Detection of HTLV-IIa in blood donors in an urban area of the Amazon Region of Brazil (Belém, PA)
The human lymphotropic viruses type I (HTLV-I) and type II (HTLV-II) are members of a group of mammalian retroviruses with similar biological properties, and blood transfusion is an important route of transmission. HTLV-I is endemic in a number of different geographical areas and is associated with...
| Autor principal: | ISHAK, Ricardo |
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| Outros Autores: | ISHAK, Marluísa de Oliveira Guimarães, SANTOS, Domingos Ezenildo Matos dos, VALLINOTO, Antonio Carlos Rosário, SARAIVA, João Carlos Pina, CRESCENTE, Jose Angelo Barletta, HALL, William W., AZEVEDO, Vânia Nakauth |
| Grau: | Artigo |
| Idioma: | eng |
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2013
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http://repositorio.ufpa.br/jspui/handle/2011/3612 |
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ir-2011-3612 |
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ir-2011-36122019-05-23T11:11:45Z Detection of HTLV-IIa in blood donors in an urban area of the Amazon Region of Brazil (Belém, PA) Detecção de HTLV-IIa em doadores de sangue em uma área urbana da Amazônia Brasileira (Belém, PA) ISHAK, Ricardo ISHAK, Marluísa de Oliveira Guimarães SANTOS, Domingos Ezenildo Matos dos VALLINOTO, Antonio Carlos Rosário SARAIVA, João Carlos Pina CRESCENTE, Jose Angelo Barletta HALL, William W. AZEVEDO, Vânia Nakauth Vírus 2 linfotrópico T humano Retroviridae Doadores de sangue Sangue Belém - PA Pará - Estado Amazônia brasileira The human lymphotropic viruses type I (HTLV-I) and type II (HTLV-II) are members of a group of mammalian retroviruses with similar biological properties, and blood transfusion is an important route of transmission. HTLV-I is endemic in a number of different geographical areas and is associated with several clinical disorders. HTLV-II is endemic in several Indian groups of the Americas and intravenous drug abusers in North and South America, Europe and Southeast Asia. During the year of 1995, all blood donors tested positive to HTLV-I/II in the State Blood Bank (HEMOPA), were directed to a physician and to the Virus Laboratory at the Universidade Federal do Pará for counselling and laboratory diagnosis confirmation. Thirty-five sera were tested by an enzyme immune assay, and a Western blot that discriminates HTLV-I and HTLV-II infection. Two HTLV-II positive samples were submitted to PCR analysis of pX and env genomic region, and confirmed to be of subtype IIa. This is the first detection in Belém of the presence of HTLV-IIa infection among blood donors. This result emphasizes that HTLV-II is also present in urban areas of the Amazon region of Brazil and highlights the need to include screening tests that are capable to detect antibodies for both types of HTLV. Os vírus linfotrópicos de células T humanas tipo I (HTLV-I) e tipo II (HTLV-II) são membros de um grupo de retrovírus de mamíferos com propriedades biológicas similares que apresentam como uma das principais rotas de transmissão a transfusão sangüínea. O HTLV-I é endêmico em diferentes áreas geográficas e está associado a vários distúrbios clínicos. O HTLV-II é endêmico em vários grupos indígenas das Américas e em usuários de drogas intravenosas na América do Norte e do Sul, Europa e Sudeste da Ásia. Durante o ano de 1995, todos os doadores de sangue positivos para HTLV-I/II no Banco de Sangue do Estado (HEMOPA), foram direcionados a um médico e ao Laboratório de Virologia na Universidade Federal do Pará, para consulta, aconselhamento e confirmação do diagnóstico laboratorial. Trinta e cinco soros foram testados por um ensaio imunoenzimático e confirmados por um Western blot que discrimina as infecções por HTLV-I e HTLV-II. Amostras soropositivas para HTLV-II foram submetidas à reação em cadeia da polimerase (PCR) para as regiões genômicas env e pX e confirmaram ser do subtipo IIa. Esta é a primeira detecção, em Belém, da presença da infecção pelo HTLV-IIa em doadores de sangue. Estes resultados enfatizam que o HTLV-II está presente em áreas urbanas da região Amazônica e a necessidade de incluir testes de triagem capazes de detectar anticorpos para ambos os tipos de HTLV. 2013-04-02T13:17:40Z 2013-04-02T13:17:40Z 1998-04 Artigo de Periódico ISHAK, R., et al. Detection of HTLV-IIa in blood donors in an urban area of the Amazon Region of Brazil (Belém, PA). Revista da Sociedade Brasileira de Medicina Tropical. Uberaba, v. 31, n. 2, p. 193-197, mar./abr. 1998. Disponível em: <http://www.scielo.br/pdf/rsbmt/v31n2/0599.pdf>. Acesso em: 01 abr. 2013. <http://dx.doi.org/10.1590/S0037-86821998000200005>. 1678-9849 0037-8682 http://repositorio.ufpa.br/jspui/handle/2011/3612 eng Acesso Aberto application/pdf |
| institution |
Repositório Institucional - Universidade Federal do Pará |
| collection |
RI-UFPA |
| language |
eng |
| topic |
Vírus 2 linfotrópico T humano Retroviridae Doadores de sangue Sangue Belém - PA Pará - Estado Amazônia brasileira |
| spellingShingle |
Vírus 2 linfotrópico T humano Retroviridae Doadores de sangue Sangue Belém - PA Pará - Estado Amazônia brasileira ISHAK, Ricardo Detection of HTLV-IIa in blood donors in an urban area of the Amazon Region of Brazil (Belém, PA) |
| topic_facet |
Vírus 2 linfotrópico T humano Retroviridae Doadores de sangue Sangue Belém - PA Pará - Estado Amazônia brasileira |
| description |
The human lymphotropic viruses type I (HTLV-I) and type II (HTLV-II) are members of a group of mammalian retroviruses with similar biological properties, and blood transfusion is an important route of transmission. HTLV-I is endemic in a number of different geographical areas and is associated with several clinical disorders. HTLV-II is endemic in several Indian groups of the Americas and intravenous drug abusers in North and South America, Europe and Southeast Asia. During the year of 1995, all blood donors tested positive to HTLV-I/II in the State Blood Bank (HEMOPA), were directed to a physician and to the Virus Laboratory at the Universidade Federal do Pará for counselling and laboratory diagnosis confirmation. Thirty-five sera were tested by an enzyme immune assay, and a Western blot that discriminates HTLV-I and HTLV-II infection. Two HTLV-II positive samples were submitted to PCR analysis of pX and env genomic region, and confirmed to be of subtype IIa. This is the first detection in Belém of the presence of HTLV-IIa infection among blood donors. This result emphasizes that HTLV-II is also present in urban areas of the Amazon region of Brazil and highlights the need to include screening tests that are capable to detect antibodies for both types of HTLV. |
| format |
Artigo |
| author |
ISHAK, Ricardo |
| author2 |
ISHAK, Marluísa de Oliveira Guimarães SANTOS, Domingos Ezenildo Matos dos VALLINOTO, Antonio Carlos Rosário SARAIVA, João Carlos Pina CRESCENTE, Jose Angelo Barletta HALL, William W. AZEVEDO, Vânia Nakauth |
| author2Str |
ISHAK, Marluísa de Oliveira Guimarães SANTOS, Domingos Ezenildo Matos dos VALLINOTO, Antonio Carlos Rosário SARAIVA, João Carlos Pina CRESCENTE, Jose Angelo Barletta HALL, William W. AZEVEDO, Vânia Nakauth |
| title |
Detection of HTLV-IIa in blood donors in an urban area of the Amazon Region of Brazil (Belém, PA) |
| title_short |
Detection of HTLV-IIa in blood donors in an urban area of the Amazon Region of Brazil (Belém, PA) |
| title_full |
Detection of HTLV-IIa in blood donors in an urban area of the Amazon Region of Brazil (Belém, PA) |
| title_fullStr |
Detection of HTLV-IIa in blood donors in an urban area of the Amazon Region of Brazil (Belém, PA) |
| title_full_unstemmed |
Detection of HTLV-IIa in blood donors in an urban area of the Amazon Region of Brazil (Belém, PA) |
| title_sort |
detection of htlv-iia in blood donors in an urban area of the amazon region of brazil (belém, pa) |
| publishDate |
2013 |
| url |
http://repositorio.ufpa.br/jspui/handle/2011/3612 |
| _version_ |
1832602953743073280 |
| score |
11.755432 |