Beta-lactamases are produced by gram-positive and gram-negative bacteria. About 40% to 50% of women developed a urinary tract infection during their adult life. The aim of the present study was the characterization of genes Bla SHV, Bla TEM e Bla CTX-M beta-lactamase-producing extended-spectrum E. coli and Klebsiella spp isolated from pregnant women with urinary tract infection treated at a Basic Health Unit of Empress - MA from May to August 2012. The study included 50 pregnant women over 18 years who presented with symptoms clinical characterization of urinary tract infection (UTI), referenced in the clinic in the Basic Health Empress - MA. Urine was collected for urine culture and sensitivity of achievement, subsequently was performed Polymerase Chain Reaction (PCR) to detect the presence of genes Bla SHV, Bla TEM e Bla CTX-M, enzymes producing beta-lactamases. The mean age of these patients was 21 years, 42% being aged 18 to 25 years, 70% of these fixed address in Empress and the other 30% come from other municipalities. Among these, 24% of the volunteers already had UTI during pregnancy and pregnancy out of state and 80% of them reported using antibiotics without prescription. Of the 12 samples with positive microbiological growth, 11 were positive for Escherichia coli with the antibiogram results that were resistant to the antibiotic cephalothin at 91.7%. Being isolated, a strain of Klebsiella spp, and this was resistant to all antibiotics tested. By analyzing the results of PCR samples isolated three genes Bla SHV, Bla TEM e Bla CTX-M, were not observed in samples P9, P11 and P12, but in the other samples was the occurrence of at least one of the genes. This study confirmed the presence of three types of genes (Bla SHV, Bla TEM e Bla CTX-M) among the samples studied.
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