen isolates showed morphological properties of C. violaceum and the majority grew at 44°C. Low temperatures, in contrast, showed to be inhibitory to their growth, as eleven isolates did not grow at 10ºC and nine did not produce pigmentation, clearly indicating an inhibition of their metabolism. The largest variation among isolates was observed in the citrate test (Simmons), in which 12 isolates were positive, and in the oxidation/fermentation of sucrose, with six positives isolates. Chloramphenicol, gentamicin and sulfonamides efficiently inhibited bacterial growth. Amplified products of the recA gene were digested with HindII or PstI, which produced three or four restriction fragments patterns, respectively. The combined analysis arranged the isolates into six genospecies. The higher diversity observed in Belém (genotypes C, D, E and F) may be a consequence of intense human occupation, pollution of the aquatic environment or due to the higher diversity of the environments sampled in that region. In conclusion, a high level of genetic and phenotypic diversity was observed, and four new genospecies were described. |
| format |
Artigo |
| author |
DALL'AGNOL, Leonardo Teixeira |
| author2 |
MARTINS, Rosimar Neris VALLINOTO, Antonio Carlos Rosário RIBEIRO, Karla Tereza Silva |
| author2Str |
MARTINS, Rosimar Neris VALLINOTO, Antonio Carlos Rosário RIBEIRO, Karla Tereza Silva |
| title |
Diversity of Chromobacterium violaceum isolates from aquatic environments of state of Pará, Brazilian Amazon |
| title_short |
Diversity of Chromobacterium violaceum isolates from aquatic environments of state of Pará, Brazilian Amazon |
| title_full |
Diversity of Chromobacterium violaceum isolates from aquatic environments of state of Pará, Brazilian Amazon |
| title_fullStr |
Diversity of Chromobacterium violaceum isolates from aquatic environments of state of Pará, Brazilian Amazon |
| title_full_unstemmed |
Diversity of Chromobacterium violaceum isolates from aquatic environments of state of Pará, Brazilian Amazon |
| title_sort |
diversity of chromobacterium violaceum isolates from aquatic environments of state of pará, brazilian amazon |
| publishDate |
2013 |
| url |
http://repositorio.ufpa.br/jspui/handle/2011/3611 |
| _version_ |
1832604338193694720 |
| score |
11.755432 |