Analysis of a reverse transcription loop-mediated isothermal amplification (RT-LAMP) for yellow fever diagnostic

Nunes, Marcio Roberto Teixeira; Vianez J?nior, Jo?o L?dio da Silva Gon?alves; Nunes, Keley Nascimento Barbosa; Silva, Sandro Patroca da; Lima, Clayton Pereira Silva de; Guzman, Hilda; Martins, L?via Caricio; Carvalho, Val?ria Lima; Tesh, Robert B; Vasconcelos, Pedro Fernando da Costa

Artigo

Analysis of a reverse transcription loop-mediated isothermal amplification (RT-LAMP) for yellow fever diagnostic

Yellow Fever virus (YFV) is an important human pathogen in tropical areas of Africa and South America. Although an efficient vaccine is available and has been used since the early 1940s, sylvatic YFV transmission still occurs in forested areas where anthropogenic actions are present, such as mineral...

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Autor principal:	Nunes, Marcio Roberto Teixeira
Outros Autores:	Vianez J?nior, Jo?o L?dio da Silva Gon?alves, Nunes, Keley Nascimento Barbosa, Silva, Sandro Patroca da, Lima, Clayton Pereira Silva de, Guzman, Hilda, Martins, L?via Caricio, Carvalho, Val?ria Lima, Tesh, Robert B, Vasconcelos, Pedro Fernando da Costa
Grau:	Artigo
Idioma:	eng
Publicado em:	Elsevier 2017
Assuntos:	V?rus da Febre Amarela / isolamento & purifica??o Febre Amarela / diagn?stico Febre Amarela / virologia Transcri??o Reversa Rea??o em Cadeia da Polimerase Via Transcriptase Reversa / m?todos T?cnicas de Amplifica??o de ?cido Nucleico / m?todos
Acesso em linha:	http://patua.iec.gov.br//handle/iec/2760

id	ir-iec-2760
recordtype	dspace
spelling	ir-iec-27602019-05-31T19:18:13Z Analysis of a reverse transcription loop-mediated isothermal amplification (RT-LAMP) for yellow fever diagnostic Nunes, Marcio Roberto Teixeira Vianez J?nior, Jo?o L?dio da Silva Gon?alves Nunes, Keley Nascimento Barbosa Silva, Sandro Patroca da Lima, Clayton Pereira Silva de Guzman, Hilda Martins, L?via Caricio Carvalho, Val?ria Lima Tesh, Robert B Vasconcelos, Pedro Fernando da Costa V?rus da Febre Amarela / isolamento & purifica??o Febre Amarela / diagn?stico Febre Amarela / virologia Transcri??o Reversa Rea??o em Cadeia da Polimerase Via Transcriptase Reversa / m?todos T?cnicas de Amplifica??o de ?cido Nucleico / m?todos Yellow Fever virus (YFV) is an important human pathogen in tropical areas of Africa and South America. Although an efficient vaccine is available and has been used since the early 1940s, sylvatic YFV transmission still occurs in forested areas where anthropogenic actions are present, such as mineral extraction, rearing livestock and agriculture, and ecological tourism. In this context, two distinct techniques based on the RT-PCR derived method have been previously developed, however both methods are expensive due to the use of thermo cyclers and labeled probes. We developed isothermal genome amplification, which is a rapid, sensitive, specific and low cost molecular approach for YFV genome detection. This assay used a set of degenerate primers designed for the NS1 gene and was able to amplify, within 30 min in isothermal conditions, the YFV 17D vaccine strain derived from an African wild prototype strain (Asibi),as well as field strains from Brazil, other endemic countries from South and Central America, and the Caribbean. The generic RT-LAMP assay could be helpful for YFV surveillance in field and rapid response during outbreaks in endemic areas. This study was partially supported by CNPq (Conselho Nacional para o Desenvolvimento Cient?fico e Tecnol?gico) projects 401558/2013-4; 457664/2013-4, 486069/2012-5 and 301641/2010-2), and CNPq/CAPES/FAPESPA (project 573739-2008-0). 2017-09-22T09:47:38Z 2017-09-22T09:47:38Z 2015 Artigo NUNES, Marcio Roberto Teixeira et al. Analysis of a reverse transcription loop-mediated isothermal amplification (RT-LAMP) for yellow fever diagnostic. Journal of Virological Methods, v. 226, p. 40-51, Dec. 2015. 1879-0984 http://patua.iec.gov.br//handle/iec/2760 10.1016/j.jviromet.2015.10.003 eng Acesso Aberto application/pdf Elsevier
institution	Instituto Evandro Chagas (IEC)
collection	PATUA
language	eng
topic	V?rus da Febre Amarela / isolamento & purifica??o Febre Amarela / diagn?stico Febre Amarela / virologia Transcri??o Reversa Rea??o em Cadeia da Polimerase Via Transcriptase Reversa / m?todos T?cnicas de Amplifica??o de ?cido Nucleico / m?todos
spellingShingle	V?rus da Febre Amarela / isolamento & purifica??o Febre Amarela / diagn?stico Febre Amarela / virologia Transcri??o Reversa Rea??o em Cadeia da Polimerase Via Transcriptase Reversa / m?todos T?cnicas de Amplifica??o de ?cido Nucleico / m?todos Nunes, Marcio Roberto Teixeira Analysis of a reverse transcription loop-mediated isothermal amplification (RT-LAMP) for yellow fever diagnostic
topic_facet	V?rus da Febre Amarela / isolamento & purifica??o Febre Amarela / diagn?stico Febre Amarela / virologia Transcri??o Reversa Rea??o em Cadeia da Polimerase Via Transcriptase Reversa / m?todos T?cnicas de Amplifica??o de ?cido Nucleico / m?todos
description	Yellow Fever virus (YFV) is an important human pathogen in tropical areas of Africa and South America. Although an efficient vaccine is available and has been used since the early 1940s, sylvatic YFV transmission still occurs in forested areas where anthropogenic actions are present, such as mineral extraction, rearing livestock and agriculture, and ecological tourism. In this context, two distinct techniques based on the RT-PCR derived method have been previously developed, however both methods are expensive due to the use of thermo cyclers and labeled probes. We developed isothermal genome amplification, which is a rapid, sensitive, specific and low cost molecular approach for YFV genome detection. This assay used a set of degenerate primers designed for the NS1 gene and was able to amplify, within 30 min in isothermal conditions, the YFV 17D vaccine strain derived from an African wild prototype strain (Asibi),as well as field strains from Brazil, other endemic countries from South and Central America, and the Caribbean. The generic RT-LAMP assay could be helpful for YFV surveillance in field and rapid response during outbreaks in endemic areas.
format	Artigo
author	Nunes, Marcio Roberto Teixeira
author2	Vianez J?nior, Jo?o L?dio da Silva Gon?alves Nunes, Keley Nascimento Barbosa Silva, Sandro Patroca da Lima, Clayton Pereira Silva de Guzman, Hilda Martins, L?via Caricio Carvalho, Val?ria Lima Tesh, Robert B Vasconcelos, Pedro Fernando da Costa
author2Str	Vianez J?nior, Jo?o L?dio da Silva Gon?alves Nunes, Keley Nascimento Barbosa Silva, Sandro Patroca da Lima, Clayton Pereira Silva de Guzman, Hilda Martins, L?via Caricio Carvalho, Val?ria Lima Tesh, Robert B Vasconcelos, Pedro Fernando da Costa
title	Analysis of a reverse transcription loop-mediated isothermal amplification (RT-LAMP) for yellow fever diagnostic
title_short	Analysis of a reverse transcription loop-mediated isothermal amplification (RT-LAMP) for yellow fever diagnostic
title_full	Analysis of a reverse transcription loop-mediated isothermal amplification (RT-LAMP) for yellow fever diagnostic
title_fullStr	Analysis of a reverse transcription loop-mediated isothermal amplification (RT-LAMP) for yellow fever diagnostic
title_full_unstemmed	Analysis of a reverse transcription loop-mediated isothermal amplification (RT-LAMP) for yellow fever diagnostic
title_sort	analysis of a reverse transcription loop-mediated isothermal amplification (rt-lamp) for yellow fever diagnostic
publisher	Elsevier
publishDate	2017
url	http://patua.iec.gov.br//handle/iec/2760
_version_	1717584443295662080
score	11.653393

Analysis of a reverse transcription loop-mediated isothermal amplification (RT-LAMP) for yellow fever diagnostic

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