Medicinal plants are widely used and culturally in the amazon to treat various
diseases.Much of these plants has not been scientifically invesgated especially on
the aspects related to biological activitie.The species selected in this work is
Vatairea guianensis, used in traditional medicine to treat skin infections such as
cutaneous mycoses. This study evaluated the antibacterial activity in vitro of
hydroethanolic extract, hexane, chloroform and methanol Vatairea guianensis seeds
by microdilution method for obtaining the minimum inhibitory concentration (MIC)
and minimum bactericidal concentration (MBC) against Gram positive
(Staphylococcus aureus and Enterecoccus faecalis) and Gram negative bacteria
(Pseudomonas aeruginosa and Salmonella sp). The study also evaluated the
healing activity on open wounds in rats by topical aplication of the hydroethanolic
extract, represented by G1 positive control (fibrinase), G2 negative control (saline)
and experimental groups( G3 dose 500mg/kg, G4 250mg/kg,G5 100mg/kg) for
seven days and the acute oral toxicity. Histological analysis of the healing process
was evaluated by means of conventional technique including color HE and
Picrossirius for observation of the inflammatory reaction histomorphological
characteristics and descriptive analysis of collagen deposition, respectively. All extracts showed antibacterial activity against all tested microorganisms with an MIC
ranging from 3,12μg/mL to 50μg/mL and MBC values 6,25μg/mL to 100μg/mL. In histological analysis, the hidroethanolic extract decreased the intensity of
inflammation in the groups G1 and G3, stimulates the synthesis of collagen type III
em G1, G3 and G4 and increased collagen synthesis in G2 and G5. The experiment
with the hydroethanolic extract obtained from seeds of Vatairea guianensis
appeared to slow down the healing process effect by preventing the formation of
hypertrophic scar, suggesting a modulatory effect by extract. The acute toxicity
evaluation in mouse revealed that the hydroethanlic extract showed low toxicity by
the oral proceedings.
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