Association of Chlamydia trachomatis, C. pneumoniae, and IL-6 and IL-8 Gene alterations with heart diseases
Atherosclerosis is a progressive disease characterized by chronic inflammation of the arterial walls, associated with genetic and infectious factors. The present study investigated the involvement of Chlamydia trachomatis and Chlamydia pneumoniae infections and immunological markers (C-reactive prot...
| Autor principal: | Almeida, Nubia Caroline Costa |
|---|---|
| Outros Autores: | Queiroz, Maria Alice Freitas, Lima, Sandra Souza, Costa, Igor Brasil, Fossa, Marco Antonio Ayin, Vallinoto, Antonio Carlos Ros?rio, Ishak, Marlu?sa de Oliveira Guimar?es, Ishak, Ricardo |
| Grau: | Artigo |
| Idioma: | eng |
| Publicado em: |
Frontiers Media
2019
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| Assuntos: | |
| Acesso em linha: |
http://patua.iec.gov.br//handle/iec/3594 |
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ir-iec-3594 |
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ir-iec-35942019-02-18T18:39:38Z Association of Chlamydia trachomatis, C. pneumoniae, and IL-6 and IL-8 Gene alterations with heart diseases Almeida, Nubia Caroline Costa Queiroz, Maria Alice Freitas Lima, Sandra Souza Costa, Igor Brasil Fossa, Marco Antonio Ayin Vallinoto, Antonio Carlos Ros?rio Ishak, Marlu?sa de Oliveira Guimar?es Ishak, Ricardo Sistema Cardiovascular Aterosclerose Vasos Coron?rios / anatomia & histologia Valva A?rtica / anatomia & histologia Chlamydia trachomatis / patogenicidade Chlamydophila pneumoniae / patogenicidade Biomarcadores / an?lise Infec??es por Chlamydia / patogenicidade Atherosclerosis is a progressive disease characterized by chronic inflammation of the arterial walls, associated with genetic and infectious factors. The present study investigated the involvement of Chlamydia trachomatis and Chlamydia pneumoniae infections and immunological markers (C-reactive protein, CRP, TNF-alpha, IL-6, IL-8, and IL-10) in the process of atherosclerosis. The evaluation included 159 patients for surgical revascularization (CAD) and 71 patients for surgical heart valve disease (HVD) at three hospitals in Belem, Brazil. The control group (CG) comprised 300 healthy individuals. Blood samples collected before surgery were used for antibodies detection (enzyme immunoassay), CRP (immunoturbidimetry) and IL-6 levels (enzyme immunoassay). Tissue fragments (atheroma plaque, heart valve and ascending aorta) were collected during surgery and subjected to qPCR for detection of bacterial DNA. Promoter region polymorphisms of eachmarker and relative quantification of TNF-alpha, IL-8, and IL-10 gene expression were performed. Demography and social information were similar to the general population involved with both diseases. Antibody prevalence to C. trachomatis was 30.6, 20.3, and 36.7% (in the CAD, HVD, and CG, respectively) and to C. pneumoniae was 83.6, 84.5, and 80.3% (in the CAD, HVD, and CG, respectively). C. trachomatis cryptic plasmid DNA was detected in 7.4% of the samples. Frequency of IL6-174G > C polymorphism was higher in CAD and HVD than in CG regardless of previous exposure to Chlamydia. Previous C. trachomatis infection showed involvement in HVD and CAD. Significant association between disease and previous C. pneumoniae infection was found only among HVD. GG genotype of IL6-174G > C is apparently a risk factor for heart disease, whereas AT genotype of IL8-251A > T was mainly involved in valvulopathies, including patients with prior exposure to C. pneumoniae. Conselho Nacional de Desenvolvimento Cientifico e Tecnologico/Programa de Apoio a Nucleos de Excelencia/Fundacao Amazonia de Amparo a Estudos e Pesquisas do Para (CNPq/PRONEX/FAPESPA) ICAAF092/2010; Pro-reitoria de Pesquisa e Pos-Graduacao-PROPESP/UFPA (PAPQ-2018) 2019-02-18T18:03:47Z 2019-02-18T18:03:47Z 2019 Artigo ALMEIDA, Nubia Caroline Costa et al. Association of Chlamydia trachomatis, C. pneumoniae, and IL-6 and IL-8 Gene alterations with heart diseases. Frontiers in Immunology, v. 10 n. 87, Feb. 2019. 1664-3224 http://patua.iec.gov.br//handle/iec/3594 10.3389/fimmu.2019.00087 eng Acesso Aberto application/pdf Frontiers Media |
| institution |
Instituto Evandro Chagas (IEC) |
| collection |
PATUA |
| language |
eng |
| topic |
Sistema Cardiovascular Aterosclerose Vasos Coron?rios / anatomia & histologia Valva A?rtica / anatomia & histologia Chlamydia trachomatis / patogenicidade Chlamydophila pneumoniae / patogenicidade Biomarcadores / an?lise Infec??es por Chlamydia / patogenicidade |
| spellingShingle |
Sistema Cardiovascular Aterosclerose Vasos Coron?rios / anatomia & histologia Valva A?rtica / anatomia & histologia Chlamydia trachomatis / patogenicidade Chlamydophila pneumoniae / patogenicidade Biomarcadores / an?lise Infec??es por Chlamydia / patogenicidade Almeida, Nubia Caroline Costa Association of Chlamydia trachomatis, C. pneumoniae, and IL-6 and IL-8 Gene alterations with heart diseases |
| topic_facet |
Sistema Cardiovascular Aterosclerose Vasos Coron?rios / anatomia & histologia Valva A?rtica / anatomia & histologia Chlamydia trachomatis / patogenicidade Chlamydophila pneumoniae / patogenicidade Biomarcadores / an?lise Infec??es por Chlamydia / patogenicidade |
| description |
Atherosclerosis is a progressive disease characterized by chronic inflammation of the arterial walls, associated with genetic and infectious factors. The present study investigated the involvement of Chlamydia trachomatis and Chlamydia pneumoniae infections and immunological markers (C-reactive protein, CRP, TNF-alpha, IL-6, IL-8, and IL-10) in the process of atherosclerosis. The evaluation included 159 patients for surgical revascularization (CAD) and 71 patients for surgical heart valve disease (HVD) at three hospitals in Belem, Brazil. The control group (CG) comprised 300 healthy individuals. Blood samples collected before surgery were used for antibodies detection (enzyme immunoassay), CRP (immunoturbidimetry) and IL-6 levels (enzyme immunoassay). Tissue fragments (atheroma plaque, heart valve and ascending aorta) were collected during surgery and subjected to qPCR for detection of bacterial DNA. Promoter region polymorphisms of eachmarker and relative quantification of TNF-alpha, IL-8, and IL-10 gene expression were performed. Demography and social information were similar to the general population involved with both diseases. Antibody prevalence to C. trachomatis was 30.6, 20.3, and 36.7% (in the CAD, HVD, and CG, respectively) and to C. pneumoniae was 83.6, 84.5, and 80.3% (in the CAD, HVD, and CG, respectively). C. trachomatis cryptic plasmid DNA was detected in 7.4% of the samples. Frequency of IL6-174G > C polymorphism was higher in CAD and HVD than in CG regardless of previous exposure to Chlamydia. Previous C. trachomatis infection showed involvement in HVD and CAD. Significant association between disease and previous C. pneumoniae infection was found only among HVD. GG genotype of IL6-174G > C is apparently a risk factor for heart disease, whereas AT genotype of IL8-251A > T was mainly involved in valvulopathies, including patients with prior exposure to C. pneumoniae. |
| format |
Artigo |
| author |
Almeida, Nubia Caroline Costa |
| author2 |
Queiroz, Maria Alice Freitas Lima, Sandra Souza Costa, Igor Brasil Fossa, Marco Antonio Ayin Vallinoto, Antonio Carlos Ros?rio Ishak, Marlu?sa de Oliveira Guimar?es Ishak, Ricardo |
| author2Str |
Queiroz, Maria Alice Freitas Lima, Sandra Souza Costa, Igor Brasil Fossa, Marco Antonio Ayin Vallinoto, Antonio Carlos Ros?rio Ishak, Marlu?sa de Oliveira Guimar?es Ishak, Ricardo |
| title |
Association of Chlamydia trachomatis, C. pneumoniae, and IL-6 and IL-8 Gene alterations with heart diseases |
| title_short |
Association of Chlamydia trachomatis, C. pneumoniae, and IL-6 and IL-8 Gene alterations with heart diseases |
| title_full |
Association of Chlamydia trachomatis, C. pneumoniae, and IL-6 and IL-8 Gene alterations with heart diseases |
| title_fullStr |
Association of Chlamydia trachomatis, C. pneumoniae, and IL-6 and IL-8 Gene alterations with heart diseases |
| title_full_unstemmed |
Association of Chlamydia trachomatis, C. pneumoniae, and IL-6 and IL-8 Gene alterations with heart diseases |
| title_sort |
association of chlamydia trachomatis, c. pneumoniae, and il-6 and il-8 gene alterations with heart diseases |
| publisher |
Frontiers Media |
| publishDate |
2019 |
| url |
http://patua.iec.gov.br//handle/iec/3594 |
| _version_ |
1717584422229770240 |
| score |
11.755432 |