Diversity and three-dimensional structures of the alpha Mcr of the methanogenic Archaea from the anoxic region of Tucuruí Lake, in Eastern Brazilian Amazonia

SANTANA, Priscila di Paula Bessa; GHILARDI JUNIOR, Rubens; ALVES, Claudio Nahum; SILVA, Jeronimo Lameira; MCCULLOCH, John Anthony; SCHNEIDER, Maria Paula Cruz; SILVA, Artur Luiz da Costa da

Artigo

Diversity and three-dimensional structures of the alpha Mcr of the methanogenic Archaea from the anoxic region of Tucuruí Lake, in Eastern Brazilian Amazonia

Methanogenic archaeans are organisms of considerable ecological and biotechnological interest that produce methane through a restricted metabolic pathway, which culminates in the reaction catalyzed by the Methyl-coenzyme M reductase (Mcr) enzyme, and results in the release of methane. Using a metage...

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Autor principal:	SANTANA, Priscila di Paula Bessa
Outros Autores:	GHILARDI JUNIOR, Rubens, ALVES, Claudio Nahum, SILVA, Jeronimo Lameira, MCCULLOCH, John Anthony, SCHNEIDER, Maria Paula Cruz, SILVA, Artur Luiz da Costa da
Grau:	Artigo
Idioma:	eng
Publicado em:	2013
Assuntos:	Archaea Metil-coenzima M redutase Modelagem por homologia Lago de Tucuruí - PA Tucuruí - PA Pará - Estado Amazônia brasileira
Acesso em linha:	http://repositorio.ufpa.br/jspui/handle/2011/4094

id	ir-2011-4094
recordtype	dspace
spelling	ir-2011-40942019-05-23T11:11:45Z Diversity and three-dimensional structures of the alpha Mcr of the methanogenic Archaea from the anoxic region of Tucuruí Lake, in Eastern Brazilian Amazonia SANTANA, Priscila di Paula Bessa GHILARDI JUNIOR, Rubens ALVES, Claudio Nahum SILVA, Jeronimo Lameira MCCULLOCH, John Anthony SCHNEIDER, Maria Paula Cruz SILVA, Artur Luiz da Costa da Archaea Metil-coenzima M redutase Modelagem por homologia Lago de Tucuruí - PA Tucuruí - PA Pará - Estado Amazônia brasileira Methanogenic archaeans are organisms of considerable ecological and biotechnological interest that produce methane through a restricted metabolic pathway, which culminates in the reaction catalyzed by the Methyl-coenzyme M reductase (Mcr) enzyme, and results in the release of methane. Using a metagenomic approach, the gene of the a subunit of mcr (mcrα) was isolated from sediment sample from an anoxic zone, rich in decomposing organic material, obtained from the Tucuruí hydroelectric dam reservoir in eastern Brazilian Amazonia. The partial nucleotide sequences obtained were 83 to 95% similar to those available in databases, indicating a low diversity of archaeans in the reservoir. Two orders were identified -the Methanomicrobiales, and a unique Operational Taxonomic Unit (OTU) forming a clade with the Methanosarcinales according to low bootstrap values. Homology modeling was used to determine the three-dimensional (3D) structures, for this the partial nucleotide sequence of the mcrα were isolated and translated on their partial amino acid sequences. The 3D structures of the archaean mcrα observed in the present study varied little, and presented approximately 70% identity in comparison with the mcrα of Methanopyrus klanderi. The results demonstrated that the community of methanogenic archaeans of the anoxic C1 region of the Tucurui reservoir is relatively homogeneous. 2013-08-08T14:00:43Z 2013-08-08T14:00:43Z 2012-01 Artigo de Periódico SANTANA, Priscila Bessa, et al. Diversity and three-dimensional structures of the alpha Mcr of the methanogenic Archaea from the anoxic region of Tucuruí Lake, in Eastern Brazilian Amazonia. Genetics and Molecular Biology, São Paulo, v. 35, n. 1, p. 126-133, 2012, Epub 20 jan. 2012. Disponível em: <http://www.scielo.br/pdf/gmb/v35n1/2011-055.pdf>. Acesso em: 06 ago. 2013. <http://dx.doi.org/10.1590/S1415-47572012005000004>. 1415-4757 http://repositorio.ufpa.br/jspui/handle/2011/4094 eng Acesso Aberto application/pdf
institution	Repositório Institucional - Universidade Federal do Pará
collection	RI-UFPA
language	eng
topic	Archaea Metil-coenzima M redutase Modelagem por homologia Lago de Tucuruí - PA Tucuruí - PA Pará - Estado Amazônia brasileira
spellingShingle	Archaea Metil-coenzima M redutase Modelagem por homologia Lago de Tucuruí - PA Tucuruí - PA Pará - Estado Amazônia brasileira SANTANA, Priscila di Paula Bessa Diversity and three-dimensional structures of the alpha Mcr of the methanogenic Archaea from the anoxic region of Tucuruí Lake, in Eastern Brazilian Amazonia
topic_facet	Archaea Metil-coenzima M redutase Modelagem por homologia Lago de Tucuruí - PA Tucuruí - PA Pará - Estado Amazônia brasileira
description	Methanogenic archaeans are organisms of considerable ecological and biotechnological interest that produce methane through a restricted metabolic pathway, which culminates in the reaction catalyzed by the Methyl-coenzyme M reductase (Mcr) enzyme, and results in the release of methane. Using a metagenomic approach, the gene of the a subunit of mcr (mcrα) was isolated from sediment sample from an anoxic zone, rich in decomposing organic material, obtained from the Tucuruí hydroelectric dam reservoir in eastern Brazilian Amazonia. The partial nucleotide sequences obtained were 83 to 95% similar to those available in databases, indicating a low diversity of archaeans in the reservoir. Two orders were identified -the Methanomicrobiales, and a unique Operational Taxonomic Unit (OTU) forming a clade with the Methanosarcinales according to low bootstrap values. Homology modeling was used to determine the three-dimensional (3D) structures, for this the partial nucleotide sequence of the mcrα were isolated and translated on their partial amino acid sequences. The 3D structures of the archaean mcrα observed in the present study varied little, and presented approximately 70% identity in comparison with the mcrα of Methanopyrus klanderi. The results demonstrated that the community of methanogenic archaeans of the anoxic C1 region of the Tucurui reservoir is relatively homogeneous.
format	Artigo
author	SANTANA, Priscila di Paula Bessa
author2	GHILARDI JUNIOR, Rubens ALVES, Claudio Nahum SILVA, Jeronimo Lameira MCCULLOCH, John Anthony SCHNEIDER, Maria Paula Cruz SILVA, Artur Luiz da Costa da
author2Str	GHILARDI JUNIOR, Rubens ALVES, Claudio Nahum SILVA, Jeronimo Lameira MCCULLOCH, John Anthony SCHNEIDER, Maria Paula Cruz SILVA, Artur Luiz da Costa da
title	Diversity and three-dimensional structures of the alpha Mcr of the methanogenic Archaea from the anoxic region of Tucuruí Lake, in Eastern Brazilian Amazonia
title_short	Diversity and three-dimensional structures of the alpha Mcr of the methanogenic Archaea from the anoxic region of Tucuruí Lake, in Eastern Brazilian Amazonia
title_full	Diversity and three-dimensional structures of the alpha Mcr of the methanogenic Archaea from the anoxic region of Tucuruí Lake, in Eastern Brazilian Amazonia
title_fullStr	Diversity and three-dimensional structures of the alpha Mcr of the methanogenic Archaea from the anoxic region of Tucuruí Lake, in Eastern Brazilian Amazonia
title_full_unstemmed	Diversity and three-dimensional structures of the alpha Mcr of the methanogenic Archaea from the anoxic region of Tucuruí Lake, in Eastern Brazilian Amazonia
title_sort	diversity and three-dimensional structures of the alpha mcr of the methanogenic archaea from the anoxic region of tucuruí lake, in eastern brazilian amazonia
publishDate	2013
url	http://repositorio.ufpa.br/jspui/handle/2011/4094
_version_	1832602199199318016
score	11.755432

Diversity and three-dimensional structures of the alpha Mcr of the methanogenic Archaea from the anoxic region of Tucuruí Lake, in Eastern Brazilian Amazonia

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