Susceptibility of Cebus apella monkey (Primates: Cebidae) to experimental Leishmania (L.) infantum chagasi-infection

CARNEIRO, Liliane Almeida; SILVEIRA, Fernando Tobias; CAMPOS, Marliane Batista; BRÍGIDO, Maria do Carmo de Oliveira; CORBETT, Carlos Eduardo Pereira; LAURENTI, Márcia Dalastra

Artigo

Susceptibility of Cebus apella monkey (Primates: Cebidae) to experimental Leishmania (L.) infantum chagasi-infection

In Amazonian Brazil, the Cebus apella monkey (Primates: Cebidae) has been associated with the enzootic cycle of Leishmania (V.) shawi, a dermotropic parasite causing American cutaneous leishmaniasis (ACL). It has also been successfully used as animal model for studying cutaneous leishmaniasis. In th...

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Autor principal:	CARNEIRO, Liliane Almeida
Outros Autores:	SILVEIRA, Fernando Tobias, CAMPOS, Marliane Batista, BRÍGIDO, Maria do Carmo de Oliveira, CORBETT, Carlos Eduardo Pereira, LAURENTI, Márcia Dalastra
Grau:	Artigo
Idioma:	eng
Publicado em:	2013
Assuntos:	Suscetibilidade a doenças Cebus apella Leishmania infantum Infecção experimental Amazônia brasileira
Acesso em linha:	http://repositorio.ufpa.br/jspui/handle/2011/3843

id	ir-2011-3843
recordtype	dspace
spelling	ir-2011-38432019-05-23T13:25:42Z Susceptibility of Cebus apella monkey (Primates: Cebidae) to experimental Leishmania (L.) infantum chagasi-infection Susceptibilidade do macaco Cebus apella (Primata: Cebidae) à infecção experimental por Leishmania (L.) infantum chagasi CARNEIRO, Liliane Almeida SILVEIRA, Fernando Tobias CAMPOS, Marliane Batista BRÍGIDO, Maria do Carmo de Oliveira CORBETT, Carlos Eduardo Pereira LAURENTI, Márcia Dalastra Suscetibilidade a doenças Cebus apella Leishmania infantum Infecção experimental Amazônia brasileira In Amazonian Brazil, the Cebus apella monkey (Primates: Cebidae) has been associated with the enzootic cycle of Leishmania (V.) shawi, a dermotropic parasite causing American cutaneous leishmaniasis (ACL). It has also been successfully used as animal model for studying cutaneous leishmaniasis. In this work, there has been investigated its susceptibility to experimental Leishmania (L.) infantum chagasi-infection, the etiologic agent of American visceral leishmaniasis (AVL). There were used ten C. apella specimens, eight adult and two young, four males and six females, all born and raised in captivity. Two experimental infection protocols were performed: i) six monkeys were inoculated, intra-dermal via (ID), into the base of the tail with 2 x 106 promastigotes forms from the stationary phase culture medium; ii) other four monkeys were inoculated with 3 x 107 amastigotes forms from the visceral infection of infected hamsters by two different via: a) two by intravenous via (IV) and, b) other two by intra-peritoneal via (IP). The parameters of infection evaluation included: a) clinical: physical exam of abdomen, weigh and body temperature; b) parasitological: needle aspiration of the bone-marrow for searching of amastigotes (Giemsa-stained smears) and promastigotes forms (culture medium); c) immunological: Indirect fluorescence antibody test (IFAT) and, Delayed-type hypersensitivity (DTH). In the six monkeys ID inoculated (promastigotes forms) all parameters of infection evaluation were negative during the 12 months period of follow-up. Among the four monkeys inoculated with amastigotes forms, two IV inoculated showed the parasite in the bone-marrow from the first toward to the sixth month p.i. and following that they cleared the infection, whereas the other two IP inoculated were totally negative. These four monkeys showed specific IgG-antibody response since the third month p.i. (IP: 1/80 and IV: 1/320 IgG) toward to the 12th month (IP: 1/160 and IV: 1/5120). The DTH-conversion occurred in only one IV inoculated monkey with a strong (30 mm) skin reaction. Considering these results, we do not encourage the use of C. apella monkey as animal model for studying the AVL. Na Amazônia Brasileira, o macaco Cebus apella (Primata: Cebidae) tem sido associado com o ciclo enzoótico da Leishmania (V.) shawi, um parasito dermotrópico causador da Leishmaniose Tegumentar Americana (LTA). Ele tem sido também empregado com sucesso como modelo experimental para estudo da leishmaniose tegumentar. Neste trabalho, foi investigada sua susceptibilidade à infecção experimental por Leishmania (L.) infantum chagasi, o agente etiológico da Leishmaniose Visceral Americana (LVA). Foram usados dez espécimes de C. apella oito adultos e dois jovens, quatro machos e seis fêmeas, todos nascidos e criados em cativeiro. Dois protocolos de infecção experimental foram feitos: i) seis macacos foram inoculados por via intradérmica (ID), na base da cauda com 2x106 formas promastigotas em fase estacionária de crescimento; ii) outros quatro macacos foram inoculados com 3x107 formas amastigotas de infecção visceral de hamsteres por duas vias diferentes: a) dois por via intravenosa (IV) e, b) outros dois pela via intraperitoneal (IP). A avaliação da infecção incluiu parâmetros: clínico: exame físico do abdômen, peso e temperatura corporal; b) parasitológico: aspirado de medula óssea por agulha para procura de amastigotas (esfregaço corado por Giemsa) e formas promastigotas (meio de cultura); c) imunológico: Reação de Imunofluorescência Indireta (RIFI) e, resposta de hipersensibilidade tardia (DTH). Nos seis macacos inoculados ID (formas promastigotas) todos os parâmetros de avaliação da infecção foram negativos durante o período de 12 meses. Entre os quatro macacos inoculados com formas amastigotas, dois inoculados IV mostraram parasitos na medula óssea do primeiro ao sexto mês p.i. e em seguida houve a resolução da infecção, no entanto os outros dois inoculados IP foram totalmente negativos. Esses quatro macacos apresentaram resposta específica de anticorpo IgG desde o terceiro mês p.i. (IP: 1/80 e IV: 1/320) até o décimo segundo mês (IP: 1/160 e IV: 1/5120). A conversão DTH ocorreu em apenas um macaco inoculado IV com uma forte reação na pele (30 mm). Considerando esses resultados, nós não recomendamos o uso do macaco C. apella como modelo animal para estudo da LVA. 2013-05-10T17:12:52Z 2013-05-10T17:12:52Z 2011-02 Artigo de Periódico CARNEIRO, Liliane Almeida, et al. Susceptibility of Cebus apella monkey (Primates: Cebidae) to experimental Leishmania (L.) infantum chagasi-infection. Revista do Instituto de Medicina Tropical de São Paulo, São Paulo, v. 53, n. 1, p. 45-50, jan./fev. 2011. Disponível em: <http://www.scielo.br/pdf/rimtsp/v53n1/v53n1a08.pdf>. Acesso em: 10 maio 2013. <http://dx.doi.org/10.1590/S0036-46652011000100008>. 0036-4665 1678-9946 http://repositorio.ufpa.br/jspui/handle/2011/3843 eng Acesso Aberto application/pdf
institution	Repositório Institucional - Universidade Federal do Pará
collection	RI-UFPA
language	eng
topic	Suscetibilidade a doenças Cebus apella Leishmania infantum Infecção experimental Amazônia brasileira
spellingShingle	Suscetibilidade a doenças Cebus apella Leishmania infantum Infecção experimental Amazônia brasileira CARNEIRO, Liliane Almeida Susceptibility of Cebus apella monkey (Primates: Cebidae) to experimental Leishmania (L.) infantum chagasi-infection
topic_facet	Suscetibilidade a doenças Cebus apella Leishmania infantum Infecção experimental Amazônia brasileira
description	In Amazonian Brazil, the Cebus apella monkey (Primates: Cebidae) has been associated with the enzootic cycle of Leishmania (V.) shawi, a dermotropic parasite causing American cutaneous leishmaniasis (ACL). It has also been successfully used as animal model for studying cutaneous leishmaniasis. In this work, there has been investigated its susceptibility to experimental Leishmania (L.) infantum chagasi-infection, the etiologic agent of American visceral leishmaniasis (AVL). There were used ten C. apella specimens, eight adult and two young, four males and six females, all born and raised in captivity. Two experimental infection protocols were performed: i) six monkeys were inoculated, intra-dermal via (ID), into the base of the tail with 2 x 106 promastigotes forms from the stationary phase culture medium; ii) other four monkeys were inoculated with 3 x 107 amastigotes forms from the visceral infection of infected hamsters by two different via: a) two by intravenous via (IV) and, b) other two by intra-peritoneal via (IP). The parameters of infection evaluation included: a) clinical: physical exam of abdomen, weigh and body temperature; b) parasitological: needle aspiration of the bone-marrow for searching of amastigotes (Giemsa-stained smears) and promastigotes forms (culture medium); c) immunological: Indirect fluorescence antibody test (IFAT) and, Delayed-type hypersensitivity (DTH). In the six monkeys ID inoculated (promastigotes forms) all parameters of infection evaluation were negative during the 12 months period of follow-up. Among the four monkeys inoculated with amastigotes forms, two IV inoculated showed the parasite in the bone-marrow from the first toward to the sixth month p.i. and following that they cleared the infection, whereas the other two IP inoculated were totally negative. These four monkeys showed specific IgG-antibody response since the third month p.i. (IP: 1/80 and IV: 1/320 IgG) toward to the 12th month (IP: 1/160 and IV: 1/5120). The DTH-conversion occurred in only one IV inoculated monkey with a strong (30 mm) skin reaction. Considering these results, we do not encourage the use of C. apella monkey as animal model for studying the AVL.
format	Artigo
author	CARNEIRO, Liliane Almeida
author2	SILVEIRA, Fernando Tobias CAMPOS, Marliane Batista BRÍGIDO, Maria do Carmo de Oliveira CORBETT, Carlos Eduardo Pereira LAURENTI, Márcia Dalastra
author2Str	SILVEIRA, Fernando Tobias CAMPOS, Marliane Batista BRÍGIDO, Maria do Carmo de Oliveira CORBETT, Carlos Eduardo Pereira LAURENTI, Márcia Dalastra
title	Susceptibility of Cebus apella monkey (Primates: Cebidae) to experimental Leishmania (L.) infantum chagasi-infection
title_short	Susceptibility of Cebus apella monkey (Primates: Cebidae) to experimental Leishmania (L.) infantum chagasi-infection
title_full	Susceptibility of Cebus apella monkey (Primates: Cebidae) to experimental Leishmania (L.) infantum chagasi-infection
title_fullStr	Susceptibility of Cebus apella monkey (Primates: Cebidae) to experimental Leishmania (L.) infantum chagasi-infection
title_full_unstemmed	Susceptibility of Cebus apella monkey (Primates: Cebidae) to experimental Leishmania (L.) infantum chagasi-infection
title_sort	susceptibility of cebus apella monkey (primates: cebidae) to experimental leishmania (l.) infantum chagasi-infection
publishDate	2013
url	http://repositorio.ufpa.br/jspui/handle/2011/3843
_version_	1787148149484158976
score	11.653393

Susceptibility of Cebus apella monkey (Primates: Cebidae) to experimental Leishmania (L.) infantum chagasi-infection

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