Local inhibition of nitrergic activity in tenotomized rats accelerates muscle regeneration by increasing fiber area and decreasing central core lesions
Muscular atrophy is a progressive degeneration characterized by muscular proteolysis, loss of mass and decrease in fiber area. Tendon rupture induces muscular atrophy due to an intrinsic functional connection. Local inhibition of nitric oxide synthase (NOS) by No-nitro-L-arginine methyl ester (L-N...
| Autor principal: | SEABRA, Aline Damasceno |
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| Outros Autores: | MORAES, Suellen Alessandra Soares de, BATISTA, Evander de Jesus Oliveira, GARCIA, Tarcyane Barata, SOUZA, Martha Costa de, OLIVEIRA, Karen Renata Matos, SILVA, Anderson Manoel Herculano Oliveira da |
| Grau: | Artigo |
| Idioma: | por |
| Publicado em: |
Universidade Federal do Pará
2017
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| Acesso em linha: |
http://repositorio.ufpa.br/jspui/handle/2011/8368 |
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ir-2011-8368 |
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ir-2011-83682017-10-16T12:27:21Z Local inhibition of nitrergic activity in tenotomized rats accelerates muscle regeneration by increasing fiber area and decreasing central core lesions SEABRA, Aline Damasceno MORAES, Suellen Alessandra Soares de BATISTA, Evander de Jesus Oliveira GARCIA, Tarcyane Barata SOUZA, Martha Costa de OLIVEIRA, Karen Renata Matos SILVA, Anderson Manoel Herculano Oliveira da Atrofia muscular Regeneração muscular Proteínas Histoquímica Tenotomia Fibra muscular Morfologia Muscular atrophy is a progressive degeneration characterized by muscular proteolysis, loss of mass and decrease in fiber area. Tendon rupture induces muscular atrophy due to an intrinsic functional connection. Local inhibition of nitric oxide synthase (NOS) by No-nitro-L-arginine methyl ester (L-NAME) accelerates tendon histological recovery and induces functional improvement. Here we evaluate the effects of such local nitrergic inhibition on the pattern of soleus muscle regeneration after tenotomy. Adult male Wistar rats (240 to 280 g) were divided into four experimental groups: control (n=4), tenotomized (n=6), vehicle (n=6), and L-NAME (n=6). Muscular atrophy was induced by calcaneal tendon rupture in rats. Changes in muscle wet weight and total protein levels were determined by the Bradford method, and muscle fiber area and central core lesion (CCL) occurrence were evaluated by histochemical assays. Compared to tenotomized (69.3±22%) and vehicle groups (68.1%±17%), L-NAME treatment induced an increase in total protein level (108.3±21%) after 21 days post-injury. A reduction in fiber areas was observed in tenotomized (56.3±1.3%) and vehicle groups (53.9±3.9%). However, L-NAME treatment caused an increase in this parameter (69.3±1.6%). Such events were preceded by a remarkable reduction in the number of fibers with CCL in L-NAME-treated animals (12±2%), but not in tenotomized (21±2.5%) and vehicle groups (19.6±2.8%). Altogether, our data reveal that inhibition of tendon NOS contributed to the attenuation of atrophy and acceleration of muscle regeneration. 2017-05-11T13:17:37Z 2017-05-11T13:17:37Z 2017-02 Artigo de Periódico SEABRA, Aline Damasceno et al. Local inhibition of nitrergic activity in tenotomized rats accelerates muscle regeneration by increasing fiber area and decreasing central core lesions. Brazilian Journal of Medical Biological Research, Ribeirão Preto, v. 50, n. 3, e5556, 2017. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000300601&lng=pt&nrm=iso>. Acesso em: 11 maio 2017. Epub 20-Fev-2017. <http://dx.doi.org/10.1590/1414-431x20165556>. 1414-431X http://repositorio.ufpa.br/jspui/handle/2011/8368 por Brazilian Journal of Medical and Biological Research Acesso Aberto application/pdf Universidade Federal do Pará Brasil UFPA |
| institution |
Repositório Institucional - Universidade Federal do Pará |
| collection |
RI-UFPA |
| language |
por |
| topic |
Atrofia muscular Regeneração muscular Proteínas Histoquímica Tenotomia Fibra muscular Morfologia |
| spellingShingle |
Atrofia muscular Regeneração muscular Proteínas Histoquímica Tenotomia Fibra muscular Morfologia SEABRA, Aline Damasceno Local inhibition of nitrergic activity in tenotomized rats accelerates muscle regeneration by increasing fiber area and decreasing central core lesions |
| topic_facet |
Atrofia muscular Regeneração muscular Proteínas Histoquímica Tenotomia Fibra muscular Morfologia |
| description |
Muscular atrophy is a progressive degeneration characterized by muscular proteolysis, loss of mass and decrease in fiber area.
Tendon rupture induces muscular atrophy due to an intrinsic functional connection. Local inhibition of nitric oxide synthase
(NOS) by No-nitro-L-arginine methyl ester (L-NAME) accelerates tendon histological recovery and induces functional improvement.
Here we evaluate the effects of such local nitrergic inhibition on the pattern of soleus muscle regeneration after tenotomy.
Adult male Wistar rats (240 to 280 g) were divided into four experimental groups: control (n=4), tenotomized (n=6), vehicle
(n=6), and L-NAME (n=6). Muscular atrophy was induced by calcaneal tendon rupture in rats. Changes in muscle wet weight
and total protein levels were determined by the Bradford method, and muscle fiber area and central core lesion (CCL) occurrence
were evaluated by histochemical assays. Compared to tenotomized (69.3±22%) and vehicle groups (68.1%±17%),
L-NAME treatment induced an increase in total protein level (108.3±21%) after 21 days post-injury. A reduction in fiber areas
was observed in tenotomized (56.3±1.3%) and vehicle groups (53.9±3.9%). However, L-NAME treatment caused an increase
in this parameter (69.3±1.6%). Such events were preceded by a remarkable reduction in the number of fibers with CCL in
L-NAME-treated animals (12±2%), but not in tenotomized (21±2.5%) and vehicle groups (19.6±2.8%). Altogether, our data
reveal that inhibition of tendon NOS contributed to the attenuation of atrophy and acceleration of muscle regeneration. |
| format |
Artigo |
| author |
SEABRA, Aline Damasceno |
| author2 |
MORAES, Suellen Alessandra Soares de BATISTA, Evander de Jesus Oliveira GARCIA, Tarcyane Barata SOUZA, Martha Costa de OLIVEIRA, Karen Renata Matos SILVA, Anderson Manoel Herculano Oliveira da |
| author2Str |
MORAES, Suellen Alessandra Soares de BATISTA, Evander de Jesus Oliveira GARCIA, Tarcyane Barata SOUZA, Martha Costa de OLIVEIRA, Karen Renata Matos SILVA, Anderson Manoel Herculano Oliveira da |
| title |
Local inhibition of nitrergic activity in tenotomized rats accelerates muscle regeneration by increasing fiber area and decreasing central core lesions |
| title_short |
Local inhibition of nitrergic activity in tenotomized rats accelerates muscle regeneration by increasing fiber area and decreasing central core lesions |
| title_full |
Local inhibition of nitrergic activity in tenotomized rats accelerates muscle regeneration by increasing fiber area and decreasing central core lesions |
| title_fullStr |
Local inhibition of nitrergic activity in tenotomized rats accelerates muscle regeneration by increasing fiber area and decreasing central core lesions |
| title_full_unstemmed |
Local inhibition of nitrergic activity in tenotomized rats accelerates muscle regeneration by increasing fiber area and decreasing central core lesions |
| title_sort |
local inhibition of nitrergic activity in tenotomized rats accelerates muscle regeneration by increasing fiber area and decreasing central core lesions |
| publisher |
Universidade Federal do Pará |
| publishDate |
2017 |
| url |
http://repositorio.ufpa.br/jspui/handle/2011/8368 |
| _version_ |
1832602852931928064 |
| score |
11.755432 |