Artigo

In vitro antileishmanial effects of Physalis angulata root extract on Leishmania infantum

OBJECTIVE: In the present study, we evaluated the effects of the aqueous extract of Physalis angulata root (AEPa) on Leishmania infantum proliferation, morphology, and the driving mechanism in leishmanicidal activity and modulatory action on macrophages. METHODS: L. infantum promastigotes were treat...

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Autor principal: Silva, Bruno Jos? Martins da
Outros Autores: Pereira, Sandro Wilson Gomes, Rodrigues, Ana Paula Drummond, Nascimento, Jos? Luiz Martins do, Silva, Edilene Oliveira
Grau: Artigo
Idioma: eng
Publicado em: Elsevier 2018
Assuntos:
Leishmania infantum / patogenicidade
Extratos Vegetais
Plantas Medicinais
Macr?fagos / citologia
Physalis angulata
Acesso em linha: http://patua.iec.gov.br//handle/iec/3534
id ir-iec-3534
recordtype dspace
spelling ir-iec-35342018-11-22T16:39:06Z In vitro antileishmanial effects of Physalis angulata root extract on Leishmania infantum Silva, Bruno Jos? Martins da Pereira, Sandro Wilson Gomes Rodrigues, Ana Paula Drummond Nascimento, Jos? Luiz Martins do Silva, Edilene Oliveira Leishmania infantum / patogenicidade Extratos Vegetais Plantas Medicinais Macr?fagos / citologia Physalis angulata OBJECTIVE: In the present study, we evaluated the effects of the aqueous extract of Physalis angulata root (AEPa) on Leishmania infantum proliferation, morphology, and the driving mechanism in leishmanicidal activity and modulatory action on macrophages. METHODS: L. infantum promastigotes were treated with 50 and 100??g/mL AEPa for 72?h and then antipromastigote assay was performed by counts in a Newbauer chamber, morphological changes were analyzed by transmission electron microscopy and the mechanism of the leishmanicidal activity was detected. In addition, macrophages were infected with L. infantum and were used to evaluate anti-amastigote activity of AEPa and effects of AEPa on cytokine secretion after 72-hour treatment. RESULTS: Treatment with AEPa reduced the numbers of L. infantum promastigotes (50% inhibitory concentration (IC50)?=?65.9??g/mL; selectivity index (SI)?=?22.1) and amastigotes (IC50?=?37.9??g/mL; SI?=?38.5) compared with the untreated control. Amphotericin B reduced 100% of the promastigote numbers after 72?h of treatment (IC50?=?0.2??g/mL). AEPa induced several morphological changes and increased the production of reactive oxygen species and apoptotic death in promastigotes after treating for 72?h. AEPa (100??g/mL) promoted tumor necrosis factor-? secretion in macrophages infected with L. infantum after 72?h of treatment, but did not induce an increase in this cytokine in noninfected macrophages. In addition, AEPa showed no cytotoxic effect on J774-A1 cells (50% cytotoxic concentration >1000??g/mL). CONCLUSION: AEPa presented antileishmanial activity against the promastigotes and amastigotes of L. infantum without macrophage cytotoxicity; these results show that natural products such as P. angulata have leishmanicidal potential and in the future may be an alternative treatment for leishmaniasis 2018-11-22T14:25:34Z 2018-11-22T14:25:34Z 2018 Artigo SILVA, Bruno Jos? Martins da et al. In vitro antileishmanial effects of Physalis angulata root extract on Leishmania infantum. Journal of Integrative Medicine, v. 16, n. 6, p. 404-410, Nov. 2018. 2095-4964 http://patua.iec.gov.br//handle/iec/3534 10.1016/j.joim.2018.08.004 eng Acesso Embargado application/pdf Elsevier
institution Instituto Evandro Chagas (IEC)
collection PATUA
language eng
topic Leishmania infantum / patogenicidade
Extratos Vegetais
Plantas Medicinais
Macr?fagos / citologia
Physalis angulata
spellingShingle Leishmania infantum / patogenicidade
Extratos Vegetais
Plantas Medicinais
Macr?fagos / citologia
Physalis angulata
Silva, Bruno Jos? Martins da
In vitro antileishmanial effects of Physalis angulata root extract on Leishmania infantum
topic_facet Leishmania infantum / patogenicidade
Extratos Vegetais
Plantas Medicinais
Macr?fagos / citologia
Physalis angulata
description OBJECTIVE: In the present study, we evaluated the effects of the aqueous extract of Physalis angulata root (AEPa) on Leishmania infantum proliferation, morphology, and the driving mechanism in leishmanicidal activity and modulatory action on macrophages. METHODS: L. infantum promastigotes were treated with 50 and 100??g/mL AEPa for 72?h and then antipromastigote assay was performed by counts in a Newbauer chamber, morphological changes were analyzed by transmission electron microscopy and the mechanism of the leishmanicidal activity was detected. In addition, macrophages were infected with L. infantum and were used to evaluate anti-amastigote activity of AEPa and effects of AEPa on cytokine secretion after 72-hour treatment. RESULTS: Treatment with AEPa reduced the numbers of L. infantum promastigotes (50% inhibitory concentration (IC50)?=?65.9??g/mL; selectivity index (SI)?=?22.1) and amastigotes (IC50?=?37.9??g/mL; SI?=?38.5) compared with the untreated control. Amphotericin B reduced 100% of the promastigote numbers after 72?h of treatment (IC50?=?0.2??g/mL). AEPa induced several morphological changes and increased the production of reactive oxygen species and apoptotic death in promastigotes after treating for 72?h. AEPa (100??g/mL) promoted tumor necrosis factor-? secretion in macrophages infected with L. infantum after 72?h of treatment, but did not induce an increase in this cytokine in noninfected macrophages. In addition, AEPa showed no cytotoxic effect on J774-A1 cells (50% cytotoxic concentration >1000??g/mL). CONCLUSION: AEPa presented antileishmanial activity against the promastigotes and amastigotes of L. infantum without macrophage cytotoxicity; these results show that natural products such as P. angulata have leishmanicidal potential and in the future may be an alternative treatment for leishmaniasis
format Artigo
author Silva, Bruno Jos? Martins da
author2 Pereira, Sandro Wilson Gomes
Rodrigues, Ana Paula Drummond
Nascimento, Jos? Luiz Martins do
Silva, Edilene Oliveira
author2Str Pereira, Sandro Wilson Gomes
Rodrigues, Ana Paula Drummond
Nascimento, Jos? Luiz Martins do
Silva, Edilene Oliveira
title In vitro antileishmanial effects of Physalis angulata root extract on Leishmania infantum
title_short In vitro antileishmanial effects of Physalis angulata root extract on Leishmania infantum
title_full In vitro antileishmanial effects of Physalis angulata root extract on Leishmania infantum
title_fullStr In vitro antileishmanial effects of Physalis angulata root extract on Leishmania infantum
title_full_unstemmed In vitro antileishmanial effects of Physalis angulata root extract on Leishmania infantum
title_sort in vitro antileishmanial effects of physalis angulata root extract on leishmania infantum
publisher Elsevier
publishDate 2018
url http://patua.iec.gov.br//handle/iec/3534
_version_ 1717584531844759552
score 11.653393

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