Molecular characterization of Babesia vogeli in dogs from Bel?m, northern Brazil
Babesiosis is an infectious hemolytic disease that occurs worldwide, and is caused by a protozoan of the Babesia genus (Apicomplexa). Little is known about this genus; therefore, this study conducted a molecular characterization of Babesia spp in naturally infected dogs in northern Brazil. Blood sam...
| Autor principal: | Moraes, Pablo H. G |
|---|---|
| Outros Autores: | Rufino, C. P, Bara?na, A. R. F, Reis, T, Agnol, L. T. D, Meneses, A. M. C, Aguiar, D?lia Cristina Figueira, Nunes, Marcio Roberto Teixeira, Gon?alves, Evonnildo Costa |
| Grau: | Artigo |
| Idioma: | eng |
| Publicado em: |
Funpec-Rp
2019
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| Assuntos: | |
| Acesso em linha: |
http://patua.iec.gov.br//handle/iec/3864 |
| id |
ir-iec-3864 |
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| recordtype |
dspace |
| spelling |
ir-iec-38642019-08-28T16:55:13Z Molecular characterization of Babesia vogeli in dogs from Bel?m, northern Brazil Moraes, Pablo H. G Rufino, C. P Bara?na, A. R. F Reis, T Agnol, L. T. D Meneses, A. M. C Aguiar, D?lia Cristina Figueira Nunes, Marcio Roberto Teixeira Gon?alves, Evonnildo Costa Babesiose / parasitologia Babesiose / epidemiologia Doen?as do C?o / parasitologia Doen?as do C?o / epidemiologia Doen?as Transmitidas por Carrapatos Babesia / isolamento & purifica??o Rhipicephalus sanguineus An?lise de Sequ?ncia de DNA Rea??o em Cadeia da Polimerase / m?todos Babesiosis is an infectious hemolytic disease that occurs worldwide, and is caused by a protozoan of the Babesia genus (Apicomplexa). Little is known about this genus; therefore, this study conducted a molecular characterization of Babesia spp in naturally infected dogs in northern Brazil. Blood samples were collected from 172 dogs from metropolitan Bel?m, and screened for Babesia spp using semi-nested polymerase chain reactions. Subsequently, 18S rDNA gene fragments were amplified and sequenced. Alignments of the 27 nucleotide sequences returned fragments measuring 1023 bp in size, which did not include any polymorphic sites (100 per cent identity). This genotype was very similar to the 18S rDNA gene in B. vogeli. This study provides an important molecular characterization of the parasite responsible for canine babesiosis in naturally infected dogs in metropolitan Bel?m. In addition, the 18S rDNA gene in B. vogeli may represent the most common genotype occurring in South America. 2019-08-28T16:47:52Z 2019-08-28T16:47:52Z 2015 Artigo MORAES, Pablo H. G. et al. Molecular characterization of Babesia vogeli in dogs from Bel?m, northern Brazil. Genetics and Molecular Research. (Online), v. 14, n. 4, p. 16364-16371, Dec. 2015. 1676-5680 http://patua.iec.gov.br//handle/iec/3864 10.4238/2015.December.9.4 eng Acesso Aberto application/pdf Funpec-Rp |
| institution |
Instituto Evandro Chagas (IEC) |
| collection |
PATUA |
| language |
eng |
| topic |
Babesiose / parasitologia Babesiose / epidemiologia Doen?as do C?o / parasitologia Doen?as do C?o / epidemiologia Doen?as Transmitidas por Carrapatos Babesia / isolamento & purifica??o Rhipicephalus sanguineus An?lise de Sequ?ncia de DNA Rea??o em Cadeia da Polimerase / m?todos |
| spellingShingle |
Babesiose / parasitologia Babesiose / epidemiologia Doen?as do C?o / parasitologia Doen?as do C?o / epidemiologia Doen?as Transmitidas por Carrapatos Babesia / isolamento & purifica??o Rhipicephalus sanguineus An?lise de Sequ?ncia de DNA Rea??o em Cadeia da Polimerase / m?todos Moraes, Pablo H. G Molecular characterization of Babesia vogeli in dogs from Bel?m, northern Brazil |
| topic_facet |
Babesiose / parasitologia Babesiose / epidemiologia Doen?as do C?o / parasitologia Doen?as do C?o / epidemiologia Doen?as Transmitidas por Carrapatos Babesia / isolamento & purifica??o Rhipicephalus sanguineus An?lise de Sequ?ncia de DNA Rea??o em Cadeia da Polimerase / m?todos |
| description |
Babesiosis is an infectious hemolytic disease that occurs worldwide, and is caused by a protozoan of the Babesia genus (Apicomplexa). Little is known about this genus; therefore, this study conducted a molecular characterization of Babesia spp in naturally infected dogs in northern Brazil. Blood samples were collected from 172 dogs from metropolitan Bel?m, and screened for Babesia spp using semi-nested polymerase chain reactions. Subsequently, 18S rDNA gene fragments were amplified and sequenced. Alignments of the 27 nucleotide sequences returned fragments measuring 1023 bp in size, which did not include any polymorphic sites (100 per cent identity). This genotype was very similar to the 18S rDNA gene in B. vogeli. This study provides an important molecular characterization of the parasite responsible for canine babesiosis in naturally infected dogs in metropolitan Bel?m. In addition, the 18S rDNA gene in B. vogeli may represent the most common genotype occurring in South America. |
| format |
Artigo |
| author |
Moraes, Pablo H. G |
| author2 |
Rufino, C. P Bara?na, A. R. F Reis, T Agnol, L. T. D Meneses, A. M. C Aguiar, D?lia Cristina Figueira Nunes, Marcio Roberto Teixeira Gon?alves, Evonnildo Costa |
| author2Str |
Rufino, C. P Bara?na, A. R. F Reis, T Agnol, L. T. D Meneses, A. M. C Aguiar, D?lia Cristina Figueira Nunes, Marcio Roberto Teixeira Gon?alves, Evonnildo Costa |
| title |
Molecular characterization of Babesia vogeli in dogs from Bel?m, northern Brazil |
| title_short |
Molecular characterization of Babesia vogeli in dogs from Bel?m, northern Brazil |
| title_full |
Molecular characterization of Babesia vogeli in dogs from Bel?m, northern Brazil |
| title_fullStr |
Molecular characterization of Babesia vogeli in dogs from Bel?m, northern Brazil |
| title_full_unstemmed |
Molecular characterization of Babesia vogeli in dogs from Bel?m, northern Brazil |
| title_sort |
molecular characterization of babesia vogeli in dogs from bel?m, northern brazil |
| publisher |
Funpec-Rp |
| publishDate |
2019 |
| url |
http://patua.iec.gov.br//handle/iec/3864 |
| _version_ |
1717584400377446400 |
| score |
11.755432 |