Physalis angulata induces death of promastigotes and amastigotes of Leishmania (Leishmania) amazonensis via the generation of reactive oxygen species

Silva, Bruno Jos? Martins da; Silva, Raquel Raick P. da; Farias, Ana Paula Drummond Rodrigues de; Farias, Luis Henrique Seabra; Nascimento, Jos? Luiz Martins do; Silva, Edilene Oliveira

Artigo

Physalis angulata induces death of promastigotes and amastigotes of Leishmania (Leishmania) amazonensis via the generation of reactive oxygen species

Leishmaniasis are a neglected group of emerging diseases that have been found in 98 countries and are caused by protozoa of the genus Leishmania. The therapy for leishmaniasis causes several side effects and leads to drug-resistant strains. Natural products from plants have exhibited activities agai...

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Autor principal:	Silva, Bruno Jos? Martins da
Outros Autores:	Silva, Raquel Raick P. da, Farias, Ana Paula Drummond Rodrigues de, Farias, Luis Henrique Seabra, Nascimento, Jos? Luiz Martins do, Silva, Edilene Oliveira
Grau:	Artigo
Idioma:	eng
Publicado em:	Elsevier 2019
Assuntos:	Physalis / qu?mica Leishmaniose / parasitologia Leishmaniose / tratamento farmacol?gico Plantas Medicinais Ativa??o de Macr?fagos / efeitos dos f?rmacos Esp?cies Reativas de Oxig?nio
Acesso em linha:	http://patua.iec.gov.br//handle/iec/3816

id	ir-iec-3816
recordtype	dspace
spelling	ir-iec-38162019-07-08T14:26:56Z Physalis angulata induces death of promastigotes and amastigotes of Leishmania (Leishmania) amazonensis via the generation of reactive oxygen species Silva, Bruno Jos? Martins da Silva, Raquel Raick P. da Farias, Ana Paula Drummond Rodrigues de Farias, Luis Henrique Seabra Nascimento, Jos? Luiz Martins do Silva, Edilene Oliveira Physalis / qu?mica Leishmaniose / parasitologia Leishmaniose / tratamento farmacol?gico Plantas Medicinais Ativa??o de Macr?fagos / efeitos dos f?rmacos Esp?cies Reativas de Oxig?nio Leishmaniasis are a neglected group of emerging diseases that have been found in 98 countries and are caused by protozoa of the genus Leishmania. The therapy for leishmaniasis causes several side effects and leads to drug-resistant strains. Natural products from plants have exhibited activities against Leishmania in various experimental models. Physalis angulata is a widely used plant in popular medicine, and in the literature it has well-documented leishmanicidal activity. However, its mechanism of action is still unknown. Thus, this study aims to evaluate the mechanism driving the leishmanicidal activity of an aqueous extract of P. angulata root (AEPa). AEPa was effective against both promastigotes and intracellular amastigote forms of Leishmania amazonensis. This effect was mediated by an increase of reactive oxygen species (ROS), but not of nitric oxide (NO). The increased production of ROS induces cell death by phenotypes seems by apoptosis cell death in Leishmania, but not autophagy or necrosis. In addition, morphological analysis of macrophages showed that AEPa induced a high number of cytoplasmic projections, increased the volume of cytoplasm and number of vacuoles, caused cytoskeleton alterations and resulted in high spreading ability. AEPa also promoted superoxide anion (O2(-)) production in both uninfected macrophages and those infected with Leishmania. Therefore, these results revealed that AEPa causes cell death by phenotypes seems by apoptosis cell death in L. amazonensis and modulates macrophage activation through morphofunctional alterations and O2(-) generation to induce Leishmania death. 2019-07-08T14:04:06Z 2019-07-08T14:04:06Z 2016 Artigo SILVA, Bruno Jos? Martins da et al. Physalis angulata induces death of promastigotes and amastigotes of Leishmania (Leishmania) amazonensis via the generation of reactive oxygen species. Micron, v. 82, n. x, p. 25-32, Mar. 2016. 0968-4328 http://patua.iec.gov.br//handle/iec/3816 10.1016/j.micron.2015.12.001 eng Acesso Embargado application/pdf Elsevier
institution	Instituto Evandro Chagas (IEC)
collection	PATUA
language	eng
topic	Physalis / qu?mica Leishmaniose / parasitologia Leishmaniose / tratamento farmacol?gico Plantas Medicinais Ativa??o de Macr?fagos / efeitos dos f?rmacos Esp?cies Reativas de Oxig?nio
spellingShingle	Physalis / qu?mica Leishmaniose / parasitologia Leishmaniose / tratamento farmacol?gico Plantas Medicinais Ativa??o de Macr?fagos / efeitos dos f?rmacos Esp?cies Reativas de Oxig?nio Silva, Bruno Jos? Martins da Physalis angulata induces death of promastigotes and amastigotes of Leishmania (Leishmania) amazonensis via the generation of reactive oxygen species
topic_facet	Physalis / qu?mica Leishmaniose / parasitologia Leishmaniose / tratamento farmacol?gico Plantas Medicinais Ativa??o de Macr?fagos / efeitos dos f?rmacos Esp?cies Reativas de Oxig?nio
description	Leishmaniasis are a neglected group of emerging diseases that have been found in 98 countries and are caused by protozoa of the genus Leishmania. The therapy for leishmaniasis causes several side effects and leads to drug-resistant strains. Natural products from plants have exhibited activities against Leishmania in various experimental models. Physalis angulata is a widely used plant in popular medicine, and in the literature it has well-documented leishmanicidal activity. However, its mechanism of action is still unknown. Thus, this study aims to evaluate the mechanism driving the leishmanicidal activity of an aqueous extract of P. angulata root (AEPa). AEPa was effective against both promastigotes and intracellular amastigote forms of Leishmania amazonensis. This effect was mediated by an increase of reactive oxygen species (ROS), but not of nitric oxide (NO). The increased production of ROS induces cell death by phenotypes seems by apoptosis cell death in Leishmania, but not autophagy or necrosis. In addition, morphological analysis of macrophages showed that AEPa induced a high number of cytoplasmic projections, increased the volume of cytoplasm and number of vacuoles, caused cytoskeleton alterations and resulted in high spreading ability. AEPa also promoted superoxide anion (O2(-)) production in both uninfected macrophages and those infected with Leishmania. Therefore, these results revealed that AEPa causes cell death by phenotypes seems by apoptosis cell death in L. amazonensis and modulates macrophage activation through morphofunctional alterations and O2(-) generation to induce Leishmania death.
format	Artigo
author	Silva, Bruno Jos? Martins da
author2	Silva, Raquel Raick P. da Farias, Ana Paula Drummond Rodrigues de Farias, Luis Henrique Seabra Nascimento, Jos? Luiz Martins do Silva, Edilene Oliveira
author2Str	Silva, Raquel Raick P. da Farias, Ana Paula Drummond Rodrigues de Farias, Luis Henrique Seabra Nascimento, Jos? Luiz Martins do Silva, Edilene Oliveira
title	Physalis angulata induces death of promastigotes and amastigotes of Leishmania (Leishmania) amazonensis via the generation of reactive oxygen species
title_short	Physalis angulata induces death of promastigotes and amastigotes of Leishmania (Leishmania) amazonensis via the generation of reactive oxygen species
title_full	Physalis angulata induces death of promastigotes and amastigotes of Leishmania (Leishmania) amazonensis via the generation of reactive oxygen species
title_fullStr	Physalis angulata induces death of promastigotes and amastigotes of Leishmania (Leishmania) amazonensis via the generation of reactive oxygen species
title_full_unstemmed	Physalis angulata induces death of promastigotes and amastigotes of Leishmania (Leishmania) amazonensis via the generation of reactive oxygen species
title_sort	physalis angulata induces death of promastigotes and amastigotes of leishmania (leishmania) amazonensis via the generation of reactive oxygen species
publisher	Elsevier
publishDate	2019
url	http://patua.iec.gov.br//handle/iec/3816
_version_	1717584478541447168
score	11.653393

Physalis angulata induces death of promastigotes and amastigotes of Leishmania (Leishmania) amazonensis via the generation of reactive oxygen species

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