Further evidence associating IgG1, but not IgG2, with susceptibility to canine visceral leishmaniasis caused by Leishmania (L.) infantum chagasi-infection
We present here a cross-sectional study analyzing the IgG1 and IgG2 immune responses to natural canine Leishmania (L.) infantum chagasi-infection and their relationships with delayed-type hypersensitivity (DTH) in 50?mongrel dogs with previous positive serodiagnoses (IFAT-IgG) (56% with subclinical...
| Autor principal: | Lima, Luciana Vieira do R. |
|---|---|
| Outros Autores: | Carneiro, Liliane Almeida, Campos, Marliane Batista, Santos, Thiago Vasconcelos dos, Ramos, Patricia Karla, Laurenti, M?rcia Dalastra, Teixeira, Claudio Eduardo C., Silveira, Fernando Tobias |
| Grau: | Artigo |
| Idioma: | eng |
| Publicado em: |
EDP Open
2017
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| Assuntos: | |
| Acesso em linha: |
http://patua.iec.gov.br//handle/iec/2858 |
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ir-iec-2858 |
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ir-iec-28582019-04-26T17:32:16Z Further evidence associating IgG1, but not IgG2, with susceptibility to canine visceral leishmaniasis caused by Leishmania (L.) infantum chagasi-infection Lima, Luciana Vieira do R. Carneiro, Liliane Almeida Campos, Marliane Batista Santos, Thiago Vasconcelos dos Ramos, Patricia Karla Laurenti, M?rcia Dalastra Teixeira, Claudio Eduardo C. Silveira, Fernando Tobias Leishmania infantum / parasitologia Leishmania infantum / patogenicidade Leishmaniose Visceral / patogenicidade Leishmaniose Visceral / parasitologia C?es / parasitologia Imunoglobulina G / imunologia Ensaio de Imunoadsor??o Enzim?tica / m?todos We present here a cross-sectional study analyzing the IgG1 and IgG2 immune responses to natural canine Leishmania (L.) infantum chagasi-infection and their relationships with delayed-type hypersensitivity (DTH) in 50?mongrel dogs with previous positive serodiagnoses (IFAT-IgG) (56% with subclinical status [=?apparently healthy] and 44% clinically sick), living in endemic areas for visceral leishmaniasis in the Brazilian Amazon. IgG1 and IgG2 responses were measured using commercial polyclonal antibodies in ELISA, while DTH was elicited by intradermal skin test using cultured promastigotes L. (L.) i. chagasi-antigen. Data analyses used Chi-square and Pearson's r coefficient (95% confidence interval). Regarding DTH and the clinical statuses of dogs, it was noted that 100% of the animals showing positive DTH (n?=?8) were from the subclinical group, while 100% showing negative DTH were from the clinically sick group; higher IgG2 than IgG1 responses were observed in both clinical groups. However, when this comparison was made between the subclinical and sick groups, higher IgG1 responses were noted in the dogs from the sick rather than the subclinical group, while no differences were noted between the IgG2 responses in the dogs from both clinical groups. Additionally, we found lower IgG1 responses in dogs from the subclinical group showing positive DTH than in the dogs from the subclinical or sick groups with negative DTH; no differences were found between the IgG2 responses of these two clinical groups. These findings suggest that the IgG1, but not the IgG2, response is associated with susceptibility to canine visceral leishmaniasis (CVL). This research was supported by the Evandro Chagas Institute (Ministry of Health, Brazil) and Nucleus of Tropical Medicine (Federal University of Par? State, Brazil). 2017-11-20T18:25:43Z 2017-11-20T18:25:43Z 2017 Artigo LIMA, Luciana Vieira do R. et al. Further evidence associating IgG1, but not IgG2, with susceptibility to canine visceral leishmaniasis caused by Leishmania (L.) infantum chagasi-infection. Parasite, v. 24, n. 37, p. 1-11, Oct. 2017. 1252-607X http://patua.iec.gov.br//handle/iec/2858 10.1051/parasite/2017039 eng Acesso Aberto application/pdf EDP Open |
| institution |
Instituto Evandro Chagas (IEC) |
| collection |
PATUA |
| language |
eng |
| topic |
Leishmania infantum / parasitologia Leishmania infantum / patogenicidade Leishmaniose Visceral / patogenicidade Leishmaniose Visceral / parasitologia C?es / parasitologia Imunoglobulina G / imunologia Ensaio de Imunoadsor??o Enzim?tica / m?todos |
| spellingShingle |
Leishmania infantum / parasitologia Leishmania infantum / patogenicidade Leishmaniose Visceral / patogenicidade Leishmaniose Visceral / parasitologia C?es / parasitologia Imunoglobulina G / imunologia Ensaio de Imunoadsor??o Enzim?tica / m?todos Lima, Luciana Vieira do R. Further evidence associating IgG1, but not IgG2, with susceptibility to canine visceral leishmaniasis caused by Leishmania (L.) infantum chagasi-infection |
| topic_facet |
Leishmania infantum / parasitologia Leishmania infantum / patogenicidade Leishmaniose Visceral / patogenicidade Leishmaniose Visceral / parasitologia C?es / parasitologia Imunoglobulina G / imunologia Ensaio de Imunoadsor??o Enzim?tica / m?todos |
| description |
We present here a cross-sectional study analyzing the IgG1 and IgG2 immune responses to natural canine Leishmania (L.) infantum chagasi-infection and their relationships with delayed-type hypersensitivity (DTH) in 50?mongrel dogs with previous positive serodiagnoses (IFAT-IgG) (56% with subclinical status [=?apparently healthy] and 44% clinically sick), living in endemic areas for visceral leishmaniasis in the Brazilian Amazon. IgG1 and IgG2 responses were measured using commercial polyclonal antibodies in ELISA, while DTH was elicited by intradermal skin test using cultured promastigotes L. (L.) i. chagasi-antigen. Data analyses used Chi-square and Pearson's r coefficient (95% confidence interval). Regarding DTH and the clinical statuses of dogs, it was noted that 100% of the animals showing positive DTH (n?=?8) were from the subclinical group, while 100% showing negative DTH were from the clinically sick group; higher IgG2 than IgG1 responses were observed in both clinical groups. However, when this comparison was made between the subclinical and sick groups, higher IgG1 responses were noted in the dogs from the sick rather than the subclinical group, while no differences were noted between the IgG2 responses in the dogs from both clinical groups. Additionally, we found lower IgG1 responses in dogs from the subclinical group showing positive DTH than in the dogs from the subclinical or sick groups with negative DTH; no differences were found between the IgG2 responses of these two clinical groups. These findings suggest that the IgG1, but not the IgG2, response is associated with susceptibility to canine visceral leishmaniasis (CVL). |
| format |
Artigo |
| author |
Lima, Luciana Vieira do R. |
| author2 |
Carneiro, Liliane Almeida Campos, Marliane Batista Santos, Thiago Vasconcelos dos Ramos, Patricia Karla Laurenti, M?rcia Dalastra Teixeira, Claudio Eduardo C. Silveira, Fernando Tobias |
| author2Str |
Carneiro, Liliane Almeida Campos, Marliane Batista Santos, Thiago Vasconcelos dos Ramos, Patricia Karla Laurenti, M?rcia Dalastra Teixeira, Claudio Eduardo C. Silveira, Fernando Tobias |
| title |
Further evidence associating IgG1, but not IgG2, with susceptibility to canine visceral leishmaniasis caused by Leishmania (L.) infantum chagasi-infection |
| title_short |
Further evidence associating IgG1, but not IgG2, with susceptibility to canine visceral leishmaniasis caused by Leishmania (L.) infantum chagasi-infection |
| title_full |
Further evidence associating IgG1, but not IgG2, with susceptibility to canine visceral leishmaniasis caused by Leishmania (L.) infantum chagasi-infection |
| title_fullStr |
Further evidence associating IgG1, but not IgG2, with susceptibility to canine visceral leishmaniasis caused by Leishmania (L.) infantum chagasi-infection |
| title_full_unstemmed |
Further evidence associating IgG1, but not IgG2, with susceptibility to canine visceral leishmaniasis caused by Leishmania (L.) infantum chagasi-infection |
| title_sort |
further evidence associating igg1, but not igg2, with susceptibility to canine visceral leishmaniasis caused by leishmania (l.) infantum chagasi-infection |
| publisher |
EDP Open |
| publishDate |
2017 |
| url |
http://patua.iec.gov.br//handle/iec/2858 |
| _version_ |
1717584472016158720 |
| score |
11.675088 |