The isotype composition and avidity of naturally acquired anti-plasmodium falciparum antibodies: differential patterns in clinically immune africans and amazonian patients
A critical role has been proposed for cytophilic IgOl and IgOJ subclass antibodies and monocytes and macrophages in antimalarial immunity. Here we compared lhe isotype composition and avidity of naturally acquired antibodies, as measured by enzyme immunoassay against a detergent-soluble extract of...
| Autor principal: | Ferreira, Marcelo Urbano |
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| Outros Autores: | Kimura, Emilia Akemi Shiraishi, Souza, Jos? Maria de, Katzin, Alejandro Miguel |
| Grau: | Artigo |
| Idioma: | eng |
| Publicado em: |
American Society of Tropical Medicine and Hygiene
2019
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| Assuntos: | |
| Acesso em linha: |
http://patua.iec.gov.br//handle/iec/3563 |
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ir-iec-3563 |
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dspace |
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ir-iec-35632019-01-22T13:15:29Z The isotype composition and avidity of naturally acquired anti-plasmodium falciparum antibodies: differential patterns in clinically immune africans and amazonian patients Ferreira, Marcelo Urbano Kimura, Emilia Akemi Shiraishi Souza, Jos? Maria de Katzin, Alejandro Miguel Mal?ria Plasmodium falciparum / parasitologia T?cnicas Imunoenzim?ticas / m?todos A critical role has been proposed for cytophilic IgOl and IgOJ subclass antibodies and monocytes and macrophages in antimalarial immunity. Here we compared lhe isotype composition and avidity of naturally acquired antibodies, as measured by enzyme immunoassay against a detergent-soluble extract of Plasmodium falciparum schizonts, in clinically immune Senegalese adults (n = 33) and semi-immune, adult Amazonian patients (n = 25). Plasma were collected during an acute symptomatic P. falciparum attack and two months later, and in the absence of recrudescence or reinfection. Specific IgO, IgM, IgA, and IgO subclass antibodies were assessed. The results are summarized as follows: 1) high-avidity cytophilic antibodies predorninated in clinically immune Senegalese subjects; 2) acutely ill Amazonian patients produced high levels of low-avidity cytophilic antibody; 3) such a response was shonlived, since two months later, the concentrations of cytophilic antibodies were significantly lower; 4) however, affinity maturation of IgO antibodies was observed in Amazonian patients two months after the acute malaria attack. A considerable proponion (35-46%) of anti-P. falciparum IgOI antibodies produced by African and Amazonian patients was shown to recognize periodate-sensitive carbohydrate epitopes. The potential impact of these findings on the design and evaluation of antimalarial vaccines is discussed. This work was supported by grants from the UNDP/World Bank/World Health Organization Special Program for Research and Training in Tropical Diseases, the Program for Malaria Contrai in the Amazon (PCMAM) of the National Health Foundation (Brazilian Ministry of Health), and the Fundacao para o Amparo a Pesquisa do Estado de Sao Paulo (FAPESP), Brazil. 2019-01-22T13:03:03Z 2019-01-22T13:03:03Z 1996 Artigo FERREIRA, Marcelo Urbano et al. The isotype composition and avidity of naturally acquired anti-plasmodium falciparum antibodies: differential patterns in clinically immune africans and amazonian patients. American Journal of Tropical Medicine and Hygiene, v. 55, n. 3, p. 315-323, Sept. 1996. 0002-9637 http://patua.iec.gov.br//handle/iec/3563 doi.org/10.4269/ajtmh.1996.55.315 eng Acesso Aberto application/pdf American Society of Tropical Medicine and Hygiene |
| institution |
Instituto Evandro Chagas (IEC) |
| collection |
PATUA |
| language |
eng |
| topic |
Mal?ria Plasmodium falciparum / parasitologia T?cnicas Imunoenzim?ticas / m?todos |
| spellingShingle |
Mal?ria Plasmodium falciparum / parasitologia T?cnicas Imunoenzim?ticas / m?todos Ferreira, Marcelo Urbano The isotype composition and avidity of naturally acquired anti-plasmodium falciparum antibodies: differential patterns in clinically immune africans and amazonian patients |
| topic_facet |
Mal?ria Plasmodium falciparum / parasitologia T?cnicas Imunoenzim?ticas / m?todos |
| description |
A critical role has been proposed for cytophilic IgOl and IgOJ subclass antibodies and monocytes and
macrophages in antimalarial immunity. Here we compared lhe isotype composition and avidity of naturally acquired
antibodies, as measured by enzyme immunoassay against a detergent-soluble extract of Plasmodium falciparum schizonts, in clinically immune Senegalese adults (n = 33) and semi-immune, adult Amazonian patients (n = 25). Plasma
were collected during an acute symptomatic P. falciparum attack and two months later, and in the absence of recrudescence or reinfection. Specific IgO, IgM, IgA, and IgO subclass antibodies were assessed. The results are summarized as follows: 1) high-avidity cytophilic antibodies predorninated in clinically immune Senegalese subjects; 2)
acutely ill Amazonian patients produced high levels of low-avidity cytophilic antibody; 3) such a response was shonlived, since two months later, the concentrations of cytophilic antibodies were significantly lower; 4) however, affinity
maturation of IgO antibodies was observed in Amazonian patients two months after the acute malaria attack. A
considerable proponion (35-46%) of anti-P. falciparum IgOI antibodies produced by African and Amazonian patients
was shown to recognize periodate-sensitive carbohydrate epitopes. The potential impact of these findings on the
design and evaluation of antimalarial vaccines is discussed. |
| format |
Artigo |
| author |
Ferreira, Marcelo Urbano |
| author2 |
Kimura, Emilia Akemi Shiraishi Souza, Jos? Maria de Katzin, Alejandro Miguel |
| author2Str |
Kimura, Emilia Akemi Shiraishi Souza, Jos? Maria de Katzin, Alejandro Miguel |
| title |
The isotype composition and avidity of naturally acquired anti-plasmodium falciparum antibodies: differential patterns in clinically immune africans and amazonian patients |
| title_short |
The isotype composition and avidity of naturally acquired anti-plasmodium falciparum antibodies: differential patterns in clinically immune africans and amazonian patients |
| title_full |
The isotype composition and avidity of naturally acquired anti-plasmodium falciparum antibodies: differential patterns in clinically immune africans and amazonian patients |
| title_fullStr |
The isotype composition and avidity of naturally acquired anti-plasmodium falciparum antibodies: differential patterns in clinically immune africans and amazonian patients |
| title_full_unstemmed |
The isotype composition and avidity of naturally acquired anti-plasmodium falciparum antibodies: differential patterns in clinically immune africans and amazonian patients |
| title_sort |
isotype composition and avidity of naturally acquired anti-plasmodium falciparum antibodies: differential patterns in clinically immune africans and amazonian patients |
| publisher |
American Society of Tropical Medicine and Hygiene |
| publishDate |
2019 |
| url |
http://patua.iec.gov.br//handle/iec/3563 |
| _version_ |
1717584374748151808 |
| score |
11.755432 |