The discovery and synthesis of antimicrobial comprise an element of paramount
importance to health, however some of these substances have become obsolete due
to the emergence of resistant microorganisms conventional therapy. Within the forms
of treatment, natural products are an inexhaustible source of substances, including
propolis, which is known worldwide due to its antimicrobial activity. The objective of
this study was the microbiological analysis of six propolis samples collected from
regions Prudentópolis - PR , at different times of deposit hive, with up to 40 days of
deposit (New Propolis) and another with over 180 days (Propolis old). The
antimicrobial activity was observed against Pseudomonas aeruginosa, Escherichia
coli, Staphylococcus aureus, Micrococcus luteus and Enterococcus faecalis through
testing microdilution plate with colorimetric assay using resazurin. At the end of the
tests gave values ranging from 0.38 to 0.68 mg/ml to MIC new propolis and 0.34 to
1.3 mg/ml for the old to the microorganisms S. aureus and M. luteus having values
close to both of these as well as the CBM between 0.38 to 1.62 and from 0.67 to 2.6
mg/ml, respectively. Similarly, for E. faecalis MIC values between 0.76 and 2.73
mg/ml for the new propolis and 2.72 and 1.34 mg/ml of the propolis old were made,
and MBC values of 1.50 to 3.07 and 2.68 to 3.11 mg/ml, respectively, for samples
1V, 2N and 5N 5V CBM not observed for the concentrations studied. Gram- negative
microorganisms were not sensitive to propolis. We conclude that the new propolis
showed better antimicrobial activity, especially against S. aureus and M. luteus.
However, the data also show that the values of MIC and MBC were very close
between the different propolis, which was not evident reasons why the old propolis is
discarded.
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