Rotavirus antigenemia as a common event among children hospitalised for severe, acute gastroenteritis in Bel?m, northern Brazil
Background: Rotavirus antigenemia and RNAemia (the presence of rotavirus RNA in serum) have been commonly identified among paediatric patients with acute gastroenteritis. In this study we examined the association between rotavirus antigenemia and clinical features, and sought to determine the geno...
| Autor principal: | Justino, Maria Cleonice Aguiar |
|---|---|
| Outros Autores: | Campos, Erika A, Mascarenhas, Joana D'Arc Pereira, Soares, Luana da Silva, Guerra, Sylvia de F?tima dos Santos, Furlaneto, Ismari Perini, Pav?o Jr, Manoel Jaime C, Maciel, Tassio S, Farias, Fredison P, Bezerra, Orv?cio Melo, Vinente, Caio Breno G, Barros, Rodrigo Jos? S, Linhares, Alexandre da Costa |
| Grau: | Artigo |
| Idioma: | eng |
| Publicado em: |
BioMed Central
2019
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| Acesso em linha: |
http://patua.iec.gov.br//handle/iec/3759 |
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ir-iec-3759 |
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ir-iec-37592019-09-10T18:31:01Z Rotavirus antigenemia as a common event among children hospitalised for severe, acute gastroenteritis in Bel?m, northern Brazil Justino, Maria Cleonice Aguiar Campos, Erika A Mascarenhas, Joana D'Arc Pereira Soares, Luana da Silva Guerra, Sylvia de F?tima dos Santos Furlaneto, Ismari Perini Pav?o Jr, Manoel Jaime C Maciel, Tassio S Farias, Fredison P Bezerra, Orv?cio Melo Vinente, Caio Breno G Barros, Rodrigo Jos? S Linhares, Alexandre da Costa Infec??es por Rotavirus / virologia Gastroenterite / virologia Rea??o em Cadeia da Polimerase / m?todos Monitoramento Epidemiol?gico Antigenemia Hospitaliza??o Background: Rotavirus antigenemia and RNAemia (the presence of rotavirus RNA in serum) have been commonly identified among paediatric patients with acute gastroenteritis. In this study we examined the association between rotavirus antigenemia and clinical features, and sought to determine the genotypes of rotaviruses detected in paired stool and serum samples. Methods: Paired stool and serum samples were obtained from children hospitalised for acute gastroenteritis in Bel?m, Brazil, between June 2012 and June 2015. The 20-point Vesikari scoring system was used to assess the disease severity upon a retrospective medical record review. Stool and serum samples were primarily screened for the presence of rotavirus antigen using a commercial ELISA assay. The rotavirus isolates from stool and serum samples were genotyped by using the classical reverse-transcriptase polymerase chain reaction (RT-PCR) and/or through nucleotide sequencing of VP4 and VP7 genes. Viral load was estimated using real-time RT-PCR. Results: In total rotavirus antigen was detected in 109 (24.2%) stool samples from 451 children, whereas antigenemia occurred in 38.5% (42/109) of these patients. We demonstrated that patients positive for rotavirus RNA in paired stool and serum samples were more likely to have a higher frequency of vomiting episodes in a 24-h period (p = 0.0035). Our findings also suggested that children not vaccinated against rotavirus are more likely to develop antigenemia, as compared to those given at least one vaccine dose (p = 0.0151). G12P [8] and G2P [4] genotypes were predominant throughout the study period, accounting for 52.3% (57/109) and 27.5% (30/109) of the typed isolates, respectively. Ten stool-serum pairs could be typed for VP4 and VP7 genes. Seven of these pairs showed concordant results with G2P [4] genotype being detected in stool and serum samples, whereas discrepancies between genotypes (G2P [4]/G2P[NT] and G12P [8]/G2P[NT]) were seen in three pairs. Conclusions: Rotavirus antigenemia and RNAemia occur in a significant number of children hospitalised for acute gastroenteritis in Bel?m, Brazil, and may contribute to a greater disease severity, particularly translated into a greater number of vomiting episodes. This study documented a high concordance of genotypes detected in a subgroup of paired stool and serum samples This study received financial support from the Evandro Chagas Institute (IEC), Health Surveillance Secretariat, which supported the study team to perform sample collection, analysis, interpretation of the data obtained and writing the manuscript. The National Council for Scientific and Technological Development (CNPq) provided financial support to purchase laboratory kits for use in the analysis. 2019-06-18T18:47:31Z 2019-06-18T18:47:31Z 2019 Artigo JUSTINO, Maria Cleonice Aguiar et al. Rotavirus antigenemia as a common event among children hospitalised for severe, acute gastroenteritis in Bel?m, northern Brazil. BMC Pediatrics, v.19, n. 1, p. 1-11, 2019. 1471-2431 http://patua.iec.gov.br//handle/iec/3759 10.1186/s12887-019-1535-2 eng Acesso Aberto application/pdf BioMed Central |
| institution |
Instituto Evandro Chagas (IEC) |
| collection |
PATUA |
| language |
eng |
| topic |
Infec??es por Rotavirus / virologia Gastroenterite / virologia Rea??o em Cadeia da Polimerase / m?todos Monitoramento Epidemiol?gico Antigenemia Hospitaliza??o |
| spellingShingle |
Infec??es por Rotavirus / virologia Gastroenterite / virologia Rea??o em Cadeia da Polimerase / m?todos Monitoramento Epidemiol?gico Antigenemia Hospitaliza??o Justino, Maria Cleonice Aguiar Rotavirus antigenemia as a common event among children hospitalised for severe, acute gastroenteritis in Bel?m, northern Brazil |
| topic_facet |
Infec??es por Rotavirus / virologia Gastroenterite / virologia Rea??o em Cadeia da Polimerase / m?todos Monitoramento Epidemiol?gico Antigenemia Hospitaliza??o |
| description |
Background: Rotavirus antigenemia and RNAemia (the presence of rotavirus RNA in serum) have been commonly
identified among paediatric patients with acute gastroenteritis. In this study we examined the association between
rotavirus antigenemia and clinical features, and sought to determine the genotypes of rotaviruses detected in
paired stool and serum samples.
Methods: Paired stool and serum samples were obtained from children hospitalised for acute gastroenteritis in Bel?m,
Brazil, between June 2012 and June 2015. The 20-point Vesikari scoring system was used to assess the disease severity
upon a retrospective medical record review. Stool and serum samples were primarily screened for the presence of
rotavirus antigen using a commercial ELISA assay. The rotavirus isolates from stool and serum samples were genotyped
by using the classical reverse-transcriptase polymerase chain reaction (RT-PCR) and/or through nucleotide sequencing
of VP4 and VP7 genes. Viral load was estimated using real-time RT-PCR.
Results: In total rotavirus antigen was detected in 109 (24.2%) stool samples from 451 children, whereas antigenemia
occurred in 38.5% (42/109) of these patients. We demonstrated that patients positive for rotavirus RNA in paired stool
and serum samples were more likely to have a higher frequency of vomiting episodes in a 24-h period (p = 0.0035).
Our findings also suggested that children not vaccinated against rotavirus are more likely to develop antigenemia, as
compared to those given at least one vaccine dose (p = 0.0151). G12P [8] and G2P [4] genotypes were predominant
throughout the study period, accounting for 52.3% (57/109) and 27.5% (30/109) of the typed isolates, respectively. Ten
stool-serum pairs could be typed for VP4 and VP7 genes. Seven of these pairs showed concordant results with G2P [4]
genotype being detected in stool and serum samples, whereas discrepancies between genotypes (G2P [4]/G2P[NT]
and G12P [8]/G2P[NT]) were seen in three pairs.
Conclusions: Rotavirus antigenemia and RNAemia occur in a significant number of children hospitalised for acute
gastroenteritis in Bel?m, Brazil, and may contribute to a greater disease severity, particularly translated into a greater
number of vomiting episodes. This study documented a high concordance of genotypes detected in a subgroup of
paired stool and serum samples |
| format |
Artigo |
| author |
Justino, Maria Cleonice Aguiar |
| author2 |
Campos, Erika A Mascarenhas, Joana D'Arc Pereira Soares, Luana da Silva Guerra, Sylvia de F?tima dos Santos Furlaneto, Ismari Perini Pav?o Jr, Manoel Jaime C Maciel, Tassio S Farias, Fredison P Bezerra, Orv?cio Melo Vinente, Caio Breno G Barros, Rodrigo Jos? S Linhares, Alexandre da Costa |
| author2Str |
Campos, Erika A Mascarenhas, Joana D'Arc Pereira Soares, Luana da Silva Guerra, Sylvia de F?tima dos Santos Furlaneto, Ismari Perini Pav?o Jr, Manoel Jaime C Maciel, Tassio S Farias, Fredison P Bezerra, Orv?cio Melo Vinente, Caio Breno G Barros, Rodrigo Jos? S Linhares, Alexandre da Costa |
| title |
Rotavirus antigenemia as a common event among children hospitalised for severe, acute gastroenteritis in Bel?m, northern Brazil |
| title_short |
Rotavirus antigenemia as a common event among children hospitalised for severe, acute gastroenteritis in Bel?m, northern Brazil |
| title_full |
Rotavirus antigenemia as a common event among children hospitalised for severe, acute gastroenteritis in Bel?m, northern Brazil |
| title_fullStr |
Rotavirus antigenemia as a common event among children hospitalised for severe, acute gastroenteritis in Bel?m, northern Brazil |
| title_full_unstemmed |
Rotavirus antigenemia as a common event among children hospitalised for severe, acute gastroenteritis in Bel?m, northern Brazil |
| title_sort |
rotavirus antigenemia as a common event among children hospitalised for severe, acute gastroenteritis in bel?m, northern brazil |
| publisher |
BioMed Central |
| publishDate |
2019 |
| url |
http://patua.iec.gov.br//handle/iec/3759 |
| _version_ |
1717584477977313280 |
| score |
11.755432 |