Currently only four genome sequences for Limnothrix spp. are publicly available, and
information on the genetic properties of cyanobacteria belonging to this genus is limited.
In this study, we report the draft genome of Limnothrix sp. CACIAM 69d, isolated
from the reservoir of a hydroelectric dam located in the Amazon ecosystem, from
where cyanobacterial genomic data are still scarce. Comparative genomic analysis
of Limnothrix revealed the presence of key enzymes in the cyanobacterial central
carbon metabolism and how it is well equipped for environmental sulfur and nitrogen
acquisition. Additionally, this work covered the analysis of Limnothrix CRISPR-Cas
systems, pathways related to biosynthesis of secondary metabolites and assembly
of extracellular polymeric substances and their exportation. A trans-AT PKS gene
cluster was identified in two strains, possibly related to the novel toxin Limnothrixin
biosynthesis. Overall, the draft genome of Limnothrix sp. CACIAM 69d adds new data
to the small Limnothrix genome library and contributes to a growing representativeness
of cyanobacterial genomes from the Amazon region. The comparative genomic analysis
of Limnothrix made it possible to highlight unique genes for each strain and understand
the overall features of their metabolism.
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