Helicobacter pylori infection in patients with different gastrointestinal diseases from northern brazil
Background - The mechanisms whereby Helicobacter pylori produces different pathological manifestations in the stomach and duodenum are not fully understood. Considering the geographic diversity in the prevalence of virulence factors of this microorganism and their association with the development of...
| Autor principal: | VINAGRE, Igor Dias Ferreira |
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| Outros Autores: | QUEIROZ, André Lima de, SILVA JÚNIOR, Mário Ribeiro da, VINAGRE, Ruth Maria Dias Ferreira, MARTINS, Luisa Caricio |
| Grau: | Artigo |
| Idioma: | por |
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Instituto Brasileiro de Estudos e Pesquisas de Gastroenterologia e Outras Especialidades
2017
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http://repositorio.ufpa.br/jspui/handle/2011/7940 http://dx.doi.org/10.1590/S0004-28032015000400004 |
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ir-2011-79402019-09-30T12:18:22Z Helicobacter pylori infection in patients with different gastrointestinal diseases from northern brazil VINAGRE, Igor Dias Ferreira QUEIROZ, André Lima de SILVA JÚNIOR, Mário Ribeiro da VINAGRE, Ruth Maria Dias Ferreira MARTINS, Luisa Caricio Helicobacter pylori Patologia Infecção Doenças gastrointestinais Genótipo Análise histopatológica Hospital Ophir Loyola Helicobacter infections Gastrointestinal diseases Genotype Background - The mechanisms whereby Helicobacter pylori produces different pathological manifestations in the stomach and duodenum are not fully understood. Considering the geographic diversity in the prevalence of virulence factors of this microorganism and their association with the development of different diseases, the search for pathogenicity markers such as CagA and VacA alleles by molecular techniques has intensified. Objectives - To investigate the presence of H. pylori infection and the frequency of different genotypes of this bacterium in patients with gastrointestinal diseases from Northern Brazil, and to establish their association with the histopathological findings. Methods - In a prospective study, samples were collected from 554 patients with different gastrointestinal diseases (gastritis, duodenal ulcer, gastric ulcer, and gastric cancer) seen at a referral hospital attending the entire State of Pará, located in the metropolitan region of Belém. Data such as gender and age obtained with an epidemiological questionnaire were analyzed. The presence of H. pylori and the bacterial genotype were investigated by PCR. Gastric biopsies were assessed histologically. Results - The prevalence of H. pylori infection was 91%. Infection was more frequent among patients with gastric ulcer and gastric cancer. In these groups, there was a predominance of men and older patients when compared to the other two groups studied. The predominant bacterial genotype was s1m1cagA+, which was more frequent among patients with gastric ulcer, duodenal ulcer and gastric cancer. A significant association was observed between s1m1cagA+ strains and a higher degree of inflammation, neutrophil activity and development of intestinal metaplasia. Conclusion - The present study demonstrates a high incidence of H. pylori infection in the patients analyzed, especially among those with gastric ulcer and gastric cancer. Virulent s1m- 1cagA+ strains predominated and were associated with more severe lesions. Contexto - Os mecanismos pelos quais o H. pylori produz diferentes quadros patológicos no estômago e no duodeno não são totalmente conhecidos. Considerando a diversidade geográfica relacionada à prevalência dos fatores de virulência desse microrganismo e sua associação com o desenvolvimento de diferentes doenças, vem se intensificando a pesquisa de marcadores de patogenicidade, como o CagA e os alelos do VacA por técnicas moleculares. Objetivos - O objetivo desse estudo foi investigar a presença da infecção por H. pylori, e a frequência dos diferentes genótipos dessa bactéria em pacientes com doenças gastrointestinais da nossa região, procurando estabelecer sua associação com os achados histopatológicos. Métodos - Em estudo prospectivo, foram coletadas amostras de 554 pacientes com diferentes doenças gastrointestinais (gastrite, úlcera duodenal, úlcera gástrica e câncer gástrico), atendidos em hospital de referência para todo o Estado do Pará, localizado na região metropolitana de Belém. Foram analisados dados obtidos através de questionário epidemiológico, relacionados ao sexo e faixa etária desses pacientes. A presença do H. pylori e do genótipo bacteriano foi detectada utilizando a PCR. As biopsias gástricas foram avaliadas histologicamente. Resultados - Observou-se uma prevalência de 91% da infecção pelo H. pylori, sendo mais frequente nos portadores de úlcera gástrica e câncer gástrico, nos quais houve predomínio do sexo masculino e a idade foi maior que a dos outros dois grupos estudados. O genótipo bacteriano predominante foi o s1m1cagA positivo, sendo mais frequentes entre os pacientes com úlcera gástrica, úlcera duodenal e câncer gástrico. Houve associação significante das cepas com o genótipo s1m1cagA positivo com maior grau de inflamação, atividade neutrofílica e desenvolvimento de metaplasia intestinal. Conclusão - Nosso estudo demonstra a alta incidência da infecção pelo H. pylori nos pacientes analisados em nosso meio, especialmente em portadores de úlcera e câncer gástricos. As cepas virulentas s1m1cagA+ foram predominantes e estavam associadas a lesões mais graves. VINAGRE, I. D. F.; MARTINS, L. C. Universidade Federal do Pará 2017-03-21T14:05:14Z 2017-03-21T14:05:14Z 2015-12 Artigo de Periódico VINAGRE, Igor Dias Ferreira et al. Helicobacter pylori infection in patients with different gastrointestinal diseases from northern Brazil. Arquivos de Gastroenterologia, São Paulo, v. 52, n. 4, p. 266-271, Oct./Dec. 2015. DOI: http://dx.doi.org/10.1590/S0004-28032015000400004. Disponível em: http://repositorio.ufpa.br/jspui/handle/2011/10941. Acesso em: . 1678-4219 http://repositorio.ufpa.br/jspui/handle/2011/7940 http://dx.doi.org/10.1590/S0004-28032015000400004 por Arquivos de Gastroenterologia Acesso Aberto application/pdf Instituto Brasileiro de Estudos e Pesquisas de Gastroenterologia e Outras Especialidades Brasil IBEPEGE http://ref.scielo.org/f5kjf8 |
| institution |
Repositório Institucional - Universidade Federal do Pará |
| collection |
RI-UFPA |
| language |
por |
| topic |
Helicobacter pylori Patologia Infecção Doenças gastrointestinais Genótipo Análise histopatológica Hospital Ophir Loyola Helicobacter infections Gastrointestinal diseases Genotype |
| spellingShingle |
Helicobacter pylori Patologia Infecção Doenças gastrointestinais Genótipo Análise histopatológica Hospital Ophir Loyola Helicobacter infections Gastrointestinal diseases Genotype VINAGRE, Igor Dias Ferreira Helicobacter pylori infection in patients with different gastrointestinal diseases from northern brazil |
| topic_facet |
Helicobacter pylori Patologia Infecção Doenças gastrointestinais Genótipo Análise histopatológica Hospital Ophir Loyola Helicobacter infections Gastrointestinal diseases Genotype |
| description |
Background - The mechanisms whereby Helicobacter pylori produces different pathological manifestations in the stomach and duodenum are not fully understood. Considering the geographic diversity in the prevalence of virulence factors of this microorganism and their association with the development of different diseases, the search for pathogenicity markers such as CagA and VacA alleles by molecular techniques has intensified. Objectives - To investigate the presence of H. pylori infection and the frequency of different genotypes of this bacterium in patients with gastrointestinal diseases from Northern Brazil, and to establish their association with the histopathological findings. Methods - In a prospective study, samples were collected from 554 patients with different gastrointestinal diseases (gastritis, duodenal ulcer, gastric ulcer, and gastric cancer) seen at a referral hospital attending the
entire State of Pará, located in the metropolitan region of Belém. Data such as gender and age obtained with an epidemiological questionnaire were analyzed. The presence of H. pylori and the bacterial genotype were investigated by PCR. Gastric biopsies were assessed histologically. Results - The prevalence of H. pylori infection was 91%. Infection was more frequent among patients with
gastric ulcer and gastric cancer. In these groups, there was a predominance of men and older patients when compared to the other two groups studied. The predominant bacterial genotype was s1m1cagA+, which was more frequent among patients with gastric ulcer, duodenal ulcer and gastric cancer. A significant association was observed between s1m1cagA+ strains and a higher degree
of inflammation, neutrophil activity and development of intestinal metaplasia. Conclusion - The present study demonstrates a high incidence of H. pylori infection in the patients analyzed, especially among those with gastric ulcer and gastric cancer. Virulent s1m- 1cagA+ strains predominated and were associated with more severe lesions. |
| format |
Artigo |
| author |
VINAGRE, Igor Dias Ferreira |
| author2 |
QUEIROZ, André Lima de SILVA JÚNIOR, Mário Ribeiro da VINAGRE, Ruth Maria Dias Ferreira MARTINS, Luisa Caricio |
| author2Str |
QUEIROZ, André Lima de SILVA JÚNIOR, Mário Ribeiro da VINAGRE, Ruth Maria Dias Ferreira MARTINS, Luisa Caricio |
| title |
Helicobacter pylori infection in patients with different gastrointestinal diseases from northern brazil |
| title_short |
Helicobacter pylori infection in patients with different gastrointestinal diseases from northern brazil |
| title_full |
Helicobacter pylori infection in patients with different gastrointestinal diseases from northern brazil |
| title_fullStr |
Helicobacter pylori infection in patients with different gastrointestinal diseases from northern brazil |
| title_full_unstemmed |
Helicobacter pylori infection in patients with different gastrointestinal diseases from northern brazil |
| title_sort |
helicobacter pylori infection in patients with different gastrointestinal diseases from northern brazil |
| publisher |
Instituto Brasileiro de Estudos e Pesquisas de Gastroenterologia e Outras Especialidades |
| publishDate |
2017 |
| url |
http://repositorio.ufpa.br/jspui/handle/2011/7940 http://dx.doi.org/10.1590/S0004-28032015000400004 |
| _version_ |
1832604888843943936 |
| score |
11.755432 |