Bovine lactoferrin activity against Chikungunya and Zika viruses.
Chikungunya (CHIKV) and Zika (ZIKV) viruses are arboviruses which have recently broken their sylvatic isolation and gone on to spread rampantly among humans in some urban areas of the world, especially in Latin America. Given the lack of effective interventions against such viruses, the aim of this...
| Autor principal: | Carvalho, Carlos A. M. |
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| Outros Autores: | Casseb, Samir Mansour M., Gon?alves, Rafael B., Silva, Eliana Vieira Pinto da, Gomes, Andre M. O., Vasconcelos, Pedro Fernando da Costa |
| Grau: | Artigo |
| Idioma: | eng |
| Publicado em: |
Microbiology Society
2017
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| Acesso em linha: |
http://patua.iec.gov.br//handle/iec/2752 |
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ir-iec-2752 |
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ir-iec-27522019-04-26T18:18:06Z Bovine lactoferrin activity against Chikungunya and Zika viruses. Carvalho, Carlos A. M. Casseb, Samir Mansour M. Gon?alves, Rafael B. Silva, Eliana Vieira Pinto da Gomes, Andre M. O. Vasconcelos, Pedro Fernando da Costa Arbovirus Antivirais / uso terap?utico V?rus Chikungunya / efeitos de drogas Zika virus / efeitos de drogas Zika virus / efeitos de drogas Lactoferrina / administra??o & dosagem Lactoferrina / antagonistas & inibidores Lactoferrina / efeitos adversos Lactoferrina / uso terap?utico Resultado do Tratamento Citotoxicidade Imunol?gica RNA Viral / isolamento & purifica??o C?lulas Vero Rea??o em Cadeia da Polimerase Via Transcriptase Reversa / m?todos T?cnica Indireta de Fluoresc?ncia para Anticorpo / m?todos Sobreviv?ncia Celular / efeitos de drogas Chikungunya (CHIKV) and Zika (ZIKV) viruses are arboviruses which have recently broken their sylvatic isolation and gone on to spread rampantly among humans in some urban areas of the world, especially in Latin America. Given the lack of effective interventions against such viruses, the aim of this work was to evaluate the antiviral potential of bovine lactoferrin (bLf) in their infections. Through viability, plaque, immunofluorescence and nucleic acid quantification assays, our data show that bLf exerts a dose-dependent strong inhibitory effect on the infection of Vero cells by the aforementioned arboviruses, reducing their infection efficiency by up to nearly 80 %, with no expressive cytotoxicity, and that such antiviral activity occurs at the levels of input and output of virus particles. These findings reveal that bLf antimicrobial properties are extendable to CHIKV and ZIKV, underlining a generic inhibition mechanism that can be explored to develop a potential strategy against their infections. 2017-09-15T08:35:20Z 2017-09-15T08:35:20Z 2017 Artigo CARVALHO, Carlos A. M. et al. Bovine lactoferrin activity against Chikungunya and Zika viruses. Journal of General Virology, v. 98, n. 7, p. 1749-1754, July 2017. 1465-2099 http://patua.iec.gov.br//handle/iec/2752 10.1099/jgv.0.000849 eng Acesso Aberto application/pdf Microbiology Society |
| institution |
Instituto Evandro Chagas (IEC) |
| collection |
PATUA |
| language |
eng |
| topic |
Arbovirus Antivirais / uso terap?utico V?rus Chikungunya / efeitos de drogas Zika virus / efeitos de drogas Zika virus / efeitos de drogas Lactoferrina / administra??o & dosagem Lactoferrina / antagonistas & inibidores Lactoferrina / efeitos adversos Lactoferrina / uso terap?utico Resultado do Tratamento Citotoxicidade Imunol?gica RNA Viral / isolamento & purifica??o C?lulas Vero Rea??o em Cadeia da Polimerase Via Transcriptase Reversa / m?todos T?cnica Indireta de Fluoresc?ncia para Anticorpo / m?todos Sobreviv?ncia Celular / efeitos de drogas |
| spellingShingle |
Arbovirus Antivirais / uso terap?utico V?rus Chikungunya / efeitos de drogas Zika virus / efeitos de drogas Zika virus / efeitos de drogas Lactoferrina / administra??o & dosagem Lactoferrina / antagonistas & inibidores Lactoferrina / efeitos adversos Lactoferrina / uso terap?utico Resultado do Tratamento Citotoxicidade Imunol?gica RNA Viral / isolamento & purifica??o C?lulas Vero Rea??o em Cadeia da Polimerase Via Transcriptase Reversa / m?todos T?cnica Indireta de Fluoresc?ncia para Anticorpo / m?todos Sobreviv?ncia Celular / efeitos de drogas Carvalho, Carlos A. M. Bovine lactoferrin activity against Chikungunya and Zika viruses. |
| topic_facet |
Arbovirus Antivirais / uso terap?utico V?rus Chikungunya / efeitos de drogas Zika virus / efeitos de drogas Zika virus / efeitos de drogas Lactoferrina / administra??o & dosagem Lactoferrina / antagonistas & inibidores Lactoferrina / efeitos adversos Lactoferrina / uso terap?utico Resultado do Tratamento Citotoxicidade Imunol?gica RNA Viral / isolamento & purifica??o C?lulas Vero Rea??o em Cadeia da Polimerase Via Transcriptase Reversa / m?todos T?cnica Indireta de Fluoresc?ncia para Anticorpo / m?todos Sobreviv?ncia Celular / efeitos de drogas |
| description |
Chikungunya (CHIKV) and Zika (ZIKV) viruses are arboviruses which have recently broken their sylvatic isolation and gone on to spread rampantly among humans in some urban areas of the world, especially in Latin America. Given the lack of effective interventions against such viruses, the aim of this work was to evaluate the antiviral potential of bovine lactoferrin (bLf) in their infections. Through viability, plaque, immunofluorescence and nucleic acid quantification assays, our data show that bLf exerts a dose-dependent strong inhibitory effect on the infection of Vero cells by the aforementioned arboviruses, reducing their infection efficiency by up to nearly 80 %, with no expressive cytotoxicity, and that such antiviral activity occurs at the levels of input and output of virus particles. These findings reveal that bLf antimicrobial properties are extendable to CHIKV and ZIKV, underlining a generic inhibition mechanism that can be explored to develop a potential strategy against their infections. |
| format |
Artigo |
| author |
Carvalho, Carlos A. M. |
| author2 |
Casseb, Samir Mansour M. Gon?alves, Rafael B. Silva, Eliana Vieira Pinto da Gomes, Andre M. O. Vasconcelos, Pedro Fernando da Costa |
| author2Str |
Casseb, Samir Mansour M. Gon?alves, Rafael B. Silva, Eliana Vieira Pinto da Gomes, Andre M. O. Vasconcelos, Pedro Fernando da Costa |
| title |
Bovine lactoferrin activity against Chikungunya and Zika viruses. |
| title_short |
Bovine lactoferrin activity against Chikungunya and Zika viruses. |
| title_full |
Bovine lactoferrin activity against Chikungunya and Zika viruses. |
| title_fullStr |
Bovine lactoferrin activity against Chikungunya and Zika viruses. |
| title_full_unstemmed |
Bovine lactoferrin activity against Chikungunya and Zika viruses. |
| title_sort |
bovine lactoferrin activity against chikungunya and zika viruses. |
| publisher |
Microbiology Society |
| publishDate |
2017 |
| url |
http://patua.iec.gov.br//handle/iec/2752 |
| _version_ |
1717584530288672768 |
| score |
11.755432 |