514 blood samples from beef cattle were examined of to find anti- M. avium (subsp.)
paratuberculosis antibodies, using commercial ELISA test kit. The animals from samples
were all compous from cross between Indicus x Taurus race, between male and female, with
two age class, over 36 months old and below 36 months old, from of the 23 municipality of
Para State. Also were collected 100 samples of small intestine and mesenteric lymph nodes of
bacteriological test through Ziehl-Neelsen method to detect the M. avium (subsp.)
paratuberculosis. Were at random collected in slaughterhouse of Belem. In ELISA test,
through these 514 samples, 182 (35,4%) were seropositive and 332 (64,6%) samples were
seronegative. All the middle region were found soropositives animals. The female with over
36 months old presented larger response test, of the 92,06% seropositives animals. Among
male the prevalency larger (76,79%,) were obtained between the most new, with below 36
months old. Of 100 samples stained through Ziehl-Neelsen method, not were found either
Mycobacterium spp.. The conclusion, is the biggest number of seropositive animals of anti-
M. avium (subsp.) paratuberculosis sign of disease is presented in all the regions studied,
watching the risks of this disease with the elaborate more covering stud ies about the
paratuberculosis with the appling of directs and indirects diagnosis methods.
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