Determination of strains of Helicobacter pylori and of polymorphism in the interleukin-8 gene in patients with stomach cancer
CONTEXT: Gastric neoplasia is the second most common cause of death by cancer in the world and H. pylori is classified as a type I human carcinogen by the World Health Organization. However, despite the high prevalence of infection by H. pylori around the world, less than 3% of individuals carrying...
| Autor principal: | VINAGRE, Ruth Maria Dias Ferreira |
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| Outros Autores: | CORVELO, Tereza Cristina de Oliveira, ARNAUD, Vanda Catão, LEITE, Ana Claudia Klautau, BARILE, Katarine Antonia dos Santos, MARTINS, Luisa Caricio |
| Grau: | Artigo |
| Idioma: | eng |
| Publicado em: |
2015
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| Acesso em linha: |
http://repositorio.ufpa.br/jspui/handle/2011/6553 |
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ir-2011-6553 |
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ir-2011-65532018-02-07T18:30:29Z Determination of strains of Helicobacter pylori and of polymorphism in the interleukin-8 gene in patients with stomach cancer Determinação das cepas do Helicobacter pylori e do polimorfismo do gene da interleucina-8 em pacientes com câncer gástrico VINAGRE, Ruth Maria Dias Ferreira CORVELO, Tereza Cristina de Oliveira ARNAUD, Vanda Catão LEITE, Ana Claudia Klautau BARILE, Katarine Antonia dos Santos MARTINS, Luisa Caricio Neoplasias gástricas Helicobacter pylori Polimorfismo genético Interleucina CONTEXT: Gastric neoplasia is the second most common cause of death by cancer in the world and H. pylori is classified as a type I human carcinogen by the World Health Organization. However, despite the high prevalence of infection by H. pylori around the world, less than 3% of individuals carrying the bacteria develop gastric neoplasias. Such a fact indicates that evolution towards malignancy may be associated with bacterial factors in the host and the environment. OBJECTIVES: To investigate the association between polymorphism in the region promoting the IL-8 (-251) gene and the H. pylori genotype, based on the vacA alleles and the presence of the cagA gene, using clinical and histopathological data. METHODS: In a prospective study, a total of 102 patients with stomach cancer and 103 healthy volunteers were analysed. Polymorphism in interleukin 8 (-251) was determined by the PCR-restriction fragment length polymorphism reaction and sequencing. PCR was used for genotyping the vacA alleles and the cagA in the bacterial strains PCR. Gastric biopsies were histologically assessed. RESULTS: The H. pylori serology was positive for 101 (99%) of all patients analysed, and 98 (97%) of them were colonized by only one strain. In patients with monoinfection, 82 (84%) of the bacterial strains observed had the s1b/m1 genotype. The cagA gene was detected in 74 (73%) of patients infected by H. pylori. The presence of the cagA gene was demonstrated as associated with the presence of the s1b/m1 genotype of the vacA gene (P = 0.002). As for polymorphism in the interleukin 8 (-251) gene we observed that the AA (P = 0.026) and AT (P = 0.005) genotypes were most frequent in the group of patients with gastric adenocarcinoma. By comparing the different types of isolated bacterial strains with the interleukin -8 (-251) and the histopathological data we observed that carriers of the A allele (AT and AA) infected by virulent strains (m1s1 cagA+) demonstrated a greater risk of presenting a degree of inflammation (OR = 24.75 CI 95% 2.29-267.20 P = 0.004) and increased neutrophilic activity (OR = 28.71 CI 95% 2.62-314 P = 0.002) in the gastric mucosa. CONCLUSION: Our results demonstrate that the interaction between polymorphism in the interleukin -8 (-251) gene, particularly with carriers of the A allele and the infecting type of H. pylori strain (s1m1 cagA positive) performs an important function in development of gastric adenocarcinoma. CONTEXTO: A neoplasia gástrica é a segunda causa mais comum de morte por câncer no mundo e o H. pylori é classificado como carcinógeno humano tipo I pela Organização Mundial de Saúde. Entretanto, apesar da elevada prevalência da infecção pelo H. pylori em todo mundo, menos de 3% de indivíduos portadores dessa bactéria desenvolvem neoplasias gástricas. Tal fato indica que a evolução para malignização possa estar associada a fatores bacterianos, do hospedeiro e do ambiente. OBJETIVOS: Investigou-se a associação do polimorfismo da região promotora do gene IL-8 (-251) e do genótipo do H. pylori, baseado nos alelos vacA e na presença do gene cagA, com a clínica e os dados histopatológicos. MÉTODOS: Em estudo prospectivo, 102 pacientes com câncer gástrico e 103 voluntários saudáveis foram analisados. O polimorfismo da IL-8 (-251) foi determinado pela reação de PCR-RFLP e sequenciamento. Para genotipagem dos alelos vacA e do gene cagA das cepas bacterianas foi utilizada a PCR. As biopsias gástricas foram avaliadas histologicamente. RESULTADOS: A sorologia para o H. pylori foi positiva em 101 (99%) de todos os pacientes analisados, e 98 (97%) deles foram colonizados por apenas uma cepa bacteriana. Em pacientes com monoinfecção, 82 (84%) das cepas bacterianas observadas apresentavam o genótipo s1b/m1. O gene cagA foi detectado em 74 (73%) dos pacientes infectados pelo H. pylori. A presença do gene cagA demonstrou estar associada com a presença do genótipo s1b/m1 do gene vacA (P = 0,002). Quanto ao polimorfismo do gene da IL-8 (-251), observou-se que os genótipos AA (P = 0,026) e AT (P = 0,005) foram mais frequentes no grupo de pacientes com adenocarcinoma gástrico. Comparando os diferentes tipos de cepas bacterianas isoladas, com o polimorfismo do gene da IL-8-251 e dados histopatológicos, observou-se que, portadores do alelo A (AT e AA) infectados por cepas virulentas (m1s1 cagA+), demonstraram risco aumentado de apresentar maior grau de inflamação (OR = 24,75 IC 95% 2,29-267,20 P = 0,004) e aumento da atividade neutrofílica (OR = 28,71 IC 95% 2.62-314 P = 0,002) na mucosa gástrica. CONCLUSÃO: Os resultados demonstram que a interação entre o polimorfismo do gene da IL-8, particularmente em portadores do alelo A, e o tipo de cepa infectante do H. pylori (s1m1 cagA positiva) desempenha importante função no desenvolvimento do câncer gástrico. 2015-04-17T17:17:44Z 2015-04-17T17:17:44Z 2011 Artigo de Periódico VINAGRE, Ruth Maria Dias Ferreira et al. Determination of strains of Helicobacter pylori and of polymorphism in the interleukin-8 gene in patients with stomach cancer. Arquivos de Gastroenterologia, São Paulo, v. 48, n. 1, p. 46-51, jan./mar. 2011. Disponível em: <http://www.scielo.br/pdf/ag/v48n1/a10v48n1.pdf>. Acesso em: 16 abr. 2015. <http://dx.doi.org/10.1590/S0004-28032011000100010>. 0004-2803 http://repositorio.ufpa.br/jspui/handle/2011/6553 eng Acesso Aberto application/pdf |
| institution |
Repositório Institucional - Universidade Federal do Pará |
| collection |
RI-UFPA |
| language |
eng |
| topic |
Neoplasias gástricas Helicobacter pylori Polimorfismo genético Interleucina |
| spellingShingle |
Neoplasias gástricas Helicobacter pylori Polimorfismo genético Interleucina VINAGRE, Ruth Maria Dias Ferreira Determination of strains of Helicobacter pylori and of polymorphism in the interleukin-8 gene in patients with stomach cancer |
| topic_facet |
Neoplasias gástricas Helicobacter pylori Polimorfismo genético Interleucina |
| description |
CONTEXT: Gastric neoplasia is the second most common cause of death by cancer in the world and H. pylori is classified as a type I human carcinogen by the World Health Organization. However, despite the high prevalence of infection by H. pylori around the world, less than 3% of individuals carrying the bacteria develop gastric neoplasias. Such a fact indicates that evolution towards malignancy may be associated with bacterial factors in the host and the environment.
OBJECTIVES: To investigate the association between polymorphism in the region promoting the IL-8 (-251) gene and the H. pylori genotype, based on the vacA alleles and the presence of the cagA gene, using clinical and histopathological data.
METHODS: In a prospective study, a total of 102 patients with stomach cancer and 103 healthy volunteers were analysed. Polymorphism in interleukin 8 (-251) was determined by the PCR-restriction fragment length polymorphism reaction and sequencing. PCR was used for genotyping the vacA alleles and the cagA in the bacterial strains PCR. Gastric biopsies were histologically assessed.
RESULTS: The H. pylori serology was positive for 101 (99%) of all patients analysed, and 98 (97%) of them were colonized by only one strain. In patients with monoinfection, 82 (84%) of the bacterial strains observed had the s1b/m1 genotype. The cagA gene was detected in 74 (73%) of patients infected by H. pylori. The presence of the cagA gene was demonstrated as associated with the presence of the s1b/m1 genotype of the vacA gene (P = 0.002). As for polymorphism in the interleukin 8 (-251) gene we observed that the AA (P = 0.026) and AT (P = 0.005) genotypes were most frequent in the group of patients with gastric adenocarcinoma. By comparing the different types of isolated bacterial strains with the interleukin -8 (-251) and the histopathological data we observed that carriers of the A allele (AT and AA) infected by virulent strains (m1s1 cagA+) demonstrated a greater risk of presenting a degree of inflammation (OR = 24.75 CI 95% 2.29-267.20 P = 0.004) and increased neutrophilic activity (OR = 28.71 CI 95% 2.62-314 P = 0.002) in the gastric mucosa.
CONCLUSION: Our results demonstrate that the interaction between polymorphism in the interleukin -8 (-251) gene, particularly with carriers of the A allele and the infecting type of H. pylori strain (s1m1 cagA positive) performs an important function in development of gastric adenocarcinoma. |
| format |
Artigo |
| author |
VINAGRE, Ruth Maria Dias Ferreira |
| author2 |
CORVELO, Tereza Cristina de Oliveira ARNAUD, Vanda Catão LEITE, Ana Claudia Klautau BARILE, Katarine Antonia dos Santos MARTINS, Luisa Caricio |
| author2Str |
CORVELO, Tereza Cristina de Oliveira ARNAUD, Vanda Catão LEITE, Ana Claudia Klautau BARILE, Katarine Antonia dos Santos MARTINS, Luisa Caricio |
| title |
Determination of strains of Helicobacter pylori and of polymorphism in the interleukin-8 gene in patients with stomach cancer |
| title_short |
Determination of strains of Helicobacter pylori and of polymorphism in the interleukin-8 gene in patients with stomach cancer |
| title_full |
Determination of strains of Helicobacter pylori and of polymorphism in the interleukin-8 gene in patients with stomach cancer |
| title_fullStr |
Determination of strains of Helicobacter pylori and of polymorphism in the interleukin-8 gene in patients with stomach cancer |
| title_full_unstemmed |
Determination of strains of Helicobacter pylori and of polymorphism in the interleukin-8 gene in patients with stomach cancer |
| title_sort |
determination of strains of helicobacter pylori and of polymorphism in the interleukin-8 gene in patients with stomach cancer |
| publishDate |
2015 |
| url |
http://repositorio.ufpa.br/jspui/handle/2011/6553 |
| _version_ |
1832602237951541248 |
| score |
11.755432 |