Artigo

Detecção de Brucella abortus em linfonodos de búfalas (Bubalus bubalis) em diferentes fases da gestação

The objective of this study was to detect Brucella abortus in lymph nodes of buffaloes as well as to describe the lesions caused. Nineteen buffalo cows in various stages of pregnancy, serologically positive for brucellosis and subjected to culling were used. Fetal age was determined by ultrasound ex...

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Autor principal: SOUSA, Melina Garcia Saraiva de
Outros Autores: BRITO, Marilene de Farias, UBIALI, Daniel Guimarães, FONSECA JÚNIOR, Antônio Augusto, SILVA, Jenevaldo Barbosa da, REIS, Alessandra dos Santos Belo, OLIVEIRA, Carlos Magno Chaves, BARBOSA NETO, José Diomedes
Grau: Artigo
Idioma: por
Publicado em: Universidade Federal do Pará 2017
Assuntos:
DNA
Acesso em linha: http://repositorio.ufpa.br/jspui/handle/2011/8946
Resumo:
The objective of this study was to detect Brucella abortus in lymph nodes of buffaloes as well as to describe the lesions caused. Nineteen buffalo cows in various stages of pregnancy, serologically positive for brucellosis and subjected to culling were used. Fetal age was determined by ultrasound examination and the size of fetuses was measured at necropsy. Fragments of lymph nodes were collected for histopathology and qPCR. The detection of B. abortus DNA in the lymph nodes was checked from the fourth month of pregnancy in seven buffaloes and in a post-calving buffalo. Acute to chronic lymphadenitis was histologically diagnosed. B. abortus DNA was detected in all evaluated groups of lymph nodes; the mammary lymph nodes were the most affected.