Methylmercury inhibits prolactin release in a cell line of pituitary origin

MAUÉS, Luis Antônio Loureiro; MACCHI, Barbarella de Matos; CRESPO LÓPEZ, Maria Elena; NASCIUTTI, Luís Eurico; DINIZ, Domingos Luiz Wanderley Picanço

Artigo

Methylmercury inhibits prolactin release in a cell line of pituitary origin

Heavy metals, such as methylmercury, are key environmental pollutants that easily reach human beings by bioaccumulation through the food chain. Several reports have demonstrated that endocrine organs, and especially the pituitary gland, are potential targets for mercury accumulation; however, the ef...

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Autor principal:	MAUÉS, Luis Antônio Loureiro
Outros Autores:	MACCHI, Barbarella de Matos, CRESPO LÓPEZ, Maria Elena, NASCIUTTI, Luís Eurico, DINIZ, Domingos Luiz Wanderley Picanço
Grau:	Artigo
Idioma:	por
Publicado em:	Universidade Federal do Pará 2017
Assuntos:	Metilmercúrio Prolactina Metais pesados Células secretoras Pituitária Mercúrio
Acesso em linha:	http://repositorio.ufpa.br/jspui/handle/2011/8442 http://dx.doi.org/10.1590/1414-431X20154165

id	ir-2011-8442
recordtype	dspace
spelling	ir-2011-84422018-05-16T17:44:28Z Methylmercury inhibits prolactin release in a cell line of pituitary origin MAUÉS, Luis Antônio Loureiro MACCHI, Barbarella de Matos CRESPO LÓPEZ, Maria Elena NASCIUTTI, Luís Eurico DINIZ, Domingos Luiz Wanderley Picanço Metilmercúrio Prolactina Metais pesados Células secretoras Pituitária Mercúrio Heavy metals, such as methylmercury, are key environmental pollutants that easily reach human beings by bioaccumulation through the food chain. Several reports have demonstrated that endocrine organs, and especially the pituitary gland, are potential targets for mercury accumulation; however, the effects on the regulation of hormonal release are unclear. It has been suggested that serum prolactin could represent a biomarker of heavy metal exposure. The aim of this study was to evaluate the effect of methylmercury on prolactin release and the role of the nitrergic system using prolactin secretory cells (the mammosomatotroph cell line, GH3B6). Exposure to methylmercury (0-100 μM) was cytotoxic in a time- and concentration-dependent manner, with an LC50 higher than described for cells of neuronal origin, suggesting GH3B6 cells have a relative resistance. Methylmercury (at exposures as low as 1 μM for 2 h) also decreased prolactin release. Interestingly, inhibition of nitric oxide synthase by N-nitro-L-arginine completely prevented the decrease in prolactin release without acute neurotoxic effects of methylmercury. These data indicate that the decrease in prolactin production occurs via activation of the nitrergic system and is an early effect of methylmercury in cells of pituitary origin. MAUÉS, L. A. L. Dr. Docente da Universidade Federal do Pará, Campus Universitário de Altamira MACCHI, B. de M. Dr. Docente da Universidade Federal do Pará, Instituto de Ciências Biológicas CRESPO LÓPEZ, M. E. Dr. Docente da Universidade Federal do Pará, Insituto de Ciências Biológicas 2017-05-17T16:37:47Z 2017-05-17T16:37:47Z 2015-08 Artigo de Periódico MAUES, Luís Antônio Loureiro et al. Methylmercury inhibits prolactin release in a cell line of pituitary origin. Brazilian Journal of Medical and Biological Research, Ribeirão Preto, v. 48, n. 8, p. 691-696, ago. 2015. Disponível em: <http://repositorio.ufpa.br/jspui/handle/2011/8442>. Acesso em:. 1414-431X http://repositorio.ufpa.br/jspui/handle/2011/8442 http://dx.doi.org/10.1590/1414-431X20154165 por Brazilian Journal of Medical and Biological Research Acesso Aberto application/pdf Universidade Federal do Pará Brasil UFPA http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000800691&lng=pt&nrm=iso
institution	Repositório Institucional - Universidade Federal do Pará
collection	RI-UFPA
language	por
topic	Metilmercúrio Prolactina Metais pesados Células secretoras Pituitária Mercúrio
spellingShingle	Metilmercúrio Prolactina Metais pesados Células secretoras Pituitária Mercúrio MAUÉS, Luis Antônio Loureiro Methylmercury inhibits prolactin release in a cell line of pituitary origin
topic_facet	Metilmercúrio Prolactina Metais pesados Células secretoras Pituitária Mercúrio
description	Heavy metals, such as methylmercury, are key environmental pollutants that easily reach human beings by bioaccumulation through the food chain. Several reports have demonstrated that endocrine organs, and especially the pituitary gland, are potential targets for mercury accumulation; however, the effects on the regulation of hormonal release are unclear. It has been suggested that serum prolactin could represent a biomarker of heavy metal exposure. The aim of this study was to evaluate the effect of methylmercury on prolactin release and the role of the nitrergic system using prolactin secretory cells (the mammosomatotroph cell line, GH3B6). Exposure to methylmercury (0-100 μM) was cytotoxic in a time- and concentration-dependent manner, with an LC50 higher than described for cells of neuronal origin, suggesting GH3B6 cells have a relative resistance. Methylmercury (at exposures as low as 1 μM for 2 h) also decreased prolactin release. Interestingly, inhibition of nitric oxide synthase by N-nitro-L-arginine completely prevented the decrease in prolactin release without acute neurotoxic effects of methylmercury. These data indicate that the decrease in prolactin production occurs via activation of the nitrergic system and is an early effect of methylmercury in cells of pituitary origin.
format	Artigo
author	MAUÉS, Luis Antônio Loureiro
author2	MACCHI, Barbarella de Matos CRESPO LÓPEZ, Maria Elena NASCIUTTI, Luís Eurico DINIZ, Domingos Luiz Wanderley Picanço
author2Str	MACCHI, Barbarella de Matos CRESPO LÓPEZ, Maria Elena NASCIUTTI, Luís Eurico DINIZ, Domingos Luiz Wanderley Picanço
title	Methylmercury inhibits prolactin release in a cell line of pituitary origin
title_short	Methylmercury inhibits prolactin release in a cell line of pituitary origin
title_full	Methylmercury inhibits prolactin release in a cell line of pituitary origin
title_fullStr	Methylmercury inhibits prolactin release in a cell line of pituitary origin
title_full_unstemmed	Methylmercury inhibits prolactin release in a cell line of pituitary origin
title_sort	methylmercury inhibits prolactin release in a cell line of pituitary origin
publisher	Universidade Federal do Pará
publishDate	2017
url	http://repositorio.ufpa.br/jspui/handle/2011/8442 http://dx.doi.org/10.1590/1414-431X20154165
_version_	1832602264852758528
score	11.755432

Methylmercury inhibits prolactin release in a cell line of pituitary origin

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