Enteric adenovirus (AdV), sapovirus (SaV), and human astrovirus (HAstV) are
important pathogens involved in the gastroenteritis etiology. In this study, a total of
219 fecal samples and sera were collected from children hospitalized for acute
gastroenteritis (AGE) in two large pediatric hospitals in Bel?m, from March 2012
to April 2015. The samples were analyzed by polymerase chain reaction (PCR) for
adenovirus (AdV) and HAstV (astrovirus) detection, and Nested-PCR and qPCR
for sapovirus (SaV) detection. AdV was detected in 50.2% (110/219) of the cases,
with 42.7% (47/110) being sequenced and classified as: species F (63.9% - 30/47),
A (4.2% - 2/47), B (6.4% - 3/47), C (17.1% - 8/47), D (4.2% - 2/47), and E (4.2% -
2/47). Of the 110 AdV-positive faeces samples, 80 paired sera presented sufficient
amounts and were also tested for this virus, of which 51 (63.7%) showed positive
results and 26 (70.3%) pairs (feces plus sera) presented concordant results after
sequencing being classified as: species F (21/26; 80.8%), A (1/26; 3.8%), B (1/26;
3.8%), and C (3/26; 11.5%). Overall, HAstV rate in the feces samples was 1.8%
(4/219), including both HAstV-1a (2/4; 50%) and HAstV-2c (2/4; 50%). SaV was
detected in 4.6% (10/219) of the fecal samples, out of which 50% (5/10) of the
positive samples were characterized into the genogroups GI.1 (1), GI.2 (2), and
GII.4 (2). These findings highlighted the important contributions of AdV, HAstV,
and SaV in the enteric virus spectrum in our region and showed the high genetic
diversity of AdV. In addition, it demonstrated for the first time in Brazil, the
circulation of AdV in the serum of hospitalized children with AGE.
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