Leishmania amazonensis and Leishmania chagasi: in vitro leishmanicide activity of Virola surinamensis (rol.) warb
Virola species have been used in traditional medicine as healing in skin infections. From V. surinanensis oil were isolated several sesquiterpene as the nerolidol which showed activity against species of Leishmania. The current study aimed to evaluate the leishmanicide activity and toxicity of extra...
| Autor principal: | Veiga, Andreza |
|---|---|
| Outros Autores: | Albuquerque, Kelly Cristina Oliveira de, Corr?a, Maria Elinete, Brigido, Helliton, Silva, Jo?o Silva e, Campos, Marliane Batista, Silveira, Fernando Tobias, Santos, Lourivaldo, Dolabela, Maria |
| Grau: | Artigo |
| Idioma: | eng |
| Publicado em: |
Elsevier
2017
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| Assuntos: | |
| Acesso em linha: |
http://patua.iec.gov.br//handle/iec/2903 |
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ir-iec-2903 |
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dspace |
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ir-iec-29032019-08-19T19:08:54Z Leishmania amazonensis and Leishmania chagasi: in vitro leishmanicide activity of Virola surinamensis (rol.) warb Veiga, Andreza Albuquerque, Kelly Cristina Oliveira de Corr?a, Maria Elinete Brigido, Helliton Silva, Jo?o Silva e Campos, Marliane Batista Silveira, Fernando Tobias Santos, Lourivaldo Dolabela, Maria Leishmania infantum / efeitos de drogas Leishmania mexicana / efeitos de drogas Antiprotozo?rios / farmacologia Myristicaceae / qu?mica Macr?fagos Peritoneais / efeitos de drogas Macr?fagos Peritoneais / parasitologia Extratos Vegetais / farmacologia Espectroscopia de Resson?ncia Magn?tica Virola species have been used in traditional medicine as healing in skin infections. From V. surinanensis oil were isolated several sesquiterpene as the nerolidol which showed activity against species of Leishmania. The current study aimed to evaluate the leishmanicide activity and toxicity of extracts, fractions and surinamesin obtained from leaves of Virola surinamensis. Hexane, Ethyl Acetate, and Methanol extracts were obtained from powder of dry leaves of V. surinamensis. The hexane and ethyl acetate extracts were fractionated by silica gel column chromatography and increasingly polar gradient. The viability of L. chagasi and L. amazonensis promastigotes was assessed by tetrazolium salt assay (MTT). Peritoneal macrophages were exposed to L. amazonensis promastigotes. The treatment was performed with the extracts for 24 h. Then, the coverslips were stained and the infection index was determined. Cytotoxicity was determined in macrophage cells by peritoneum viability assay (MTT). The selectivity index was calculated as the product of cytotoxic concentration 50% and inhibitory concentration 50%. The hexane extract showed leishmanicide activity in promastigotes. The ethyl acetate, methanol extracts and fractions (C1-C6), were inactive against promastigote form of L. chagasi and L. amzazonensis. None extract showed effect on L. amazonensis amastigotes. All samples tested showed low cytotoxicity (CC50 > 500 ?g/mL). The selectivity index of the hexane extract was greater than 5. The hexane extract of V. surinamensis was active against L. chagasi and L. amazonensis promastigotes. The extract fractionation did not increase significantly its antipromastigote activity. The surinamensin is probably not responsible for the activity. The extracts were inactive against amastigotes of L. amazonensis. Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico, Comiss?o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES) and Funda??o ao Amaz?nia de Amparo a Estudos e Pesquisas (FAPESPA) for providing financial support for this study. 2017-12-04T14:12:22Z 2017-12-04T14:12:22Z 2017 Artigo VEIGA, Andreza et al. Leishmania amazonensis and Leishmania chagasi: in vitro leishmanicide activity of Virola surinamensis (rol.) warb. Experimental Parasitology, v. 175, p. 68-73, Apr. 2017. http://patua.iec.gov.br//handle/iec/2903 10.1016/j.exppara.2017.02.005 eng Acesso Embargado application/pdf Elsevier |
| institution |
Instituto Evandro Chagas (IEC) |
| collection |
PATUA |
| language |
eng |
| topic |
Leishmania infantum / efeitos de drogas Leishmania mexicana / efeitos de drogas Antiprotozo?rios / farmacologia Myristicaceae / qu?mica Macr?fagos Peritoneais / efeitos de drogas Macr?fagos Peritoneais / parasitologia Extratos Vegetais / farmacologia Espectroscopia de Resson?ncia Magn?tica |
| spellingShingle |
Leishmania infantum / efeitos de drogas Leishmania mexicana / efeitos de drogas Antiprotozo?rios / farmacologia Myristicaceae / qu?mica Macr?fagos Peritoneais / efeitos de drogas Macr?fagos Peritoneais / parasitologia Extratos Vegetais / farmacologia Espectroscopia de Resson?ncia Magn?tica Veiga, Andreza Leishmania amazonensis and Leishmania chagasi: in vitro leishmanicide activity of Virola surinamensis (rol.) warb |
| topic_facet |
Leishmania infantum / efeitos de drogas Leishmania mexicana / efeitos de drogas Antiprotozo?rios / farmacologia Myristicaceae / qu?mica Macr?fagos Peritoneais / efeitos de drogas Macr?fagos Peritoneais / parasitologia Extratos Vegetais / farmacologia Espectroscopia de Resson?ncia Magn?tica |
| description |
Virola species have been used in traditional medicine as healing in skin infections. From V. surinanensis oil were isolated several sesquiterpene as the nerolidol which showed activity against species of Leishmania. The current study aimed to evaluate the leishmanicide activity and toxicity of extracts, fractions and surinamesin obtained from leaves of Virola surinamensis. Hexane, Ethyl Acetate, and Methanol extracts were obtained from powder of dry leaves of V. surinamensis. The hexane and ethyl acetate extracts were fractionated by silica gel column chromatography and increasingly polar gradient. The viability of L. chagasi and L. amazonensis promastigotes was assessed by tetrazolium salt assay (MTT). Peritoneal macrophages were exposed to L. amazonensis promastigotes. The treatment was performed with the extracts for 24 h. Then, the coverslips were stained and the infection index was determined. Cytotoxicity was determined in macrophage cells by peritoneum viability assay (MTT). The selectivity index was calculated as the product of cytotoxic concentration 50% and inhibitory concentration 50%. The hexane extract showed leishmanicide activity in promastigotes. The ethyl acetate, methanol extracts and fractions (C1-C6), were inactive against promastigote form of L. chagasi and L. amzazonensis. None extract showed effect on L. amazonensis amastigotes. All samples tested showed low cytotoxicity (CC50 > 500 ?g/mL). The selectivity index of the hexane extract was greater than 5. The hexane extract of V. surinamensis was active against L. chagasi and L. amazonensis promastigotes. The extract fractionation did not increase significantly its antipromastigote activity. The surinamensin is probably not responsible for the activity. The extracts were inactive against amastigotes of L. amazonensis. |
| format |
Artigo |
| author |
Veiga, Andreza |
| author2 |
Albuquerque, Kelly Cristina Oliveira de Corr?a, Maria Elinete Brigido, Helliton Silva, Jo?o Silva e Campos, Marliane Batista Silveira, Fernando Tobias Santos, Lourivaldo Dolabela, Maria |
| author2Str |
Albuquerque, Kelly Cristina Oliveira de Corr?a, Maria Elinete Brigido, Helliton Silva, Jo?o Silva e Campos, Marliane Batista Silveira, Fernando Tobias Santos, Lourivaldo Dolabela, Maria |
| title |
Leishmania amazonensis and Leishmania chagasi: in vitro leishmanicide activity of Virola surinamensis (rol.) warb |
| title_short |
Leishmania amazonensis and Leishmania chagasi: in vitro leishmanicide activity of Virola surinamensis (rol.) warb |
| title_full |
Leishmania amazonensis and Leishmania chagasi: in vitro leishmanicide activity of Virola surinamensis (rol.) warb |
| title_fullStr |
Leishmania amazonensis and Leishmania chagasi: in vitro leishmanicide activity of Virola surinamensis (rol.) warb |
| title_full_unstemmed |
Leishmania amazonensis and Leishmania chagasi: in vitro leishmanicide activity of Virola surinamensis (rol.) warb |
| title_sort |
leishmania amazonensis and leishmania chagasi: in vitro leishmanicide activity of virola surinamensis (rol.) warb |
| publisher |
Elsevier |
| publishDate |
2017 |
| url |
http://patua.iec.gov.br//handle/iec/2903 |
| _version_ |
1717584345691062272 |
| score |
11.653393 |