Evaluation of third-generation RIDASCREEN enzyme immunoassay for the detection of norovirus antigens in stool samples of hospitalized children in Bel?m, Par?, Brazil
Noroviruses (NoVs) are major agents of gastroenteritis outbreaks and hospitalization worldwide. This study evaluated the sensitivity and specificity of the commercially available third-generation RIDASCREEN? Norovirus Enzyme Immunoassay (EIA) kit in comparison to the reverse transcription-polymera...
| Autor principal: | Siqueira, Jones Anderson Monteiro |
|---|---|
| Outros Autores: | Linhares, Alexandre da Costa, Oliveira, Darleise de Souza, Soares, Luana da Silva, Lucena, Maria Silvia Souza, Wanzeller, Ana L?cia Monteiro, Mascarenhas, Joana D'Arc Pereira, Gabbay, Yvone Benchimol |
| Grau: | Artigo |
| Idioma: | eng |
| Publicado em: |
Elsevier
2018
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| Assuntos: | |
| Acesso em linha: |
http://patua.iec.gov.br//handle/iec/3297 |
| Resumo: |
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Noroviruses (NoVs) are major agents of gastroenteritis outbreaks and hospitalization worldwide. This study evaluated the sensitivity and
specificity of the commercially available third-generation RIDASCREEN? Norovirus Enzyme Immunoassay (EIA) kit in comparison to the
reverse transcription-polymerase chain reaction (RT-PCR) to detect NoVs in hospitalized children with gastroenteritis. An agreement of 88%
(81/92) was observed when comparing EIA with RT-PCR. A sensitivity of 92% and a specificity of 83.3% were demonstrated. Eleven
samples were positive by 1 method only (4 RT-PCR/7 EIA). Fourteen samples were sequenced and all classified as NoV genogroup GII-4.
The 7 positive only by EIA were also evaluated by electron microscopy, and in 3 (42.9%) samples viral particles with a suggestive
morphology of NoVs were visualized. These same samples were tested by seminested-RT-PCR with a positivity of 85.7%. The results
obtained in this study demonstrated a significant improvement in the sensitivity and specificity of this updated assay |