This study investigated the occurrence of Leptospira spp. in buffaloes in the Amazon biome of Pará, as well as identify the higher incidence of serotypes in the region. They tested 387 serum samples of buffalo nine Pará municipalities, Cachoeira do Arari, Santa Cruz do Arari, Salvaterra, Soure and Chaves located on Marajó Island, Abaetetuba, Ipixuna, Nova Timboteua and Paragominas, located on the mainland. Eight serotypes were tested: Bataviae, Bratislava, Hardjobovis, Hardjo (OMS), Hardjo (CTG), Icterohaemorrhagiae, Pomona and Hardjo (Bolivia), through the microscopic agglutination test method (SAM). Of the total samples tested 354 (91.50%) were reactive serum for at least one serovar and 33 (8.50%) were negative. Three serotypes of serogroup Sejroe were more frequent, Hardjo (Bolivia) (79.30%), Hardjo (OMS) (64.8%) and Hardjobovis (64.10%). It is important to know the higher incidence of serotypes in the region studied so that the directions of combat and infection control are efficient, minimizing the potential risk of contamination and maintenance of leptospirosis in the herd.
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