Yellow Fever Virus modulates the expression of key proteins related to the microRNA pathway in the human hepatocarcinoma cell line HepG2
Yellow fever is a zoonotic disease caused by the yellow fever virus (YFV) and transmitted by mosquitoes of the family Culicidae. It is well known that cellular and viral microRNAs (miRNAs) are involved in modulation of viral and cellular gene expression, as well as immune response, and are considere...
| Autor principal: | Holanda, Gustavo Moraes |
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| Outros Autores: | Casseb, Samir Mansour Moraes, Mello, Karla Fabiane Lopes, Vasconcelos, Pedro Fernando da Costa, Cruz, Ana Cec?lia Ribeiro |
| Grau: | Artigo |
| Idioma: | eng |
| Publicado em: |
Mary Ann Liebert
2017
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| Assuntos: | |
| Acesso em linha: |
http://patua.iec.gov.br//handle/iec/2891 |
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ir-iec-2891 |
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ir-iec-28912021-05-04T13:13:56Z Yellow Fever Virus modulates the expression of key proteins related to the microRNA pathway in the human hepatocarcinoma cell line HepG2 Holanda, Gustavo Moraes Casseb, Samir Mansour Moraes Mello, Karla Fabiane Lopes Vasconcelos, Pedro Fernando da Costa Cruz, Ana Cec?lia Ribeiro V?rus da Febre Amarela / fisiologia V?rus da Febre Amarela / gen?tica C?lulas Hep G2 C?lulas Vero MicroRNAs / gen?tica Linhagem Celular Tumoral Prote?nas / metabolismo Regula??o da Express?o G?nica Carga Viral Replica??o Viral Gen?tipo Rea??o em Cadeia da Polimerase em Tempo Real / m?todos Rea??o em Cadeia da Polimerase Via Transcriptase Reversa / m?todos Yellow fever is a zoonotic disease caused by the yellow fever virus (YFV) and transmitted by mosquitoes of the family Culicidae. It is well known that cellular and viral microRNAs (miRNAs) are involved in modulation of viral and cellular gene expression, as well as immune response, and are considered by the scientific community as possible targets for an effective therapy against viral infections. This regulation may be involved in different levels of infection and clinical symptomatology. We used viral titration techniques, viral kinetics from 24 to 96 hours postinfection (hpi), and analyzed the expression of key proteins related to the miRNA pathway by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR). The expression of Dicer was different when compared over the course of infection by the distinct YFV genotypes. Drosha expression was similar during infection by YFV genotype 1 or 2, with a decrease in their expression over time and a slight increase in 96?hpi. Ago1, Ago2, and Ago4 showed different levels of expression between the viral genotypes: for YFV genotype 1 infection, Ago1 presented a positive expression, while for YFV genotype 2, it showed a negative expression, when compared with negative controls. We conclude that YFV infection modulates the proteins involved in miRNA biogenesis, which can regulate both viral replication and cellular immune response. National Institute of Science and Technology for Viral Hemorrhagic Fevers (INCT/FHV) for funding this work (process CNPq/CAPES/FAPESPA 573739/2008-0). 2017-11-29T11:22:54Z 2017-11-29T11:22:54Z 2017 Artigo HOLANDA, Gustavo Moraes et al. Yellow Fever Virus modulates the expression of key proteins related to the microRNA pathway in the human hepatocarcinoma cell line HepG2. Viral Immunology, v. 30, n. 5, p. 336-341, June 2017. 0882-8245 http://patua.iec.gov.br//handle/iec/2891 10.1089/vim.2016.0149 eng Acesso Embargado application/pdf Mary Ann Liebert |
| institution |
Instituto Evandro Chagas (IEC) |
| collection |
PATUA |
| language |
eng |
| topic |
V?rus da Febre Amarela / fisiologia V?rus da Febre Amarela / gen?tica C?lulas Hep G2 C?lulas Vero MicroRNAs / gen?tica Linhagem Celular Tumoral Prote?nas / metabolismo Regula??o da Express?o G?nica Carga Viral Replica??o Viral Gen?tipo Rea??o em Cadeia da Polimerase em Tempo Real / m?todos Rea??o em Cadeia da Polimerase Via Transcriptase Reversa / m?todos |
| spellingShingle |
V?rus da Febre Amarela / fisiologia V?rus da Febre Amarela / gen?tica C?lulas Hep G2 C?lulas Vero MicroRNAs / gen?tica Linhagem Celular Tumoral Prote?nas / metabolismo Regula??o da Express?o G?nica Carga Viral Replica??o Viral Gen?tipo Rea??o em Cadeia da Polimerase em Tempo Real / m?todos Rea??o em Cadeia da Polimerase Via Transcriptase Reversa / m?todos Holanda, Gustavo Moraes Yellow Fever Virus modulates the expression of key proteins related to the microRNA pathway in the human hepatocarcinoma cell line HepG2 |
| topic_facet |
V?rus da Febre Amarela / fisiologia V?rus da Febre Amarela / gen?tica C?lulas Hep G2 C?lulas Vero MicroRNAs / gen?tica Linhagem Celular Tumoral Prote?nas / metabolismo Regula??o da Express?o G?nica Carga Viral Replica??o Viral Gen?tipo Rea??o em Cadeia da Polimerase em Tempo Real / m?todos Rea??o em Cadeia da Polimerase Via Transcriptase Reversa / m?todos |
| description |
Yellow fever is a zoonotic disease caused by the yellow fever virus (YFV) and transmitted by mosquitoes of the family Culicidae. It is well known that cellular and viral microRNAs (miRNAs) are involved in modulation of viral and cellular gene expression, as well as immune response, and are considered by the scientific community as possible targets for an effective therapy against viral infections. This regulation may be involved in different levels of infection and clinical symptomatology. We used viral titration techniques, viral kinetics from 24 to 96 hours postinfection (hpi), and analyzed the expression of key proteins related to the miRNA pathway by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR). The expression of Dicer was different when compared over the course of infection by the distinct YFV genotypes. Drosha expression was similar during infection by YFV genotype 1 or 2, with a decrease in their expression over time and a slight increase in 96?hpi. Ago1, Ago2, and Ago4 showed different levels of expression between the viral genotypes: for YFV genotype 1 infection, Ago1 presented a positive expression, while for YFV genotype 2, it showed a negative expression, when compared with negative controls. We conclude that YFV infection modulates the proteins involved in miRNA biogenesis, which can regulate both viral replication and cellular immune response. |
| format |
Artigo |
| author |
Holanda, Gustavo Moraes |
| author2 |
Casseb, Samir Mansour Moraes Mello, Karla Fabiane Lopes Vasconcelos, Pedro Fernando da Costa Cruz, Ana Cec?lia Ribeiro |
| author2Str |
Casseb, Samir Mansour Moraes Mello, Karla Fabiane Lopes Vasconcelos, Pedro Fernando da Costa Cruz, Ana Cec?lia Ribeiro |
| title |
Yellow Fever Virus modulates the expression of key proteins related to the microRNA pathway in the human hepatocarcinoma cell line HepG2 |
| title_short |
Yellow Fever Virus modulates the expression of key proteins related to the microRNA pathway in the human hepatocarcinoma cell line HepG2 |
| title_full |
Yellow Fever Virus modulates the expression of key proteins related to the microRNA pathway in the human hepatocarcinoma cell line HepG2 |
| title_fullStr |
Yellow Fever Virus modulates the expression of key proteins related to the microRNA pathway in the human hepatocarcinoma cell line HepG2 |
| title_full_unstemmed |
Yellow Fever Virus modulates the expression of key proteins related to the microRNA pathway in the human hepatocarcinoma cell line HepG2 |
| title_sort |
yellow fever virus modulates the expression of key proteins related to the microrna pathway in the human hepatocarcinoma cell line hepg2 |
| publisher |
Mary Ann Liebert |
| publishDate |
2017 |
| url |
http://patua.iec.gov.br//handle/iec/2891 |
| _version_ |
1717584327466811392 |
| score |
11.653393 |