Characterization of polymorphisms in the mannose-binding lectin gene promoter among human immunodeficiency virus 1 infected subjects

VALLINOTO, Antonio Carlos Rosário; MUTO, Nilton Akio; ALVES, Anna Elizabeth Martins; MACHADO, Luiz Fernando Almeida; AZEVEDO, Vânia Nakauth; SOUZA, Lia Lobato Batista de; ISHAK, Marluísa de Oliveira Guimarães; ISHAK, Ricardo

Artigo

Characterization of polymorphisms in the mannose-binding lectin gene promoter among human immunodeficiency virus 1 infected subjects

The present study investigated the prevalence of mutations in the -550 (H/L) and -221 (X/Y) mannose-binding lectin (MBL) gene promoter regions and their impact on infection by human immunodeficiency virus 1 (HIV-1) in a population of 128 HIV-1 seropositive and 97 seronegative patients. The allele id...

ver descrição completa

Autor principal:	VALLINOTO, Antonio Carlos Rosário
Outros Autores:	MUTO, Nilton Akio, ALVES, Anna Elizabeth Martins, MACHADO, Luiz Fernando Almeida, AZEVEDO, Vânia Nakauth, SOUZA, Lia Lobato Batista de, ISHAK, Marluísa de Oliveira Guimarães, ISHAK, Ricardo
Grau:	Artigo
Idioma:	eng
Publicado em:	2013
Assuntos:	Polimorfismo genético MBL HIV-1 Prevalência Belém - PA Pará - Estado Amazônia brasileira
Acesso em linha:	http://repositorio.ufpa.br/jspui/handle/2011/3620

id	ir-2011-3620
recordtype	dspace
spelling	ir-2011-36202019-05-23T11:11:45Z Characterization of polymorphisms in the mannose-binding lectin gene promoter among human immunodeficiency virus 1 infected subjects VALLINOTO, Antonio Carlos Rosário MUTO, Nilton Akio ALVES, Anna Elizabeth Martins MACHADO, Luiz Fernando Almeida AZEVEDO, Vânia Nakauth SOUZA, Lia Lobato Batista de ISHAK, Marluísa de Oliveira Guimarães ISHAK, Ricardo Polimorfismo genético MBL HIV-1 Prevalência Belém - PA Pará - Estado Amazônia brasileira The present study investigated the prevalence of mutations in the -550 (H/L) and -221 (X/Y) mannose-binding lectin (MBL) gene promoter regions and their impact on infection by human immunodeficiency virus 1 (HIV-1) in a population of 128 HIV-1 seropositive and 97 seronegative patients. The allele identification was performed through the sequence-specific primer polymerase chain reaction method, using primer sequences specific to each polymorphism. The evolution of the infection was evaluated through CD4+ T-lymphocyte counts and plasma viral load. The allele and haplotype frequencies among HIV-1-infected patients and seronegative healthy control patients did not show significant differences. CD4+ T-lymphocyte counts showed lower levels among seropositive patients carrying haplotypes LY, LX and HX, as compared to those carrying the HY haplotype. Mean plasma viral load was higher among seropositive patients with haplotypes LY, LX and HX than among those carrying the HY haplotype. When promoter and exon 1 mutations were matched, it was possible to identify a significantly higher viral load among HIV-1 infected individuals carrying haplotypes correlated to low serum levels of MBL. The current study shows that haplotypes related to medium and low MBL serum levels might directly influence the evolution of viral progression in patients. Therefore, it is suggested that the identification of haplotypes within the promoter region of the MBL gene among HIV-1 infected persons should be further evaluated as a prognostic tool for AIDS progression. 2013-04-03T12:53:27Z 2013-04-03T12:53:27Z 2008-11 Artigo de Periódico VALLINOTO, A. C. R., et al. Characterization of polymorphisms in the mannose-binding lectin gene promoter among human immunodeficiency virus 1 infected subjects. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 103, n. 7, p. 645-649, nov. 2008. Disponível em: <http://www.scielo.br/pdf/mioc/v103n7/03.pdf>. Acesso em: 02 abr. 2013. <http://dx.doi.org/10.1590/S0074-02762008000700003>. 1678-8060 0074-0276 http://repositorio.ufpa.br/jspui/handle/2011/3620 eng Acesso Aberto application/pdf
institution	Repositório Institucional - Universidade Federal do Pará
collection	RI-UFPA
language	eng
topic	Polimorfismo genético MBL HIV-1 Prevalência Belém - PA Pará - Estado Amazônia brasileira
spellingShingle	Polimorfismo genético MBL HIV-1 Prevalência Belém - PA Pará - Estado Amazônia brasileira VALLINOTO, Antonio Carlos Rosário Characterization of polymorphisms in the mannose-binding lectin gene promoter among human immunodeficiency virus 1 infected subjects
topic_facet	Polimorfismo genético MBL HIV-1 Prevalência Belém - PA Pará - Estado Amazônia brasileira
description	The present study investigated the prevalence of mutations in the -550 (H/L) and -221 (X/Y) mannose-binding lectin (MBL) gene promoter regions and their impact on infection by human immunodeficiency virus 1 (HIV-1) in a population of 128 HIV-1 seropositive and 97 seronegative patients. The allele identification was performed through the sequence-specific primer polymerase chain reaction method, using primer sequences specific to each polymorphism. The evolution of the infection was evaluated through CD4+ T-lymphocyte counts and plasma viral load. The allele and haplotype frequencies among HIV-1-infected patients and seronegative healthy control patients did not show significant differences. CD4+ T-lymphocyte counts showed lower levels among seropositive patients carrying haplotypes LY, LX and HX, as compared to those carrying the HY haplotype. Mean plasma viral load was higher among seropositive patients with haplotypes LY, LX and HX than among those carrying the HY haplotype. When promoter and exon 1 mutations were matched, it was possible to identify a significantly higher viral load among HIV-1 infected individuals carrying haplotypes correlated to low serum levels of MBL. The current study shows that haplotypes related to medium and low MBL serum levels might directly influence the evolution of viral progression in patients. Therefore, it is suggested that the identification of haplotypes within the promoter region of the MBL gene among HIV-1 infected persons should be further evaluated as a prognostic tool for AIDS progression.
format	Artigo
author	VALLINOTO, Antonio Carlos Rosário
author2	MUTO, Nilton Akio ALVES, Anna Elizabeth Martins MACHADO, Luiz Fernando Almeida AZEVEDO, Vânia Nakauth SOUZA, Lia Lobato Batista de ISHAK, Marluísa de Oliveira Guimarães ISHAK, Ricardo
author2Str	MUTO, Nilton Akio ALVES, Anna Elizabeth Martins MACHADO, Luiz Fernando Almeida AZEVEDO, Vânia Nakauth SOUZA, Lia Lobato Batista de ISHAK, Marluísa de Oliveira Guimarães ISHAK, Ricardo
title	Characterization of polymorphisms in the mannose-binding lectin gene promoter among human immunodeficiency virus 1 infected subjects
title_short	Characterization of polymorphisms in the mannose-binding lectin gene promoter among human immunodeficiency virus 1 infected subjects
title_full	Characterization of polymorphisms in the mannose-binding lectin gene promoter among human immunodeficiency virus 1 infected subjects
title_fullStr	Characterization of polymorphisms in the mannose-binding lectin gene promoter among human immunodeficiency virus 1 infected subjects
title_full_unstemmed	Characterization of polymorphisms in the mannose-binding lectin gene promoter among human immunodeficiency virus 1 infected subjects
title_sort	characterization of polymorphisms in the mannose-binding lectin gene promoter among human immunodeficiency virus 1 infected subjects
publishDate	2013
url	http://repositorio.ufpa.br/jspui/handle/2011/3620
_version_	1832602813097574400
score	11.755432

Characterization of polymorphisms in the mannose-binding lectin gene promoter among human immunodeficiency virus 1 infected subjects

Registros relacionados