Genotyping comparison of Mycobacterium leprae isolates by VNTR analysis from nasal samples in a Brazilian endemic region
This study analyzed the genetic diversity by MIRU-VNTR of Mycobacterium leprae isolates from nasal cavities and related to epidemiological and clinical data. The sample consisted of 48 newly diagnosed leprosy cases that tested positive for M. leprae PCR in nasal secretion (NS) attending to the Natio...
| Autor principal: | Lima, Luana Nepomuceno Costa |
|---|---|
| Outros Autores: | Frota, Cristiane Cunha, Suffys, Philip Noel, Fontes, Amanda Nogueira Brum, Mota, Rosa Maria Salani, Almeida, Rosa Livia Freitas, Pontes, Maria Araci de Andrade, Gon?alves, Heitor de S?, Kendall, Carl, Kerr, Ligia Regina Sansigolo |
| Grau: | Artigo |
| Idioma: | eng |
| Publicado em: |
Taylor & Francis
2018
|
| Assuntos: | |
| Acesso em linha: |
http://patua.iec.gov.br//handle/iec/3065 |
| id |
ir-iec-3065 |
|---|---|
| recordtype |
dspace |
| spelling |
ir-iec-30652019-07-04T16:11:25Z Genotyping comparison of Mycobacterium leprae isolates by VNTR analysis from nasal samples in a Brazilian endemic region Lima, Luana Nepomuceno Costa Frota, Cristiane Cunha Suffys, Philip Noel Fontes, Amanda Nogueira Brum Mota, Rosa Maria Salani Almeida, Rosa Livia Freitas Pontes, Maria Araci de Andrade Gon?alves, Heitor de S? Kendall, Carl Kerr, Ligia Regina Sansigolo Hansen?ase / diagn?stico Mycobacterium leprae / patogenicidade Cavidade Nasal / patologia Rea??o em Cadeia da Polimerase / m?todos Gen?tipo Biomarcadores This study analyzed the genetic diversity by MIRU-VNTR of Mycobacterium leprae isolates from nasal cavities and related to epidemiological and clinical data. The sample consisted of 48 newly diagnosed leprosy cases that tested positive for M. leprae PCR in nasal secretion (NS) attending to the National Reference Center of Dermatology Dona Libania (CDERM), Fortaleza, Brazil. Total DNA was extracted from NS of each patient and used for amplification of four M. leprae VNTR loci. Four clusters of M. leprae isolates were formed with identical genotypes. In the spatial analysis, 12 leprosy cases presented similar genotypes organized into 4 clusters. The most common genotypes in the current study was AC8b: 8, AC9: 7, AC8a: 8, GTA9: 10, which may represent a genotype of circulating strains most often in Cear?. A minimum set of four MIRU-VNTR loci was demonstrated to study the genetic diversity of M. leprae isolates from NS. Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico, Brazil under [grant number CNPq?410573/2006-0]. 2018-03-01T13:49:15Z 2018-03-01T13:49:15Z 2018 Artigo LIMA, Luana Nepomuceno Costa et al. Genotyping comparison of Mycobacterium leprae isolates by VNTR analysis from nasal samples in a Brazilian endemic region. Pathogens and Global Health, v. 112, n. 2, p. 79-85, Mar. 2018. 2047-7724 http://patua.iec.gov.br//handle/iec/3065 10.1080/20477724.2018.1427308 eng Acesso Aberto application/pdf Taylor & Francis |
| institution |
Instituto Evandro Chagas (IEC) |
| collection |
PATUA |
| language |
eng |
| topic |
Hansen?ase / diagn?stico Mycobacterium leprae / patogenicidade Cavidade Nasal / patologia Rea??o em Cadeia da Polimerase / m?todos Gen?tipo Biomarcadores |
| spellingShingle |
Hansen?ase / diagn?stico Mycobacterium leprae / patogenicidade Cavidade Nasal / patologia Rea??o em Cadeia da Polimerase / m?todos Gen?tipo Biomarcadores Lima, Luana Nepomuceno Costa Genotyping comparison of Mycobacterium leprae isolates by VNTR analysis from nasal samples in a Brazilian endemic region |
| topic_facet |
Hansen?ase / diagn?stico Mycobacterium leprae / patogenicidade Cavidade Nasal / patologia Rea??o em Cadeia da Polimerase / m?todos Gen?tipo Biomarcadores |
| description |
This study analyzed the genetic diversity by MIRU-VNTR of Mycobacterium leprae isolates from nasal cavities and related to epidemiological and clinical data. The sample consisted of 48 newly diagnosed leprosy cases that tested positive for M. leprae PCR in nasal secretion (NS) attending to the National Reference Center of Dermatology Dona Libania (CDERM), Fortaleza, Brazil. Total DNA was extracted from NS of each patient and used for amplification of four M. leprae VNTR loci. Four clusters of M. leprae isolates were formed with identical genotypes. In the spatial analysis, 12 leprosy cases presented similar genotypes organized into 4 clusters. The most common genotypes in the current study was AC8b: 8, AC9: 7, AC8a: 8, GTA9: 10, which may represent a genotype of circulating strains most often in Cear?. A minimum set of four MIRU-VNTR loci was demonstrated to study the genetic diversity of M. leprae isolates from NS. |
| format |
Artigo |
| author |
Lima, Luana Nepomuceno Costa |
| author2 |
Frota, Cristiane Cunha Suffys, Philip Noel Fontes, Amanda Nogueira Brum Mota, Rosa Maria Salani Almeida, Rosa Livia Freitas Pontes, Maria Araci de Andrade Gon?alves, Heitor de S? Kendall, Carl Kerr, Ligia Regina Sansigolo |
| author2Str |
Frota, Cristiane Cunha Suffys, Philip Noel Fontes, Amanda Nogueira Brum Mota, Rosa Maria Salani Almeida, Rosa Livia Freitas Pontes, Maria Araci de Andrade Gon?alves, Heitor de S? Kendall, Carl Kerr, Ligia Regina Sansigolo |
| title |
Genotyping comparison of Mycobacterium leprae isolates by VNTR analysis from nasal samples in a Brazilian endemic region |
| title_short |
Genotyping comparison of Mycobacterium leprae isolates by VNTR analysis from nasal samples in a Brazilian endemic region |
| title_full |
Genotyping comparison of Mycobacterium leprae isolates by VNTR analysis from nasal samples in a Brazilian endemic region |
| title_fullStr |
Genotyping comparison of Mycobacterium leprae isolates by VNTR analysis from nasal samples in a Brazilian endemic region |
| title_full_unstemmed |
Genotyping comparison of Mycobacterium leprae isolates by VNTR analysis from nasal samples in a Brazilian endemic region |
| title_sort |
genotyping comparison of mycobacterium leprae isolates by vntr analysis from nasal samples in a brazilian endemic region |
| publisher |
Taylor & Francis |
| publishDate |
2018 |
| url |
http://patua.iec.gov.br//handle/iec/3065 |
| _version_ |
1717584372368932864 |
| score |
11.755432 |