Association of polymorphisms of the ?-defensin 1 gene with nematode and protozoan infection traits in goat
This study's purpose was to identify polymorphisms (SNP) in the goat ?-defensin 1 gene and to associate these SNPs with traits related to nematodean and protozoan infections in Anglo-Nubian goats from semiarid region of Brazil. A total of 184 animals were used for DNA extraction, PCR and DNA automat...
| Autor principal: | Maia, Flora Suzane Parente |
|---|---|
| Outros Autores: | Campelo, Jos? Elivalto Guimar?es, Sarmento, Jos? Lindemberg Rocha, Silva, Caio Santos, Marques, Jos? Ribamar Felipe, Alves, Francisco Arimat?ia Santos, Guimar?es, Rafaelle Casseb, Silva Filho, Ednaldo |
| Grau: | Artigo |
| Idioma: | eng |
| Publicado em: |
Wiley
2019
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| Assuntos: | |
| Acesso em linha: |
http://patua.iec.gov.br//handle/iec/3592 |
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ir-iec-3592 |
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dspace |
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ir-iec-35922019-02-18T16:44:24Z Association of polymorphisms of the ?-defensin 1 gene with nematode and protozoan infection traits in goat Maia, Flora Suzane Parente Campelo, Jos? Elivalto Guimar?es Sarmento, Jos? Lindemberg Rocha Silva, Caio Santos Marques, Jos? Ribamar Felipe Alves, Francisco Arimat?ia Santos Guimar?es, Rafaelle Casseb Silva Filho, Ednaldo Polimorfismo Gen?tico / gen?tica Infec??es por Nematoides Infec??es por Protozo?rios Cabras / parasitologia This study's purpose was to identify polymorphisms (SNP) in the goat ?-defensin 1 gene and to associate these SNPs with traits related to nematodean and protozoan infections in Anglo-Nubian goats from semiarid region of Brazil. A total of 184 animals were used for DNA extraction, PCR and DNA automatic sequencing. The association analyses included the fixed effects of animal age, bloodline and genotype of the SNP marker in the statistical model. The means of genotypes were compared by the Fisher test (P < 0.05). Twelve polymorphism genotypes were found: two in intron 1, seven in exon 2 and three in 3' untranslated region. The SNPs of exon 2 were responsible for amino acid substitutions in six genetic codes, and the changes in the 25th and 33rd codes affected the protein function. The SNP 1937 was significantly associated with number of protozoan oocysts, whereas SNP 2001 was associated with degree of anaemia (Famacha?). Polymorphism 2046, in turn, showed a significant association with Famacha? degree and number of protozoan oocysts. SNP 2140 associated with maximum EPG of the animal. Results from this study suggest that the ?-defensin 1 gene can be used as a molecular marker for selection of goats regarding the susceptibility to endoparasites infections. 2019-02-06T17:32:00Z 2019-02-06T17:32:00Z 2018 Artigo MAIA, Flora Suzane Parente et al. Association of polymorphisms of the ?-defensin 1 gene with nematode and protozoan infection traits in goat. Parasite Immunology, v. xx. e12613, n. xx, p. xx, Dec. 2018.(Epub ahead of prin) 0141-9838 http://patua.iec.gov.br//handle/iec/3592 10.1111/pim.12613 eng Acesso Aberto application/pdf Wiley |
| institution |
Instituto Evandro Chagas (IEC) |
| collection |
PATUA |
| language |
eng |
| topic |
Polimorfismo Gen?tico / gen?tica Infec??es por Nematoides Infec??es por Protozo?rios Cabras / parasitologia |
| spellingShingle |
Polimorfismo Gen?tico / gen?tica Infec??es por Nematoides Infec??es por Protozo?rios Cabras / parasitologia Maia, Flora Suzane Parente Association of polymorphisms of the ?-defensin 1 gene with nematode and protozoan infection traits in goat |
| topic_facet |
Polimorfismo Gen?tico / gen?tica Infec??es por Nematoides Infec??es por Protozo?rios Cabras / parasitologia |
| description |
This study's purpose was to identify polymorphisms (SNP) in the goat ?-defensin 1 gene and to associate these SNPs with traits related to nematodean and protozoan infections in Anglo-Nubian goats from semiarid region of Brazil. A total of 184 animals were used for DNA extraction, PCR and DNA automatic sequencing. The association analyses included the fixed effects of animal age, bloodline and genotype of the SNP marker in the statistical model. The means of genotypes were compared by the Fisher test (P < 0.05). Twelve polymorphism genotypes were found: two in intron 1, seven in exon 2 and three in 3' untranslated region. The SNPs of exon 2 were responsible for amino acid substitutions in six genetic codes, and the changes in the 25th and 33rd codes affected the protein function. The SNP 1937 was significantly associated with number of protozoan oocysts, whereas SNP 2001 was associated with degree of anaemia (Famacha?). Polymorphism 2046, in turn, showed a significant association with Famacha? degree and number of protozoan oocysts. SNP 2140 associated with maximum EPG of the animal. Results from this study suggest that the ?-defensin 1 gene can be used as a molecular marker for selection of goats regarding the susceptibility to endoparasites infections. |
| format |
Artigo |
| author |
Maia, Flora Suzane Parente |
| author2 |
Campelo, Jos? Elivalto Guimar?es Sarmento, Jos? Lindemberg Rocha Silva, Caio Santos Marques, Jos? Ribamar Felipe Alves, Francisco Arimat?ia Santos Guimar?es, Rafaelle Casseb Silva Filho, Ednaldo |
| author2Str |
Campelo, Jos? Elivalto Guimar?es Sarmento, Jos? Lindemberg Rocha Silva, Caio Santos Marques, Jos? Ribamar Felipe Alves, Francisco Arimat?ia Santos Guimar?es, Rafaelle Casseb Silva Filho, Ednaldo |
| title |
Association of polymorphisms of the ?-defensin 1 gene with nematode and protozoan infection traits in goat |
| title_short |
Association of polymorphisms of the ?-defensin 1 gene with nematode and protozoan infection traits in goat |
| title_full |
Association of polymorphisms of the ?-defensin 1 gene with nematode and protozoan infection traits in goat |
| title_fullStr |
Association of polymorphisms of the ?-defensin 1 gene with nematode and protozoan infection traits in goat |
| title_full_unstemmed |
Association of polymorphisms of the ?-defensin 1 gene with nematode and protozoan infection traits in goat |
| title_sort |
association of polymorphisms of the ?-defensin 1 gene with nematode and protozoan infection traits in goat |
| publisher |
Wiley |
| publishDate |
2019 |
| url |
http://patua.iec.gov.br//handle/iec/3592 |
| _version_ |
1717584349038116864 |
| score |
11.675608 |