Plasmodium falciparum malarial parasites from Brazil lack artemisinin resistance-associated mutations in the kelch13 gene
INTRODUCTION: Mutations in the propeller domain of the Plasmodium falciparum kelch13 (k13) gene are associated with artemisinin resistance. METHODS: We developed a PCR protocol to sequence the pfk13 gene and determined its sequence in a batch of 50 samples collected from 2003 to 2016 in Brazil. RESU...
| Autor principal: | Chapadense, Francesca |
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| Outros Autores: | Machado, Ricardo Luiz Dantas, Ventura, Ana Maria Revor?do da Silva, ?reas, Andr?, Machado, Renato Beilner, Vianna, Giselle Maria Rachid, Zalis, Mariano Gustavo, Castro, Ana Maria de, Cravo, Pedro |
| Grau: | Artigo |
| Idioma: | eng |
| Publicado em: |
Sociedade Brasileira de Medicina Tropical
2019
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| Acesso em linha: |
http://patua.iec.gov.br//handle/iec/3621 |
| Resumo: |
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INTRODUCTION: Mutations in the propeller domain of the Plasmodium falciparum kelch13 (k13) gene are associated with artemisinin resistance. METHODS: We developed a PCR protocol to sequence the pfk13 gene and determined its sequence in a batch of 50 samples collected from 2003 to 2016 in Brazil. RESULTS: We identified 1 K189T substitution located outside the propeller domain of the PfK13 protein in 36% of samples. CONCLUSIONS: Although the sample size is relatively small, these results suggest that P. falciparum artemisinin-resistant mutants do not exist in Brazil, thereby supporting the continuation of current treatment programs based on artemisinin-based combination therapy. |