Molecular characterization of human T-cell lymphotropic virus coinfecting human immunodeficiency virus 1 infected patients in the Amazon region of Brazil
The present work evaluated the epidemiology of human immunodeficiency virus 1/human T-cell lymphotropic virus (HIV-1/HTLV) coinfection in patients living in Belém (state of Pará) and Macapá (state of Amapá), two cities located in the Amazon region of Brazil. A total of 169 blood samples were collect...
| Autor principal: | LAURENTINO, Rogério Valois |
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| Outros Autores: | LOPES, Ivina Giselle Lima, AZEVEDO, Vânia Nakauth, MACHADO, Luiz Fernando Almeida, MOREIRA, Márcio Ronaldo Chagas, SOUZA, Lia Lobato Batista de, ISHAK, Marluísa de Oliveira Guimarães, ISHAK, Ricardo, VALLINOTO, Antonio Carlos Rosário |
| Grau: | Artigo |
| Idioma: | eng |
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2013
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http://repositorio.ufpa.br/jspui/handle/2011/3643 |
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ir-2011-3643 |
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ir-2011-36432019-05-23T11:11:45Z Molecular characterization of human T-cell lymphotropic virus coinfecting human immunodeficiency virus 1 infected patients in the Amazon region of Brazil LAURENTINO, Rogério Valois LOPES, Ivina Giselle Lima AZEVEDO, Vânia Nakauth MACHADO, Luiz Fernando Almeida MOREIRA, Márcio Ronaldo Chagas SOUZA, Lia Lobato Batista de ISHAK, Marluísa de Oliveira Guimarães ISHAK, Ricardo VALLINOTO, Antonio Carlos Rosário HIV-1 Vírus linfotrópico de células T humanas tipo 1 Diagnóstico laboratorial Epidemiologia molecular Vírus 2 linfotrópico T humano Belém - PA Macapá - AP Pará - Estado Amapá - Estado Amazônia brasileira The present work evaluated the epidemiology of human immunodeficiency virus 1/human T-cell lymphotropic virus (HIV-1/HTLV) coinfection in patients living in Belém (state of Pará) and Macapá (state of Amapá), two cities located in the Amazon region of Brazil. A total of 169 blood samples were collected. The sera were tested by enzyme-linked immunosorbent assay to determine the presence of antibodies anti-HTLV-1/2. Confirmation of infection and discrimination of HTLV types and subtypes was performed using a nested polymerase chain reaction targeting the pX and 5' LTR regions, followed by restriction fragment length polymorphism and sequencing analysis. The presence of anti-HTLV1/2 was detected in six patients from Belém. The amplification of the pX region followed by RFLP analysis, demonstrated the presence of HTLV-1 and HTLV-2 infections among two and four patients, respectively. Sequencing HTLV-1 5' LTR indicated that the virus is a member of the Cosmopolitan Group, Transcontinental subgroup. HTLV-2 strains isolated revealed a molecular profile of subtype HTLV-2c. These results are a reflex of the epidemiological features of HIV-1/HTLV-1/2 coinfection in the North region of Brazil, which is distinct from other Brazilian regions, as reported by previous studies. 2013-04-06T22:39:44Z 2013-04-06T22:39:44Z 2005-07 Artigo de Periódico LAURENTINO, R. V., et al. Molecular characterization of human T-cell lymphotropic virus coinfecting human immunodeficiency virus 1 infected patients in the Amazon region of Brazil. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 100, n. 4, p. 371-376, jul. 2005. Disponível em: <http://www.scielo.br/pdf/mioc/v100n4/v100n4a06.pdf>. Acesso em: 03 abr. 2013. <http://dx.doi.org/10.1590/S0074-02762005000400006>. 1678-8060 0074-0276 http://repositorio.ufpa.br/jspui/handle/2011/3643 eng Acesso Aberto application/pdf |
| institution |
Repositório Institucional - Universidade Federal do Pará |
| collection |
RI-UFPA |
| language |
eng |
| topic |
HIV-1 Vírus linfotrópico de células T humanas tipo 1 Diagnóstico laboratorial Epidemiologia molecular Vírus 2 linfotrópico T humano Belém - PA Macapá - AP Pará - Estado Amapá - Estado Amazônia brasileira |
| spellingShingle |
HIV-1 Vírus linfotrópico de células T humanas tipo 1 Diagnóstico laboratorial Epidemiologia molecular Vírus 2 linfotrópico T humano Belém - PA Macapá - AP Pará - Estado Amapá - Estado Amazônia brasileira LAURENTINO, Rogério Valois Molecular characterization of human T-cell lymphotropic virus coinfecting human immunodeficiency virus 1 infected patients in the Amazon region of Brazil |
| topic_facet |
HIV-1 Vírus linfotrópico de células T humanas tipo 1 Diagnóstico laboratorial Epidemiologia molecular Vírus 2 linfotrópico T humano Belém - PA Macapá - AP Pará - Estado Amapá - Estado Amazônia brasileira |
| description |
The present work evaluated the epidemiology of human immunodeficiency virus 1/human T-cell lymphotropic virus (HIV-1/HTLV) coinfection in patients living in Belém (state of Pará) and Macapá (state of Amapá), two cities located in the Amazon region of Brazil. A total of 169 blood samples were collected. The sera were tested by enzyme-linked immunosorbent assay to determine the presence of antibodies anti-HTLV-1/2. Confirmation of infection and discrimination of HTLV types and subtypes was performed using a nested polymerase chain reaction targeting the pX and 5' LTR regions, followed by restriction fragment length polymorphism and sequencing analysis. The presence of anti-HTLV1/2 was detected in six patients from Belém. The amplification of the pX region followed by RFLP analysis, demonstrated the presence of HTLV-1 and HTLV-2 infections among two and four patients, respectively. Sequencing HTLV-1 5' LTR indicated that the virus is a member of the Cosmopolitan Group, Transcontinental subgroup. HTLV-2 strains isolated revealed a molecular profile of subtype HTLV-2c. These results are a reflex of the epidemiological features of HIV-1/HTLV-1/2 coinfection in the North region of Brazil, which is distinct from other Brazilian regions, as reported by previous studies. |
| format |
Artigo |
| author |
LAURENTINO, Rogério Valois |
| author2 |
LOPES, Ivina Giselle Lima AZEVEDO, Vânia Nakauth MACHADO, Luiz Fernando Almeida MOREIRA, Márcio Ronaldo Chagas SOUZA, Lia Lobato Batista de ISHAK, Marluísa de Oliveira Guimarães ISHAK, Ricardo VALLINOTO, Antonio Carlos Rosário |
| author2Str |
LOPES, Ivina Giselle Lima AZEVEDO, Vânia Nakauth MACHADO, Luiz Fernando Almeida MOREIRA, Márcio Ronaldo Chagas SOUZA, Lia Lobato Batista de ISHAK, Marluísa de Oliveira Guimarães ISHAK, Ricardo VALLINOTO, Antonio Carlos Rosário |
| title |
Molecular characterization of human T-cell lymphotropic virus coinfecting human immunodeficiency virus 1 infected patients in the Amazon region of Brazil |
| title_short |
Molecular characterization of human T-cell lymphotropic virus coinfecting human immunodeficiency virus 1 infected patients in the Amazon region of Brazil |
| title_full |
Molecular characterization of human T-cell lymphotropic virus coinfecting human immunodeficiency virus 1 infected patients in the Amazon region of Brazil |
| title_fullStr |
Molecular characterization of human T-cell lymphotropic virus coinfecting human immunodeficiency virus 1 infected patients in the Amazon region of Brazil |
| title_full_unstemmed |
Molecular characterization of human T-cell lymphotropic virus coinfecting human immunodeficiency virus 1 infected patients in the Amazon region of Brazil |
| title_sort |
molecular characterization of human t-cell lymphotropic virus coinfecting human immunodeficiency virus 1 infected patients in the amazon region of brazil |
| publishDate |
2013 |
| url |
http://repositorio.ufpa.br/jspui/handle/2011/3643 |
| _version_ |
1832605119018958848 |
| score |
11.755432 |