The development of an enzyme-linked immunosorbent assay for Trypanosoma vivax antibodies and its use in epidemiological surveys

MADRUGA, Cláudio Roberto; ARAÚJO, Flábio Ribeiro de; CAVALCANTE, Gustavo Góes; MARTINS, Charles Ferreira; BARBOSA, Imke Barbara Pfeifer; RIBEIRO, Laura Rios; KESSLER, Raul Henrique; SOARES, Cleber Oliveira; MIGUITA, Midori; MELO, Elaine Silva de Pádua; ALMEIDA, Robson Ferreira Cavalcante de; LIMA JUNIOR, Manoel Sebastião da Costa

Artigo

The development of an enzyme-linked immunosorbent assay for Trypanosoma vivax antibodies and its use in epidemiological surveys

There are data indicating that the distribution of Trypanosoma vivax in the Brazilian territory is expanding with potential to reach other areas, where the vectors are present. The detection of anti-trypanosomal antibodies in serum provides important information of the trypanosomal status in cattle...

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Autor principal:	MADRUGA, Cláudio Roberto
Outros Autores:	ARAÚJO, Flábio Ribeiro de, CAVALCANTE, Gustavo Góes, MARTINS, Charles Ferreira, BARBOSA, Imke Barbara Pfeifer, RIBEIRO, Laura Rios, KESSLER, Raul Henrique, SOARES, Cleber Oliveira, MIGUITA, Midori, MELO, Elaine Silva de Pádua, ALMEIDA, Robson Ferreira Cavalcante de, LIMA JUNIOR, Manoel Sebastião da Costa
Grau:	Artigo
Idioma:	por
Publicado em:	Universidade Federal do Pará 2017
Assuntos:	Enzimas Trypanosoma vivax Pesquisas epidemiológicas Anticorpos Epistemologia
Acesso em linha:	http://repositorio.ufpa.br/jspui/handle/2011/8889

id	ir-2011-8889
recordtype	dspace
spelling	ir-2011-88892017-10-16T12:27:24Z The development of an enzyme-linked immunosorbent assay for Trypanosoma vivax antibodies and its use in epidemiological surveys MADRUGA, Cláudio Roberto ARAÚJO, Flábio Ribeiro de CAVALCANTE, Gustavo Góes MARTINS, Charles Ferreira BARBOSA, Imke Barbara Pfeifer RIBEIRO, Laura Rios KESSLER, Raul Henrique SOARES, Cleber Oliveira MIGUITA, Midori MELO, Elaine Silva de Pádua ALMEIDA, Robson Ferreira Cavalcante de LIMA JUNIOR, Manoel Sebastião da Costa Enzimas Trypanosoma vivax Pesquisas epidemiológicas Anticorpos Epistemologia There are data indicating that the distribution of Trypanosoma vivax in the Brazilian territory is expanding with potential to reach other areas, where the vectors are present. The detection of anti-trypanosomal antibodies in serum provides important information of the trypanosomal status in cattle herds. For this reason, an enzyme-linked immunosorbent assay (Tv-ELISA-Ab) with crude antigen from one Brazilian isolate of T. vivax was developed and evaluated. The sensitivity and specificity were respectively 97.6 and 96.9%. In the evaluation of cross-reactions, three calves inoculated with T. evansi trypimastigotes blood forms showed optical densities (OD) under the cut-off during the whole experimental period, except one at 45 days post-inoculation. With relation to Babesia bovis, B. bigemina, and Anaplasma marginale, which are endemic hemoparasites in the studied area, the cross-reactions were shown to be 5.7, 5.3, and 1.1%, respectively. The first serological survey of Pantanal and state of Pará showed that T. vivax is widespread, although regions within both areas had significantly different prevalences. Therefore, this Tv-ELISA-Ab may be a more appropriate test for epidemiological studies in developing countries because the diagnostic laboratories in most countries may be able to perform an ELISA, which is not true for polymerase chain reaction. 2017-07-21T14:00:32Z 2017-07-21T14:00:32Z 2006-11 Artigo de Periódico MADRUGA, Cláudio Roberto et al. The development of an enzyme-linked immunosorbent assay for Trypanosoma vivax antibodies and its use in epidemiological surveys. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 101, n. 7, p. 801-807, nov. 2006. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762006000700016&lng=pt&nrm=iso>. Acesso em: 21 jul. 2017. <http://dx.doi.org/10.1590/S0074-02762006000700016>. 1678-8060 http://repositorio.ufpa.br/jspui/handle/2011/8889 por Memórias do Instituto Oswaldo Cruz Acesso Aberto application/pdf Universidade Federal do Pará Brasil UFPA
institution	Repositório Institucional - Universidade Federal do Pará
collection	RI-UFPA
language	por
topic	Enzimas Trypanosoma vivax Pesquisas epidemiológicas Anticorpos Epistemologia
spellingShingle	Enzimas Trypanosoma vivax Pesquisas epidemiológicas Anticorpos Epistemologia MADRUGA, Cláudio Roberto The development of an enzyme-linked immunosorbent assay for Trypanosoma vivax antibodies and its use in epidemiological surveys
topic_facet	Enzimas Trypanosoma vivax Pesquisas epidemiológicas Anticorpos Epistemologia
description	There are data indicating that the distribution of Trypanosoma vivax in the Brazilian territory is expanding with potential to reach other areas, where the vectors are present. The detection of anti-trypanosomal antibodies in serum provides important information of the trypanosomal status in cattle herds. For this reason, an enzyme-linked immunosorbent assay (Tv-ELISA-Ab) with crude antigen from one Brazilian isolate of T. vivax was developed and evaluated. The sensitivity and specificity were respectively 97.6 and 96.9%. In the evaluation of cross-reactions, three calves inoculated with T. evansi trypimastigotes blood forms showed optical densities (OD) under the cut-off during the whole experimental period, except one at 45 days post-inoculation. With relation to Babesia bovis, B. bigemina, and Anaplasma marginale, which are endemic hemoparasites in the studied area, the cross-reactions were shown to be 5.7, 5.3, and 1.1%, respectively. The first serological survey of Pantanal and state of Pará showed that T. vivax is widespread, although regions within both areas had significantly different prevalences. Therefore, this Tv-ELISA-Ab may be a more appropriate test for epidemiological studies in developing countries because the diagnostic laboratories in most countries may be able to perform an ELISA, which is not true for polymerase chain reaction.
format	Artigo
author	MADRUGA, Cláudio Roberto
author2	ARAÚJO, Flábio Ribeiro de CAVALCANTE, Gustavo Góes MARTINS, Charles Ferreira BARBOSA, Imke Barbara Pfeifer RIBEIRO, Laura Rios KESSLER, Raul Henrique SOARES, Cleber Oliveira MIGUITA, Midori MELO, Elaine Silva de Pádua ALMEIDA, Robson Ferreira Cavalcante de LIMA JUNIOR, Manoel Sebastião da Costa
author2Str	ARAÚJO, Flábio Ribeiro de CAVALCANTE, Gustavo Góes MARTINS, Charles Ferreira BARBOSA, Imke Barbara Pfeifer RIBEIRO, Laura Rios KESSLER, Raul Henrique SOARES, Cleber Oliveira MIGUITA, Midori MELO, Elaine Silva de Pádua ALMEIDA, Robson Ferreira Cavalcante de LIMA JUNIOR, Manoel Sebastião da Costa
title	The development of an enzyme-linked immunosorbent assay for Trypanosoma vivax antibodies and its use in epidemiological surveys
title_short	The development of an enzyme-linked immunosorbent assay for Trypanosoma vivax antibodies and its use in epidemiological surveys
title_full	The development of an enzyme-linked immunosorbent assay for Trypanosoma vivax antibodies and its use in epidemiological surveys
title_fullStr	The development of an enzyme-linked immunosorbent assay for Trypanosoma vivax antibodies and its use in epidemiological surveys
title_full_unstemmed	The development of an enzyme-linked immunosorbent assay for Trypanosoma vivax antibodies and its use in epidemiological surveys
title_sort	development of an enzyme-linked immunosorbent assay for trypanosoma vivax antibodies and its use in epidemiological surveys
publisher	Universidade Federal do Pará
publishDate	2017
url	http://repositorio.ufpa.br/jspui/handle/2011/8889
_version_	1832604281809666048
score	11.755432

The development of an enzyme-linked immunosorbent assay for Trypanosoma vivax antibodies and its use in epidemiological surveys

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