A single-dose live-attenuated Zika Virus vaccine with controlled infection rounds that protects against vertical transmission
Zika virus (ZIKV) infection of the mother during pregnancy causes devastating Zika congenital syndrome in the offspring. A ZIKV vaccine with optimal safety and immunogenicity for use in pregnant women is critically needed. Toward this goal, we have developed a single-dose live-attenuated vaccine can...
| Autor principal: | Xie, Xuping |
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| Outros Autores: | Kum, Dieudonn? B, Xia, Hongjie, Luo, Huanle, Shan, Chao, Zou, Jing, Muruato, Antonio E, Medeiros, Daniele Barbosa de Almeida, Nunes, Bruno Tardelli Diniz, Dallmeier, Kai, Rossi, Shannan L, Weaver, Scott C, Neyts, Johan, Wang, Tian, Vasconcelos, Pedro Fernando da Costa, Shi, Pei-Yong |
| Grau: | Artigo |
| Idioma: | eng |
| Publicado em: |
Elsevier
2018
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| Acesso em linha: |
http://patua.iec.gov.br//handle/iec/3517 |
| Resumo: |
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Zika virus (ZIKV) infection of the mother during pregnancy causes devastating Zika congenital syndrome in the offspring. A ZIKV vaccine with optimal safety and immunogenicity for use in pregnant women is critically needed. Toward this goal, we have developed a single-dose live-attenuated vaccine candidate that infects cells with controlled, limited infection rounds. The vaccine contains a 9-amino-acid deletion in the viral capsid protein and replicates to titers of > 10 6 focus-forming units (FFU)/mL in cells expressing the full-length capsid protein. Immunization of A129 mice with one dose (10 5 FFU) did not produce viremia, but elicited protective immunity that completely prevented viremia, morbidity, and mortality after challenge with an epidemic ZIKV strain (10 6 PFU). A single-dose vaccination also fully prevented infection of pregnant mice and maternal-to-fetal transmission. Intracranial injection of the vaccine (10 4 FFU) to 1-day-old mice did not cause any disease or death, underscoring the safety of this vaccine candidate. |