Diagnóstico de paratuberculose por biópsia retal em búfalos
Paratuberculosis in a herd of buffaloes was studied in the municipality of São Mateus, Maranhão, Brazil. Rectal biopsies were performed in 140 male and female Murrah, Mediterranean and crossbreed buffaloes older than 3 years. Postmortem examination of 11 buffaloes was performed to compare the rectal...
| Autor principal: | REIS, Alessandra dos Santos Belo |
|---|---|
| Outros Autores: | BRITO, Marilene de Farias, BEZERRA JÚNIOR, Pedro Soares, FONSECA JÚNIOR, Antônio Augusto, UBIALI, Daniel Guimarães, MOTA, Rinaldo Aparecido, LEITE, Rômulo Cerqueira, BARBOSA NETO, José Diomedes |
| Grau: | Artigo |
| Idioma: | por |
| Publicado em: |
2016
|
| Assuntos: | |
| Acesso em linha: |
http://repositorio.ufpa.br/jspui/handle/2011/7053 |
| id |
ir-2011-7053 |
|---|---|
| recordtype |
dspace |
| spelling |
ir-2011-70532018-02-22T18:02:12Z Diagnóstico de paratuberculose por biópsia retal em búfalos Rectal biopsy for the diagnosis of paratuberculosis in buffaloes REIS, Alessandra dos Santos Belo BRITO, Marilene de Farias BEZERRA JÚNIOR, Pedro Soares FONSECA JÚNIOR, Antônio Augusto UBIALI, Daniel Guimarães MOTA, Rinaldo Aparecido LEITE, Rômulo Cerqueira BARBOSA NETO, José Diomedes Diagnóstico veterinário Paratuberculose Doença de Johne Biópsia retal Búfalo São Mateus - Ma Maranhão - Estado Paratuberculosis in a herd of buffaloes was studied in the municipality of São Mateus, Maranhão, Brazil. Rectal biopsies were performed in 140 male and female Murrah, Mediterranean and crossbreed buffaloes older than 3 years. Postmortem examination of 11 buffaloes was performed to compare the rectal biopsies with possible lesions in mesenteric nodes and the intestine. The history of the herd and clinical examination revealed progressive weight loss and non-responsive antimicrobial diarrhea, dehydration and submandibular edema. Rectal biopsies showed in six buffaloes microscopically suggestive lesions for paratuberculosis through hematoxilin-eosin staining (HE), characterized by moderate multifocal granulomatous enteritis with epithelioid cell infiltration. In four buffaloes Langhans giant cells were found. In 15 buffaloes lymphocytic infiltrate was observed in the lamina propria of the large intestine. Ziehl-Neelsen staining (ZN) revealed in 4.3% (6/140) acid-fast bacilli in the rectal mucosa. Real time PCR amplified to 5.71% (7/140) Mycobacterium avium subsp. paratuberculosis (Map) DNA. 11 buffalos were submitted to postmortem examination, gross examination revealed augmented mesenteric nodes with whitish areas in the cut surface. The mucosa of the small intestine was irregular and thickened, with evident traverse folds and Peyer plates. The brownish intestinal content was fluid, the ileocecal valve area thickened and edematous with evident lymphatic vessels. Histological lesions in the mesenteric lymph node and small intestine four buffalo were compatible with those already described in the literature, and presented acid-fast bacilli by ZN staining and amplification of Map genetic material in qPCR. The concordance between the rectal biopsy and the postmortem samples was in agreement with the Kappa test (K=0.792) and was considered substantial or high. The rectal biopsy showed to be promising and can be used by practitioners, together with other techniques, for antemortem diagnosis in buffalo herds suspected to be affected by paratuberculosis. Samples obtained by rectal biopsy were suitable for ZN staining and real time PCR. Rectal biopsy can be used as a tool for diagnosis and control of Johne´s disease in buffaloes to select and eliminate positive animals within the herd and decrease gradually the spread of Map. Foram realizadas biópsias retais de 140 búfalos, machos e fêmeas, das raças Murrah e mestiços de Murrah com Mediterrâneo, com idade acima de três anos, em uma propriedade no município de São Mateus, Maranhão, Brasil. Adicionalmente foram realizadas necropsias de 11 búfalos, para realizar um estudo comparativo entre os achados das biópsias retais e de tecidos de íleo e linfonodo mesentérico. A propriedade apresentava histórico de animais com emagrecimento progressivo e diarreia não responsiva a antimicrobianos. Os búfalos apresentavam sinais clínicos caracterizados por diarreia, estado nutricional regular a ruim, desidratação e edema submandibular. Nas biópsias retais seis búfalos apresentaram lesões sugestivas da paratuberculose na Hematoxilina-Eosina (HE), sendo estas caracterizadas por inflamação granulomatosa multifocal moderada na lâmina própria com macrófagos epitelioides. Em quatro animais foram observadas adicionalmente células gigantes do tipo Langhans. Em 15 búfalos foi observado infiltrado linfocitário multifocal leve na lâmina própria. Pela coloração de Ziehl-Neelsen (ZN), 4,3% (6/140) apresentaram bacilos álcool-ácido resistentes (BAAR) e na PCR em tempo real (qPCR), 5,71% (7/140) tiveram amplificação do material genético. Foram necropsiados 11 búfalos, à necropsia foram observados aumento de linfonodos mesentéricos com áreas esbranquiçadas na superfície de corte; intestino delgado e grosso com dobras transversais evidentes, mucosa espessada e irregular, de aspecto reticulado, placas de Peyer evidentes e conteúdo líquido e marrom. Ainda se viam áreas espessadas em torno da válvula ileocecal e vasos linfáticos evidentes. As lesões histológicas localizadas no intestino delgado e linfonodos mesentéricos de quatro búfalos foram compatíveis com lesões já descritas na literatura, e apresentaram BAAR e amplificação de material genético na qPCR. A concordância entre a biópsia retal e a análise dos tecidos de íleo e linfonodo mesentérico, segundo o teste Kappa (K=0,792), foi alta. A biópsia retal realizada demonstrou ser promissora e pode ser empregada, juntamente com outras técnicas, para auxiliar no diagnóstico ante mortem em búfalos de rebanhos com suspeita de paratuberculose; pela mesma foi possível detectar animais positivos através da coloração de ZN e qPCR. Os resultados obtidos podem ser utilizados no controle da enfermidade para selecionar e eliminar animais positivos do rebanho, diminuindo gradualmente, a disseminação do agente no ambiente, e a consequente contaminação de outros animais. 2016-01-18T15:09:04Z 2016-01-18T15:09:04Z 2015-10 Artigo de Periódico REIS, Alessandra dos Santos Belo et al. Diagnóstico de paratuberculose por biópsia retal em búfalos. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 35, n. 10, p. 823-828, out. 2015. Disponível em: <http://www.scielo.br/pdf/pvb/v35n10/1678-5150-pvb-35-10-00823.pdf>. Acesso em: 18 jan. 2016. <http://dx.doi.org/10.1590/S0100-736X2015001000001>. 1678-5150 http://repositorio.ufpa.br/jspui/handle/2011/7053 por Acesso Aberto application/pdf |
| institution |
Repositório Institucional - Universidade Federal do Pará |
| collection |
RI-UFPA |
| language |
por |
| topic |
Diagnóstico veterinário Paratuberculose Doença de Johne Biópsia retal Búfalo São Mateus - Ma Maranhão - Estado |
| spellingShingle |
Diagnóstico veterinário Paratuberculose Doença de Johne Biópsia retal Búfalo São Mateus - Ma Maranhão - Estado REIS, Alessandra dos Santos Belo Diagnóstico de paratuberculose por biópsia retal em búfalos |
| topic_facet |
Diagnóstico veterinário Paratuberculose Doença de Johne Biópsia retal Búfalo São Mateus - Ma Maranhão - Estado |
| description |
Paratuberculosis in a herd of buffaloes was studied in the municipality of São Mateus, Maranhão, Brazil. Rectal biopsies were performed in 140 male and female Murrah, Mediterranean and crossbreed buffaloes older than 3 years. Postmortem examination of 11 buffaloes was performed to compare the rectal biopsies with possible lesions in mesenteric nodes and the intestine. The history of the herd and clinical examination revealed progressive weight loss and non-responsive antimicrobial diarrhea, dehydration and submandibular edema. Rectal biopsies showed in six buffaloes microscopically suggestive lesions for paratuberculosis through hematoxilin-eosin staining (HE), characterized by moderate multifocal granulomatous enteritis with epithelioid cell infiltration. In four buffaloes Langhans giant cells were found. In 15 buffaloes lymphocytic infiltrate was observed in the lamina propria of the large intestine. Ziehl-Neelsen staining (ZN) revealed in 4.3% (6/140) acid-fast bacilli in the rectal mucosa. Real time PCR amplified to 5.71% (7/140) Mycobacterium avium subsp. paratuberculosis (Map) DNA. 11 buffalos were submitted to postmortem examination, gross examination revealed augmented mesenteric nodes with whitish areas in the cut surface. The mucosa of the small intestine was irregular and thickened, with evident traverse folds and Peyer plates. The brownish intestinal content was fluid, the ileocecal valve area thickened and edematous with evident lymphatic vessels. Histological lesions in the mesenteric lymph node and small intestine four buffalo were compatible with those already described in the literature, and presented acid-fast bacilli by ZN staining and amplification of Map genetic material in qPCR. The concordance between the rectal biopsy and the postmortem samples was in agreement with the Kappa test (K=0.792) and was considered substantial or high. The rectal biopsy showed to be promising and can be used by practitioners, together with other techniques, for antemortem diagnosis in buffalo herds suspected to be affected by paratuberculosis. Samples obtained by rectal biopsy were suitable for ZN staining and real time PCR. Rectal biopsy can be used as a tool for diagnosis and control of Johne´s disease in buffaloes to select and eliminate positive animals within the herd and decrease gradually the spread of Map. |
| format |
Artigo |
| author |
REIS, Alessandra dos Santos Belo |
| author2 |
BRITO, Marilene de Farias BEZERRA JÚNIOR, Pedro Soares FONSECA JÚNIOR, Antônio Augusto UBIALI, Daniel Guimarães MOTA, Rinaldo Aparecido LEITE, Rômulo Cerqueira BARBOSA NETO, José Diomedes |
| author2Str |
BRITO, Marilene de Farias BEZERRA JÚNIOR, Pedro Soares FONSECA JÚNIOR, Antônio Augusto UBIALI, Daniel Guimarães MOTA, Rinaldo Aparecido LEITE, Rômulo Cerqueira BARBOSA NETO, José Diomedes |
| title |
Diagnóstico de paratuberculose por biópsia retal em búfalos |
| title_short |
Diagnóstico de paratuberculose por biópsia retal em búfalos |
| title_full |
Diagnóstico de paratuberculose por biópsia retal em búfalos |
| title_fullStr |
Diagnóstico de paratuberculose por biópsia retal em búfalos |
| title_full_unstemmed |
Diagnóstico de paratuberculose por biópsia retal em búfalos |
| title_sort |
diagnóstico de paratuberculose por biópsia retal em búfalos |
| publishDate |
2016 |
| url |
http://repositorio.ufpa.br/jspui/handle/2011/7053 |
| _version_ |
1787149362526158848 |
| score |
11.674752 |