Mannose-binding lectin 2 (Mbl2) gene polymorphisms are related to protein plasma levels, but not to heart disease and infection by Chlamydia
The presence of the single nucleotide polymorphisms in exon 1 of the mannose-binding lectin 2 (MBL2) gene was evaluated in a sample of 159 patients undergoing coronary artery bypass surgery (71 patients undergoing valve replacement surgery and 300 control subjects) to investigate a possible associat...
| Autor principal: | QUEIROZ, Maria Alice Freitas |
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| Outros Autores: | GOMES, Samara Tatielle Monteiro, ALMEIDA, Núbia Caroline Costa de, SOUSA, Maria Izete Machado de, COSTA, Suzanne Roberta Cardoso Fernandes, HERMES, Renata Bezerra, LIMA, Sandra Souza, ZANINOTTO, Marcelo Martins, FOSSA, Marco Antonio Ayin , MANESCYH, Manoel Araujo, FEITOSA, Rosimar Neris Martins, AZEVEDO, Vânia Nakauth, MACHADO, Luiz Fernando Almeida, ISHAK, Marluísa de Oliveira Guimarães, ISHAK, Ricardo, VALLINOTO, Antonio Carlos Rosário |
| Grau: | Artigo |
| Idioma: | por |
| Publicado em: |
Universidade Federal do Pará
2017
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| Acesso em linha: |
http://repositorio.ufpa.br/jspui/handle/2011/8375 |
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ir-2011-8375 |
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ir-2011-83752017-10-16T12:27:21Z Mannose-binding lectin 2 (Mbl2) gene polymorphisms are related to protein plasma levels, but not to heart disease and infection by Chlamydia QUEIROZ, Maria Alice Freitas GOMES, Samara Tatielle Monteiro ALMEIDA, Núbia Caroline Costa de SOUSA, Maria Izete Machado de COSTA, Suzanne Roberta Cardoso Fernandes HERMES, Renata Bezerra LIMA, Sandra Souza ZANINOTTO, Marcelo Martins FOSSA, Marco Antonio Ayin MANESCYH, Manoel Araujo FEITOSA, Rosimar Neris Martins AZEVEDO, Vânia Nakauth MACHADO, Luiz Fernando Almeida ISHAK, Marluísa de Oliveira Guimarães ISHAK, Ricardo VALLINOTO, Antonio Carlos Rosário Polimofismo nos genes Lectinas de ligação a manose Proteínas Infecções por Chlamydia The presence of the single nucleotide polymorphisms in exon 1 of the mannose-binding lectin 2 (MBL2) gene was evaluated in a sample of 159 patients undergoing coronary artery bypass surgery (71 patients undergoing valve replacement surgery and 300 control subjects) to investigate a possible association between polymorphisms and heart disease with Chlamydia infection. The identification of the alleles B and D was performed using real time polymerase chain reaction (PCR) and of the allele C was accomplished through PCR assays followed by digestion with the restriction enzyme. The comparative analysis of allelic and genotypic frequencies between the three groups did not reveal any significant difference, even when related to previous Chlamydia infection. Variations in the MBL plasma levels were influenced by the presence of polymorphisms, being significantly higher in the group of cardiac patients, but without representing a risk for the disease. The results showed that despite MBL2 gene polymorphisms being associated with the protein plasma levels, the polymorphisms were not enough to predict the development of heart disease, regardless of infection with both species of Chlamydia. 2017-05-12T14:25:50Z 2017-05-12T14:25:50Z 2016-12 Artigo de Periódico QUEIROZ, Maria Alice Freitas et al. Mannose-binding lectin 2 (Mbl2) gene polymorphisms are related to protein plasma levels, but not to heart disease and infection by Chlamydia. Brazilian Journal of Medical and Biological Research, Ribeirão Preto, v. 49, n. 12, e5519, 2016. Disponível em:<http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016001200604&lng=pt&nrm=iso>. Acesso em: 12 maio 2017. Epub 12-Dez-2016. <http://dx.doi.org/10.1590/1414-431x20165519>. 1414-431X http://repositorio.ufpa.br/jspui/handle/2011/8375 por Brazilian Journal of Medical and Biological Research Acesso Aberto application/pdf Universidade Federal do Pará Brasil UFPA |
| institution |
Repositório Institucional - Universidade Federal do Pará |
| collection |
RI-UFPA |
| language |
por |
| topic |
Polimofismo nos genes Lectinas de ligação a manose Proteínas Infecções por Chlamydia |
| spellingShingle |
Polimofismo nos genes Lectinas de ligação a manose Proteínas Infecções por Chlamydia QUEIROZ, Maria Alice Freitas Mannose-binding lectin 2 (Mbl2) gene polymorphisms are related to protein plasma levels, but not to heart disease and infection by Chlamydia |
| topic_facet |
Polimofismo nos genes Lectinas de ligação a manose Proteínas Infecções por Chlamydia |
| description |
The presence of the single nucleotide polymorphisms in exon 1 of the mannose-binding lectin 2 (MBL2) gene was evaluated in a sample of 159 patients undergoing coronary artery bypass surgery (71 patients undergoing valve replacement surgery and 300 control subjects) to investigate a possible association between polymorphisms and heart disease with Chlamydia infection.
The identification of the alleles B and D was performed using real time polymerase chain reaction (PCR) and of the allele C was accomplished through PCR assays followed by digestion with the restriction enzyme. The comparative analysis of allelic and genotypic frequencies between the three groups did not reveal any significant difference, even when related to previous Chlamydia infection. Variations in the MBL plasma levels were influenced by the presence of polymorphisms, being significantly higher in the group of cardiac patients, but without representing a risk for the disease. The results showed that despite MBL2 gene polymorphisms being associated with the protein plasma levels, the polymorphisms were not enough to predict the development of heart disease, regardless of infection with both species of Chlamydia. |
| format |
Artigo |
| author |
QUEIROZ, Maria Alice Freitas |
| author2 |
GOMES, Samara Tatielle Monteiro ALMEIDA, Núbia Caroline Costa de SOUSA, Maria Izete Machado de COSTA, Suzanne Roberta Cardoso Fernandes HERMES, Renata Bezerra LIMA, Sandra Souza ZANINOTTO, Marcelo Martins FOSSA, Marco Antonio Ayin MANESCYH, Manoel Araujo FEITOSA, Rosimar Neris Martins AZEVEDO, Vânia Nakauth MACHADO, Luiz Fernando Almeida ISHAK, Marluísa de Oliveira Guimarães ISHAK, Ricardo VALLINOTO, Antonio Carlos Rosário |
| author2Str |
GOMES, Samara Tatielle Monteiro ALMEIDA, Núbia Caroline Costa de SOUSA, Maria Izete Machado de COSTA, Suzanne Roberta Cardoso Fernandes HERMES, Renata Bezerra LIMA, Sandra Souza ZANINOTTO, Marcelo Martins FOSSA, Marco Antonio Ayin MANESCYH, Manoel Araujo FEITOSA, Rosimar Neris Martins AZEVEDO, Vânia Nakauth MACHADO, Luiz Fernando Almeida ISHAK, Marluísa de Oliveira Guimarães ISHAK, Ricardo VALLINOTO, Antonio Carlos Rosário |
| title |
Mannose-binding lectin 2 (Mbl2) gene polymorphisms are related to protein plasma levels, but not to heart disease and infection by Chlamydia |
| title_short |
Mannose-binding lectin 2 (Mbl2) gene polymorphisms are related to protein plasma levels, but not to heart disease and infection by Chlamydia |
| title_full |
Mannose-binding lectin 2 (Mbl2) gene polymorphisms are related to protein plasma levels, but not to heart disease and infection by Chlamydia |
| title_fullStr |
Mannose-binding lectin 2 (Mbl2) gene polymorphisms are related to protein plasma levels, but not to heart disease and infection by Chlamydia |
| title_full_unstemmed |
Mannose-binding lectin 2 (Mbl2) gene polymorphisms are related to protein plasma levels, but not to heart disease and infection by Chlamydia |
| title_sort |
mannose-binding lectin 2 (mbl2) gene polymorphisms are related to protein plasma levels, but not to heart disease and infection by chlamydia |
| publisher |
Universidade Federal do Pará |
| publishDate |
2017 |
| url |
http://repositorio.ufpa.br/jspui/handle/2011/8375 |
| _version_ |
1787148265966272512 |
| score |
11.674684 |